| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Outline of Final Research Achievements |
TEL-AML1 is the most common fusion gene in childhood acute lymphoblastic leukemia (ALL). It alone is not enough to cause leukemia, and additional genetic change(s) are necessary. Retroviral vector-mediated insertional mutagenesis was applied on TEL-AML1-expressing hematopoietic progenitor cells to identify the cooperating genetic changes with TEL-AML1 in leukemogenesis. Tie2-positive cells containing immature hematopoietic progenitors were transduced by retroviral vector to induce insertional mutagenesis, and then transplanted into immunodeficient mice. In these mice B220-positive cells were expanded, and tumor formation was observed in some of them. Analysis of retroviral integration site in these tumors showed intra-genic insertion of viral sequences possibly resulting in disruption of gene expression/regulation. Genes located near the integration site may be the target of cooperating genetic changes necessary for leukemogenesis by TEL-AML1.
|
