| Project/Area Number |
26461577
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Gifu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
恵良 聖一 岐阜大学, 大学院医学系研究科, 非常勤講師 (30152002)
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| Co-Investigator(Renkei-kenkyūsha) |
TOMIDA Mihoko 松本歯科大学, 歯学部, 教授 (00366329)
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| Research Collaborator |
ARIKAWA Hajime
TAKAHASHI Teppei
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 高親和性IgE受容体β鎖 / 生物物理 / シグナル伝達 / アレルギー・ぜんそく / 蛋白質 |
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| Outline of Final Research Achievements |
The high affinity IgE Fc receptor (FcεRI) β chain is an important molecule for signal transduction of mast cells. We confirmed the secondary, tertiary structure and thermal stability of β chain mutant (D234A) protein in which the 234th amino acid of C-terminal β chain was substituted with alanine, which is suggested to be involved in mast cell signal transduction. In addition, it was revealed that the β chain polymorphism (E228G) protein affects thermal stability, although there is no difference in the secondary and tertiary structure as compared with the wild type. The structure and thermodynamic properties of the β chain clarified in this study are expected to be useful for drug discovery targeting the β chain.
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