| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Antibiotic resistant cells emerge spontaneously at a certain frequency in the bacterial population that is termed as hetero-resistance. One of the most problematic antibiotic resistant bacteria in the clinical setting may be Staphylococcus aureus MRSA that exhibits resistance to structurally diverse multiple antibiotics including vancomycin. Among them, vancomycin intermediate resistant cells (VISA) appear in the population of vancomycin heteroresistant cells (hVISA). Therefore, detection of VISA cells in the hVISA population is difficult due to the fact that VISA appears at the frequency of 0.012% of hVISA. To detect VISA by the MIC method, over 10 times of cells are required compared with that of the conventional MIC determination. To overcome this difficulty, studies are on progress to detect VISA specific proteins by using MALDI-TOF-Mass spectroscopy.
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