| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In this study, we focus on the major polyunsaturated fatty acids(PUFAs) in human breast milk: linoleic acid(LA), alpha-linolenic acid (ALA), arachidonic acid (AA), and docosahexaenoic acid (DHA) in views of the effect of breast feeding on the lipid metabolism in baby. All experiments were performed using 3T3-L1 adipocyte cell line. Our results are as follows: 1. All PUFA (LA ALA AA and DHA) suppressed the gene expression of SCD1 gene. SCD1 is the lipogenic gene and the suppression of SCD1 could inhibit the progression to metabolic disorders including insulin resistance, diabetes and metabolic syndrome. 2. the mixture of multiple PUFA (e.g. LA and AA, or ALA and AA) suppressed SCD1 gene expression synergistically3 Some part of the suppressive effect by PUFAs is depend in PPARg pathway. We plan to proceed comprehensive gene expression analysis and lipidomic studies analysis to figure out the mechanism of PUFA induced SCD1 suppression.
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