Elucidation of peroxisomal disease mechanisms by analyzing novel lipid molecules in the patient's serum and applying them as a biomarker
| Project/Area Number |
26461532
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Research Collaborator |
SHIMOZAWA Nobuyuki
IMANAKA Tsuneo
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | リピドミクス / メタボローム / 極長鎖脂肪酸 / 副腎白質ジストロフィー / ペルオキシソーム / 質量分析 / 先天代謝異常 / リン脂質 / ペルオキシソーム病 / ABCD1/ALDP / 脂質メタボローム / 質量分析法 |
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| Outline of Final Research Achievements |
The aim of this study was to identify novel lipid molecules whose amounts vary to reflect the status of peroxisomal disease and apply them as a biomarker. We established analysis methods for sphingomyelin and acyl-CoA, as well as a method for comprehensive analysis of glycosphingolipids using mass spectrometry. We analyzed phospholipids from the brain of ABCD1-knockout mice and found an increase in several numbers of species containing very long chain fatty acids. We then determined the molecules’ structures. In addition, we have established an ABCD1-knockout cell line. We have started the identification of a responsive enzyme for the synthesis of phospholipid species containing very long chain fatty acids.
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Report
(4 results)
Research Products
(16 results)
