| Project/Area Number |
26461553
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
鈴木 潤一 日本大学, 医学部, 助教 (00573825)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2016: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 小児 / 1型糖尿病 / micro RNA / 発症機序 / 発症予防 / micro RNA多型 / 膵島関連自己抗体 / インスリン分泌能 / 遺伝子多型 |
|---|
| Outline of Final Research Achievements |
One hundred eighty seven children with type 1 diabetes, who showed positive for β-cell associated antibodies and exhausted endogenous insulin secretion, were studied. Using their stored DNA, we performed amplicon-based assay for targeted resequencing of the DNA, targeting the 69 candidate micro RNAs, with next generation sequencer. We conducted this procedure twice, however,the results were not provided, because of poor clustering or excessive clustering from DNA samples. It was impossible to perform the same procedure using the same DNA samples. We therefore evaluated association between clinical features and vivo expression of micro RNA in newly diagnosed patients with type 1 diabetes.However, we did not find significant association between dose of expression of miRNA and all the clinical features, including onset-age, onset- type of diabetes, presence of β-cell associated antibodies and residual β-cell function.
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