Identification of molecules associated with high affinity IgE Fc receptor beta chain D234 and its application to therapy and drug discovery

• Project/Area Number: 26461577
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Gifu University
• Principal Investigator: TERADA TOMOYOSHI 岐阜大学, 医学部, 准教授 (30345780)
• Co-Investigator(Kenkyū-buntansha): 恵良 聖一 岐阜大学, 大学院医学系研究科, 非常勤講師 (30152002)
• Co-Investigator(Renkei-kenkyūsha): TOMIDA Mihoko 松本歯科大学, 歯学部, 教授 (00366329)
• Research Collaborator: ARIKAWA Hajime ; TAKAHASHI Teppei
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 高親和性IgE受容体β鎖 / 生物物理 / シグナル伝達 / アレルギー・ぜんそく / 蛋白質

Outline of Final Research Achievements

The high affinity IgE Fc receptor (FcεRI) β chain is an important molecule for signal transduction of mast cells. We confirmed the secondary, tertiary structure and thermal stability of β chain mutant (D234A) protein in which the 234th amino acid of C-terminal β chain was substituted with alanine, which is suggested to be involved in mast cell signal transduction. In addition, it was revealed that the β chain polymorphism (E228G) protein affects thermal stability, although there is no difference in the secondary and tertiary structure as compared with the wild type. The structure and thermodynamic properties of the β chain clarified in this study are expected to be useful for drug discovery targeting the β chain.

Research Products

• [Journal Article] Improvement of the fraction of human mercaptalbumin on hemodialysis treatment using hydrogen-dissolved hemodialysis fluid: a prospective observational study (2016)
  • Author(s): Maeda K, Yoshizaki S, Iida T, Terada T, Era S, Sakashita K, Arikawa H
  • Journal Title: Renal Replacement Therapy Volume: 2 Issue: 1 Pages: 42-42
  • DOI: 10.1186/s41100-016-0054-y
  • Peer Reviewed / Open Access
• [Journal Article] Analysis of the conformation and thermal stability of the high-affinity IgE Fc receptor β chain polymorphic proteins (2016)
  • Author(s): Terada T, Takahashi T, Arikawa H, Era S
  • Journal Title: Biosci Biotechnol Biochem Volume: in press Issue: 7 Pages: 1356-1361
  • DOI: 10.1080/09168451.2016.1153958
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Quantitation of Oxidative Modifications of Commercial Human Albumin for Clinical Use (2016)
  • Author(s): Takahashi T, Terada T, Arikawa H, Kizaki K, Terawaki H, Imai H, Itoh Y, Era S
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 39 Issue: 3 Pages: 401-408
  • DOI: 10.1248/bpb.b15-00843
  • NAID: 130005132297
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed / Open Access
• [Journal Article] Effect of reduced coenzyme Q10 (ubiquinol) supplementation on blood pressure and muscle damage during kendo training camp: a double-blind, randomized controlled study (2015)
  • Author(s): Kizaki K, Terada T, Arikawa H, Tajima T, Imai H, Takahashi T, Era S
  • Journal Title: J Sports Med Phys Fitness Volume: 55(7-8) Pages: 797-804
  • Peer Reviewed
• [Journal Article] Continuous Vocalization during Kendo Exercises Suppresses Expiration Of CO2 (2015)
  • Author(s): Arikawa H, Terada T, Takahashi T, Kizaki K, Imai H, Era S
  • Journal Title: Int J Sports Med Volume: 36(7) Issue: 07 Pages: 519-525
  • DOI: 10.1055/s-0034-1398679
  • Peer Reviewed
• [Journal Article] Effect of reduced coenzyme Q10 (ubiquinol) supplementation on blood pressure and muscle damage during kendo training camp: a double-blind, randomized controlled study (2015)
  • Author(s): Kizaki K, Terada T, Arikawa H, Tajima T, Imai H, Takahashi T, Era S
  • Journal Title: J Sports Med Phys Fitness Volume: in press
  • Peer Reviewed
• [Journal Article] Comparative studies on the heterogeneity of plasma-derived and recombinant human albumins in laboratory use (2014)
  • Author(s): Minami T, Terada T, Takahashi T, Arikawa H, Matsuyama Y, Kizaki K, Era S
  • Journal Title: Int J Biol Macromol Volume: 69 Pages: 79-87
  • DOI: 10.1016/j.ijbiomac.2014.05.010
  • Peer Reviewed
• [Journal Article] Effect of a hydrogen (H2 )-enriched solution on the albumin redox of hemodialysis patients. (2014)
  • Author(s): Terawaki H, Zhu WJ, Matsuyama Y, Terada T, Takahashi Y, Sakurai K, Kabayama S, Miyazaki M, Itami N, Nakazawa R, Ito S, Era S, Nakayama M
  • Journal Title: Hemodial Int Volume: In Press Issue: 2 Pages: 459-466
  • DOI: 10.1111/hdi.12112
  • Peer Reviewed
• [Journal Article] 血漿アルブミンの還元化率を改善する還元薬剤の透析液への添加とその有効濃度 (2014)
  • Author(s): 鈴木祥史、須田健二、松山幸枝、寺田知新、恵良聖一、副島昭典
  • Journal Title: 腎とフリーラジカル Volume: 12 Pages: 66-70
  • Peer Reviewed
• [Presentation] 長期の水素溶存透析液による血液透析はアルブミンレドックスを改善する (2016)
  • Author(s): 坂下惠一郎, 前田憲志, 飯田俊郎, 吉崎重仁, 寺田知新, 惠良聖一
  • Organizer: 第７回腎不全研究会
  • Place of Presentation: 東京
  • Year and Date: 2016-12-10
• [Presentation] 電解水透析によるAlbumin redoxの改善～より長期の検討 (2016)
  • Author(s): 吉崎重仁, 近藤弥生, 加藤博愛, 平林雪江, 白川智栄, 中嶋俊介, 玉井 泉, 安藤律子, 稲田清美, 飯田俊郎, 前田憲志, 坂下惠一郎, 寺田知新, 惠良聖一
  • Organizer: 第93回東海透析研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2016-09-25
• [Presentation] Improvement of the albumin redox state on hemodialysis treatment using hydrogen-dissolved hemodialysis fluid (2016)
  • Author(s): Sakashita K, Maeda K, Yosizaki S, Toshiro Iida T, Terada T, Seiichi Era S, Arikawa H
  • Organizer: ISBP2016 34th Congress of International Society of Blood Purification
  • Place of Presentation: Hiroshima
  • Year and Date: 2016-09-08
  • Int'l Joint Research
• [Presentation] 水素溶存透析液を用いた血液透析治療におけるアルブミン酸化還元状態の改善 (2016)
  • Author(s): 坂下惠一郎, 前田憲志, 吉崎重仁, 飯田俊郎, 寺田知新, 惠良聖一, 有川 一
  • Organizer: 一般社団法人日本分子状水素医学生物学会設立記念大会（第六回年会）
  • Place of Presentation: 横浜
  • Year and Date: 2016-05-27
• [Presentation] Human cell lines of leukocytic, erythrocytic and megakaryocytic lineages affect redox state of human serum albumin (2016)
  • Author(s): Terada T, Takahashi T, Arikawa H, Era S
  • Organizer: The 93rd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 札幌
  • Year and Date: 2016-03-22
• [Presentation] Comparative study on the oxidative modification of recombinant and plasma-derived human serum albumin: thiol oxidation and carbonylation (2016)
  • Author(s): Takahashi T, Terada T, Arikawa H, Era S
  • Organizer: The 93rd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 札幌
  • Year and Date: 2016-03-22
• [Presentation] Effects of vocalization to physical activities and circulatory states during kendo exercises (2016)
  • Author(s): Arikawa H, Terada T, Takahashi T, Imai H, Era S
  • Organizer: The 93rd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 札幌
  • Year and Date: 2016-03-22
• [Presentation] Analysis of biological functions and protein structure in mutated FcεRI β chain (D234A) (2015)
  • Author(s): Terada T, Takahashi T, Arikawa H, Era S
  • Organizer: 8th Congress Federation of the Asian and Oceanian Physiological Societies
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2015-11-22
  • Int'l Joint Research
• [Presentation] Comparative study on the redox state of HSA products for laboratory use and healthe young subjects (2015)
  • Author(s): Takahashi, T, Terada T, Arikawa H, Imai H, Era, S
  • Organizer: 8th Congress Federation of the Asian and Oceanian Physiological Societies
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2015-11-22
  • Int'l Joint Research
• [Presentation] Sustained vocalizations during kendo exercises suppress expiration of carbon dioxide (2015)
  • Author(s): Arikawa H, Terada T, Takahashi T, Imai H, Era, S
  • Organizer: 8th Congress Federation of the Asian and Oceanian Physiological Societies
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2015-11-22
  • Int'l Joint Research
• [Presentation] 輸液用アルブミン製剤のチオール酸化とカルボニル化の解析 (2015)
  • Author(s): 高橋哲平, 寺田知新, 有川一, 恵良聖一
  • Organizer: 第62回中部日本生理学会
  • Place of Presentation: 富山
  • Year and Date: 2015-11-13
• [Presentation] Mutated FcεRI β chain (D234A) affects signal transduction of mast cell but does not affect protein structure and thermal stability of FcεRI β chain protein (2015)
  • Author(s): Terada T, Takahashi T, Arikawa H, Era S
  • Organizer: The 92th Annual Meeting of the PSJ/The 120th Annual Meeting of the JAA
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Sustained vocalizations suppress expiration of carbon dioxide during kendo exercise (2015)
  • Author(s): Arikawa H, Terada T, Takahashi T, Kizaki K, Imai H, Era S
  • Organizer: The 92th Annual Meeting of the PSJ/The 120th Annual Meeting of the JAA
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] 市販ヒト血清アルブミン製品の分子不均一性に関する研究 (2014)
  • Author(s): 南武志、寺田知新、高橋哲平、有川 一、松山幸枝、恵良聖一
  • Organizer: 第61回中部日本生理学会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-11-07 – 2014-11-08
• [Presentation] 継続的な発声を伴う剣道実施は,実施者の呼気二酸化炭素排出を抑制する (2014)
  • Author(s): 有川一、寺田知新、高橋哲平、木崎一葉、今井 一、恵良聖一
  • Organizer: 第61回中部日本生理学会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-11-07 – 2014-11-08
• [Presentation] The Effect of Hydrogen-Enriched Dialysate on Redox State of Human Serum Albumin (2014)
  • Author(s): Terawaki H, Nakayama M, Zhu WJ, Kabayama S, Terada T, Takahashi T, Matsuyama Y, Sakurai K, Hayashi Y, Watanabe T, Era S
  • Organizer: ISN NEXUS SYMPOSIUM
  • Place of Presentation: Bergamo Italy
  • Year and Date: 2014-04-03 – 2014-04-06