Elucidation of construction abnormality of brain cytokine network caused by juvenile stress

• Project/Area Number: 26461521
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Teikyo University
• Principal Investigator: HIRASAWA Takae 帝京大学, 理工学部, 講師 (10402083)
• Co-Investigator(Kenkyū-buntansha): 中川 竜介 慶應義塾大学, 医学部(信濃町), 特任准教授 (10360603) ; 葛西 宏威 山梨大学, 総合研究部, 助教 (20324189)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 母子分離ストレス / ミクログリア / 母子分離 / 脳機能 / 免疫制御

Outline of Final Research Achievements

The brain, spleen, liver, and the bone marrow in the mouse gave maternal separation stress for seven days were harvested for this study. As the results showed, the microglia in separation group was the F4/80 negative cell while the microglia cell in control group was F4/80-positive cells. The expression of the microglial specific protein significantly decreased in the brain by the western blotting in separation group. However there were no significant effect　in spleen.
Moreover, the cell properties of the microglial cells isolated a pure microglial cells by FACS method. As for the isolated microglial cell, the property parts from FACS analysis for three group populations, and it is suggested that the origin of microglia may change by pre or postnatal stress and it may affect to the adult stage brain immunity.

Research Products

• [Journal Article] Protein-restricted diet during pregnancy after insemination alters behavioral phenotypes of the progeny (2017)
  • Author(s): Furuse T, Miyake K, Kohda T, Kaneda H, Hirasawa T, Yamada I, Kushida T, Kashimura M, Kobayashi K, Ishino F, Kubota T, Wakana S.
  • Journal Title: Genes & Nutrition Volume: 12 Issue: 1 Pages: 1-1
  • DOI: 10.1186/s12263-016-0550-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Long-term imipramine treatment increases N-methyl-d-aspartate receptor activity and expression via epigenetic mechanisms (2015)
  • Author(s): Nghia NA, Hirasawa T, Kasai H, Obata C, Moriishi K, Mochizuki K, Koizumi S, Kubota T.
  • Journal Title: Eur J Pharmacol. Volume: 752C Pages: 69-77
  • DOI: 10.1016/j.ejphar.2015.02.010
  • Peer Reviewed
• [Journal Article] Maternal restraint stress during pregnancy in mice induces 11β-HSD1-associated metabolic changes in the livers of the offspring. (2015)
  • Author(s): Maeyama H, Hirasawa T, Tahara Y, Obata C, Kasai H, Moriishi K, Mochizuki K, Kubota T.
  • Journal Title: J Dev Orig Health Dis. Volume: 6(2) Issue: 2 Pages: 105-114
  • DOI: 10.1017/s2040174415000100
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Synaptic dysfunction in the Glp Knockout mouse model of Kllefstra syndrome. (2016)
  • Author(s): Takae Hirasawa, Ayumi Yamada, Madoka Kato, Yoichi Shinkai, Takeo Kubota.
  • Organizer: 第39回日本神経科学学会
  • Place of Presentation: 横浜（パシフィコ横浜）
  • Int'l Joint Research
• [Presentation] Proteasome Activator PA28 gamma regulates neuronal function of cerebellar Purkinje cells. (2016)
  • Author(s): 中村和純, 天笠太一, 鶴岡直人, 葛西宏威, 森石恆司, 平澤孝枝
  • Organizer: 第39回日本分子生物学会
  • Place of Presentation: 横浜（パシフィコ横浜）
• [Presentation] 幼若期ストレスにおけるストレス耐性機能の獲得とミクログリア (2015)
  • Author(s): 平澤孝枝
  • Organizer: BMB2015第38回日本分子生物学会/第88回日本生化学会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-02
• [Presentation] The effects of maternal malnutrition in utero on gene expression, genomic methylation and behavioral phenotypes in mouse. (2015)
  • Author(s): Tamio Furuse, Takashi Kohda, Kunio Miyake, Takae Hirasawa, Tomoko Kushida
  • Organizer: 第38回日本神経科学学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2015-07-28
• [Presentation] Long-term imipramine treatment up-regulates NMDA receptors by epigenetic mechanisms in mouse cortical cultured neurons. (2014)
  • Author(s): Nghia Nguyen, Takae Hirasawa, Hirotake Kasai, Chie Obata, Kohji Moriishi, Kazuki Mochizuki, Takeo Kubota.
  • Organizer: 第37 回日本神経科学学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-09-12
• [Presentation] 胎児期ストレスとエピジェネィクス変化 (2014)
  • Author(s): 平澤孝枝
  • Organizer: 第4回日本DOHaD研究会学術集会
  • Place of Presentation: 国立成育医療センター（東京世田谷区）
  • Year and Date: 2014-07-26
  • Invited
• [Remarks] 帝京大学バイオサイエンス学科
  • URL: https://www.e-campus.gr.jp/staffinfo/public/staff/detail/2408/32
• [Remarks] 帝京大学理工学部バイオサイエンス学科神経発達生理学研究室
  • URL: https://www.teikyo-u.ac.jp/faculties/undergraduate/science_tech/labo.html#head1_4
• [Remarks]
  • URL: https://www.teikyo-u.ac.jp/faculties/undergraduate/science_tech/bio_science/
• [Remarks]
  • URL: http://www.jst.go.jp/kisoken/crest/project/34/34_12.html