The structural analysis of the onset mechanism of cryopyrin-associated periodic syndrome
Project/Area Number |
26461576
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pediatrics
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Research Institution | Gifu University |
Principal Investigator |
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Research Collaborator |
TOCHIO HIDEHITO 京都大学
KIMURA TAKESHI 岐阜大学
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 自己炎症性疾患 / 自然免疫 |
Outline of Final Research Achievements |
The purpose of this study was the recombinant protein expression, purification and molecular analyses of human NLRP3, in order to analysis the onset mechanism of cryopyrin-associated periodic syndrome. We tried to use the insect cell protein expression systems (silkworm or SF9 cells) with the various protein expression constructs. The protein expression of NLRP3 variants was confirmed, but they were insoluble. Therefore, we could not obtain the suitable protein samples for analyzing the function and structure. In conclusion, our study revealed the quite difficulty of the large-scale protein purification of human NLRP3.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Alanine-scanning mutagenesis of human signal transducer and activator of transcription 1 to estimate loss- or gain-of-function variants2016
Author(s)
Kagawa R, Fujiki R, Tsumura M, Sakata S, Nishimura S,Itan Y, Kong XF, Kato Z, Ohnishi H, Hirata O, Saito S, Ikeda M,Baghdadi JE, Bousfiha A, Fujiwara K, Oleastro M, Yancoski J,Perez L, Danielian S, Ailal F, Takada H, Hara T, Anne Puel A, Boisson-Dupuis S, Bustamante J, Casanova JL, Ohara O, Okada S, Masao Kobayashi M.
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Journal Title
J Allergy Clin Immunol.
Volume: -
Issue: 1
Pages: 232-241
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Mondini dysplasia with recurrent bacterial meningitis caused by three different pathogens.2015
Author(s)
Shikano H, Ohnishi H, Fukutomi H, Ito K, Morimoto M, Teramoto T, Aoki M, Nishihori T, Akeda Y, Oishi K, Fukao T.
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Journal Title
Pediatr Int.
Volume: 57
Issue: 6
Pages: 1192-1195
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] The structural basis for receptor recognition of human interleukin-182014
Author(s)
Naotaka Tsutsumi, Takeshi Kimura, Kyohei Arita, Mariko Ariyoshi, Hidenori Ohnishi, Takahiro Yamamoto, Xiaobing Zuo, Katsumi Maenaka, Enoch Y. Park, Naomi Kondo, Masahiro Shirakawa, Hidehito Tochio and Zenichiro Kato
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Journal Title
Nat. Commun.
Volume: 5 5340
Issue: 1
Pages: 1-13
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A complement factor B mutation in a large kindred with atypical hemolytic uremic syndrome.2014
Author(s)
Funato M, Uemura O, Ushijima K, Ohnishi H, Orii K, Kato Z, Yamakawa S, Nagai T, Ohara O, Kaneko H, Kondo N.
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Journal Title
J Clin Immunol.
Volume: 34
Issue: 6
Pages: 691-695
DOI
Related Report
Peer Reviewed
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