Leukemogenic pathway of TEL-AML1-positive leukemia
Project/Area Number |
26461587
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pediatrics
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Research Institution | Ehime University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
石井 榮一 愛媛大学, 医学系研究科, 教授 (20176126)
江口 真理子 愛媛大学, 医学系研究科, 准教授 (40420781)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 白血病 / 融合遺伝子 / TEL-AML1融合遺伝子 / 付加的遺伝子異常 / 小児血液学 |
Outline of Final Research Achievements |
TEL-AML1 is the most common fusion gene in childhood acute lymphoblastic leukemia (ALL). It alone is not enough to cause leukemia, and additional genetic change(s) are necessary. Retroviral vector-mediated insertional mutagenesis was applied on TEL-AML1-expressing hematopoietic progenitor cells to identify the cooperating genetic changes with TEL-AML1 in leukemogenesis. Tie2-positive cells containing immature hematopoietic progenitors were transduced by retroviral vector to induce insertional mutagenesis, and then transplanted into immunodeficient mice. In these mice B220-positive cells were expanded, and tumor formation was observed in some of them. Analysis of retroviral integration site in these tumors showed intra-genic insertion of viral sequences possibly resulting in disruption of gene expression/regulation. Genes located near the integration site may be the target of cooperating genetic changes necessary for leukemogenesis by TEL-AML1.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Installation of multiple automated external defibrillators to prevent sudden death in school-aged children.2016
Author(s)
Higaki T., Chisaka T, Moritani T, Ohta M, Takata H, Yamauchi T, Yamaguchi Y, Konishi K, Yamamoto E, Ochi F, Eguchi M, Eguchi-Ishimae M, Mitani Y, Ishii E
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Journal Title
Pediatr Int
Volume: 58
Issue: 12
Pages: 1261-1265
DOI
Related Report
Peer Reviewed
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