| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We constructed wild type CCR5(CCR5-WT) and mutant CCR5(CCR5-MT), which loses susceptibility against HIV, coexpressing cells and tested HIV infectivity using these cells.10% and 50% levels of CCR5-MT expression reduced the susceptibility against HIV by 50% and almost 100% compared with only CCR5-WT espressing cells, respectively. This result strongly suggested that multiple CCR5 are required for the HIV entry step. Next we developed a mathematical model based on the correlation between the ratio of CCR5-MT expression and HIV susceptibility, and estimated the numbers of CCR5 required for the HIV infection. As a result we quantitatively estimated 6-8 CCR5s are required for gp120 trimer-CCR5 interaction. These data revealed a part of the entry step of HIV infection.
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