| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The aim of this study was to identify novel lipid molecules whose amounts vary to reflect the status of peroxisomal disease and apply them as a biomarker. We established analysis methods for sphingomyelin and acyl-CoA, as well as a method for comprehensive analysis of glycosphingolipids using mass spectrometry. We analyzed phospholipids from the brain of ABCD1-knockout mice and found an increase in several numbers of species containing very long chain fatty acids. We then determined the molecules’ structures. In addition, we have established an ABCD1-knockout cell line. We have started the identification of a responsive enzyme for the synthesis of phospholipid species containing very long chain fatty acids.
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