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Identification of molecules associated with high affinity IgE Fc receptor beta chain D234 and its application to therapy and drug discovery

Research Project

Project/Area Number 26461577
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pediatrics
Research InstitutionGifu University

Principal Investigator

TERADA TOMOYOSHI  岐阜大学, 医学部, 准教授 (30345780)

Co-Investigator(Kenkyū-buntansha) 恵良 聖一  岐阜大学, 大学院医学系研究科, 非常勤講師 (30152002)
Co-Investigator(Renkei-kenkyūsha) TOMIDA Mihoko  松本歯科大学, 歯学部, 教授 (00366329)
Research Collaborator ARIKAWA Hajime  
TAKAHASHI Teppei  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords高親和性IgE受容体β鎖 / 生物物理 / シグナル伝達 / アレルギー・ぜんそく / 蛋白質
Outline of Final Research Achievements

The high affinity IgE Fc receptor (FcεRI) β chain is an important molecule for signal transduction of mast cells. We confirmed the secondary, tertiary structure and thermal stability of β chain mutant (D234A) protein in which the 234th amino acid of C-terminal β chain was substituted with alanine, which is suggested to be involved in mast cell signal transduction. In addition, it was revealed that the β chain polymorphism (E228G) protein affects thermal stability, although there is no difference in the secondary and tertiary structure as compared with the wild type. The structure and thermodynamic properties of the β chain clarified in this study are expected to be useful for drug discovery targeting the β chain.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (25 results)

All 2016 2015 2014

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (16 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Improvement of the fraction of human mercaptalbumin on hemodialysis treatment using hydrogen-dissolved hemodialysis fluid: a prospective observational study2016

    • Author(s)
      Maeda K, Yoshizaki S, Iida T, Terada T, Era S, Sakashita K, Arikawa H
    • Journal Title

      Renal Replacement Therapy

      Volume: 2 Issue: 1 Pages: 42-42

    • DOI

      10.1186/s41100-016-0054-y

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of the conformation and thermal stability of the high-affinity IgE Fc receptor β chain polymorphic proteins2016

    • Author(s)
      Terada T, Takahashi T, Arikawa H, Era S
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: in press Issue: 7 Pages: 1356-1361

    • DOI

      10.1080/09168451.2016.1153958

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Quantitation of Oxidative Modifications of Commercial Human Albumin for Clinical Use2016

    • Author(s)
      Takahashi T, Terada T, Arikawa H, Kizaki K, Terawaki H, Imai H, Itoh Y, Era S
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 39 Issue: 3 Pages: 401-408

    • DOI

      10.1248/bpb.b15-00843

    • NAID

      130005132297

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of reduced coenzyme Q10 (ubiquinol) supplementation on blood pressure and muscle damage during kendo training camp: a double-blind, randomized controlled study2015

    • Author(s)
      Kizaki K, Terada T, Arikawa H, Tajima T, Imai H, Takahashi T, Era S
    • Journal Title

      J Sports Med Phys Fitness

      Volume: 55(7-8) Pages: 797-804

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Continuous Vocalization during Kendo Exercises Suppresses Expiration Of CO22015

    • Author(s)
      Arikawa H, Terada T, Takahashi T, Kizaki K, Imai H, Era S
    • Journal Title

      Int J Sports Med

      Volume: 36(7) Issue: 07 Pages: 519-525

    • DOI

      10.1055/s-0034-1398679

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of reduced coenzyme Q10 (ubiquinol) supplementation on blood pressure and muscle damage during kendo training camp: a double-blind, randomized controlled study2015

    • Author(s)
      Kizaki K, Terada T, Arikawa H, Tajima T, Imai H, Takahashi T, Era S
    • Journal Title

      J Sports Med Phys Fitness

      Volume: in press

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparative studies on the heterogeneity of plasma-derived and recombinant human albumins in laboratory use2014

    • Author(s)
      Minami T, Terada T, Takahashi T, Arikawa H, Matsuyama Y, Kizaki K, Era S
    • Journal Title

      Int J Biol Macromol

      Volume: 69 Pages: 79-87

    • DOI

      10.1016/j.ijbiomac.2014.05.010

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of a hydrogen (H2 )-enriched solution on the albumin redox of hemodialysis patients.2014

    • Author(s)
      Terawaki H, Zhu WJ, Matsuyama Y, Terada T, Takahashi Y, Sakurai K, Kabayama S, Miyazaki M, Itami N, Nakazawa R, Ito S, Era S, Nakayama M
    • Journal Title

      Hemodial Int

      Volume: In Press Issue: 2 Pages: 459-466

    • DOI

      10.1111/hdi.12112

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 血漿アルブミンの還元化率を改善する還元薬剤の透析液への添加とその有効濃度2014

    • Author(s)
      鈴木祥史、須田健二、松山幸枝、寺田知新、恵良聖一、副島昭典
    • Journal Title

      腎とフリーラジカル

      Volume: 12 Pages: 66-70

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 長期の水素溶存透析液による血液透析はアルブミンレドックスを改善する2016

    • Author(s)
      坂下惠一郎, 前田憲志, 飯田俊郎, 吉崎重仁, 寺田知新, 惠良聖一
    • Organizer
      第7回腎不全研究会
    • Place of Presentation
      東京
    • Year and Date
      2016-12-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] 電解水透析によるAlbumin redoxの改善~より長期の検討2016

    • Author(s)
      吉崎重仁, 近藤弥生, 加藤博愛, 平林雪江, 白川智栄, 中嶋俊介, 玉井 泉, 安藤律子, 稲田清美, 飯田俊郎, 前田憲志, 坂下惠一郎, 寺田知新, 惠良聖一
    • Organizer
      第93回東海透析研究会
    • Place of Presentation
      名古屋
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Improvement of the albumin redox state on hemodialysis treatment using hydrogen-dissolved hemodialysis fluid2016

    • Author(s)
      Sakashita K, Maeda K, Yosizaki S, Toshiro Iida T, Terada T, Seiichi Era S, Arikawa H
    • Organizer
      ISBP2016 34th Congress of International Society of Blood Purification
    • Place of Presentation
      Hiroshima
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 水素溶存透析液を用いた血液透析治療におけるアルブミン酸化還元状態の改善2016

    • Author(s)
      坂下惠一郎, 前田憲志, 吉崎重仁, 飯田俊郎, 寺田知新, 惠良聖一, 有川 一
    • Organizer
      一般社団法人日本分子状水素医学生物学会設立記念大会(第六回年会)
    • Place of Presentation
      横浜
    • Year and Date
      2016-05-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] Human cell lines of leukocytic, erythrocytic and megakaryocytic lineages affect redox state of human serum albumin2016

    • Author(s)
      Terada T, Takahashi T, Arikawa H, Era S
    • Organizer
      The 93rd Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Comparative study on the oxidative modification of recombinant and plasma-derived human serum albumin: thiol oxidation and carbonylation2016

    • Author(s)
      Takahashi T, Terada T, Arikawa H, Era S
    • Organizer
      The 93rd Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Effects of vocalization to physical activities and circulatory states during kendo exercises2016

    • Author(s)
      Arikawa H, Terada T, Takahashi T, Imai H, Era S
    • Organizer
      The 93rd Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Analysis of biological functions and protein structure in mutated FcεRI β chain (D234A)2015

    • Author(s)
      Terada T, Takahashi T, Arikawa H, Era S
    • Organizer
      8th Congress Federation of the Asian and Oceanian Physiological Societies
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Comparative study on the redox state of HSA products for laboratory use and healthe young subjects2015

    • Author(s)
      Takahashi, T, Terada T, Arikawa H, Imai H, Era, S
    • Organizer
      8th Congress Federation of the Asian and Oceanian Physiological Societies
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Sustained vocalizations during kendo exercises suppress expiration of carbon dioxide2015

    • Author(s)
      Arikawa H, Terada T, Takahashi T, Imai H, Era, S
    • Organizer
      8th Congress Federation of the Asian and Oceanian Physiological Societies
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 輸液用アルブミン製剤のチオール酸化とカルボニル化の解析2015

    • Author(s)
      高橋哲平, 寺田知新, 有川一, 恵良聖一
    • Organizer
      第62回中部日本生理学会
    • Place of Presentation
      富山
    • Year and Date
      2015-11-13
    • Related Report
      2015 Research-status Report
  • [Presentation] Mutated FcεRI β chain (D234A) affects signal transduction of mast cell but does not affect protein structure and thermal stability of FcεRI β chain protein2015

    • Author(s)
      Terada T, Takahashi T, Arikawa H, Era S
    • Organizer
      The 92th Annual Meeting of the PSJ/The 120th Annual Meeting of the JAA
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Sustained vocalizations suppress expiration of carbon dioxide during kendo exercise2015

    • Author(s)
      Arikawa H, Terada T, Takahashi T, Kizaki K, Imai H, Era S
    • Organizer
      The 92th Annual Meeting of the PSJ/The 120th Annual Meeting of the JAA
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] 市販ヒト血清アルブミン製品の分子不均一性に関する研究2014

    • Author(s)
      南武志、寺田知新、高橋哲平、有川 一、松山幸枝、恵良聖一
    • Organizer
      第61回中部日本生理学会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-11-07 – 2014-11-08
    • Related Report
      2014 Research-status Report
  • [Presentation] 継続的な発声を伴う剣道実施は,実施者の呼気二酸化炭素排出を抑制する2014

    • Author(s)
      有川一、寺田知新、高橋哲平、木崎一葉、今井 一、恵良聖一
    • Organizer
      第61回中部日本生理学会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-11-07 – 2014-11-08
    • Related Report
      2014 Research-status Report
  • [Presentation] The Effect of Hydrogen-Enriched Dialysate on Redox State of Human Serum Albumin2014

    • Author(s)
      Terawaki H, Nakayama M, Zhu WJ, Kabayama S, Terada T, Takahashi T, Matsuyama Y, Sakurai K, Hayashi Y, Watanabe T, Era S
    • Organizer
      ISN NEXUS SYMPOSIUM
    • Place of Presentation
      Bergamo Italy
    • Year and Date
      2014-04-03 – 2014-04-06
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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