Transmission of virulence and antibiotic resistance genes among Enteroaggregative Escherichia coli strains isolated from children

• Project/Area Number: 26461589
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kagoshima University
• Principal Investigator: Nishi Junichiro 鹿児島大学, 医歯学域医学系, 教授 (40295241)
• Co-Investigator(Kenkyū-buntansha): 藺牟田 直子 鹿児島大学, 医歯学域医学系, 助教 (00643470) ; 大岡 唯祐 鹿児島大学, 医歯学域医学系, 講師 (50363594)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: Ｅｓｃｈｅｒｉｃｈｉa coli / EAEC / ESBL / K1 capsule / 基質拡張型βラクタマーゼ（ESBL） / 大腸菌 / K1莢膜 / 腸管凝集性大腸菌 / 腸管病原性大腸菌 / 下痢原性大腸菌 / 尿路病原性大腸菌 / 基質拡張型βラクタマーゼ / MLSA / ゲノム解析 / 遺伝的多様性

Outline of Final Research Achievements

We performed phylogenetic analysis of entereaggregative Escherichia coli (EAEC) 167 strains isolated in Japan and found five major clones including an EAEC/UPEC (uropathogencic E. coli) hybrid clone O25:H4/ST131 possessing extend-spectrum beta-lactamase (ESBL) blaCTX-M gene. The incidence of blaCTX-M-positive strains among E. coli isolates from diarrheal children increases since 2013, and reached to 10.7% in 2013. Pathogenic genes among blaCTX-M-positive E. coli strains were as follows: EAEC aggR, 19%; EPEC (enteropathogenic E. coli) eae, 0.01%; K1 capsule gene neuC, 15.1%. These results suggest the pronounced horizontal transfer of blaCTX-M and pathogenic genes.

Research Products

• [Journal Article] Detection of virulence genes, phylogenetic group and antibiotic resistance of uropathogenic Escherichia coli in Mongolia. (2017)
  • Author(s): Munkhdelger Y, Gunregjav N, Dorjpurev A, Junichiro Nishi,Sarantuya J
  • Journal Title: J Infect Dev Ctries Volume: 11（1） Issue: 01 Pages: 51-57
  • DOI: 10.3855/jidc.7903
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Phylogenetic analysis of enteroaggregative Escherichia coli (EAEC) isolates from Japan reveals emergence of CTX-M-14-producing EAEC O25:H4 clones related to sequence type 131. (2016)
  • Author(s): Imuta N, Ooka T, Seto K, Kawahara R, Koriyama T, Kojyo T, Iguchi A, Tokuda K, Kawamura H, Yoshiie K, Ogura Y, Hayashi T, Nishi J
  • Journal Title: J Clin Microbiol Volume: 54（8） Issue: 8 Pages: 2128-2134
  • DOI: 10.1128/jcm.00711-16
  • Peer Reviewed / Open Access
• [Journal Article] Genes essential for the morphogenesis of the Shiga toxin 2-transducing phage from Escherichia coli O157:H7 (2016)
  • Author(s): Mondal SI, Islam MR, Sawaguchi A, Asadulghani M, Ooka T, Gotoh Y, Kasahara Y, Ogura Y, Hayashi T.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 39036-39036
  • DOI: 10.1038/srep39036
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A role for fosfomycin treatment in children for prevention of hemolytic uremic syndrome accompanying Shiga toxin-producing Escherichia coli infection (2015)
  • Author(s): Tajiri H, Nishi J, Ushijima K, Shimizu T, Ishige T, Shimizu M, Tanaka H, Brooks S.
  • Journal Title: Int J Antimicrob Agents Volume: 46（5） Issue: 5 Pages: 586-589
  • DOI: 10.1016/j.ijantimicag.2015.08.006
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Defining the Genome Features of Escherichia albertii, an Emerging Enteropathogen Closely Related to Escherichia coli (2015)
  • Author(s): Ooka T, Ogura Y, Katsura K, Seto K, Kobayashi H, Kawano K, Tokuoka E, Furukawa M, Harada S, Yoshino S, Seto J, Ikeda T, Yamaguchi K, Murase K, Gotoh Y, Imuta N, Nishi J, Gomes TA, Beutin L, Hayashi T.
  • Journal Title: Genome Biol Evol Volume: 7(12) Pages: 3170-3179
  • DOI: 10.1093/gbe/evv211
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 小児の便由来大腸菌におけるESBL産生K1莢膜保有株の割合 (2016)
  • Author(s): 藺牟田直子、徳田浩一、西 順一郎
  • Organizer: 第48回日本小児感染症学会総会
  • Place of Presentation: 岡山コンベンションセンター（岡山県岡山市）
  • Year and Date: 2016-10-19
• [Presentation] Variation of biofilm forming ability of aggR-positive Escherichia coli strains (2016)
  • Author(s): Imuta N, Ooka, Yoshiie K, Nishi J
  • Organizer: The 116th General Meeting of the American Society for Microbiology
  • Place of Presentation: Boston, USA
  • Year and Date: 2016-06-16
  • Int'l Joint Research
• [Presentation] 下痢症患児由来大腸菌におけるESBL（CTX-M）遺伝子保有頻度の年次推移 (2016)
  • Author(s): 藺牟田直子、川村英樹、徳田浩一、西 順一郎
  • Organizer: 第64回日本化学療法学会総会
  • Place of Presentation: 神戸国際展示場・神戸ポートピアホテル（兵庫県神戸市）
  • Year and Date: 2016-06-09
• [Presentation] 下痢症患児由来大腸菌におけるK1莢膜遺伝子の分布 (2016)
  • Author(s): 藺牟田直子、大岡唯祐、古城 剛、郡山豊泰、徳田浩一、川村英樹、吉家清貴、西 順一郎
  • Organizer: 第90回日本感染症学会総会・学術講演会
  • Place of Presentation: 仙台国際センター・新展示施設（宮城県仙台市）
  • Year and Date: 2016-04-15
• [Presentation] 下痢症患児由来大腸菌おける腸管凝集性大腸菌EAEC とESBL産生大腸菌の動向（2011～2013) (2016)
  • Author(s): 藺牟田直子、大岡唯祐、吉家清貴、西 順一郎
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター（大阪市）
  • Year and Date: 2016-03-23
• [Presentation] ESBL (CTX-M)遺伝子を保有する腸管凝集性大腸菌EAEC O111の出現 (2015)
  • Author(s): 藺牟田直子、大岡唯祐、吉家清貴、西 順一郎
  • Organizer: 第85回日本感染症学会西日本地方会・第58回日本感染症学会中日本地方会学術集会・第63回日本化学療法学会西日本支部総会合同開催
  • Place of Presentation: 奈良春日野国際フォーラム（奈良市）
  • Year and Date: 2015-10-15
• [Presentation] 下痢症患児由来大腸菌おける腸管凝集性大腸菌EAEC とESBL産生大腸菌の動向（2011～2013)－EAEC O111・O127のCTX-M遺伝子獲得－ (2015)
  • Author(s): 藺牟田直子、大岡唯祐、吉家清貴、西 順一郎
  • Organizer: 第６８回細菌学会九州支部総会
  • Place of Presentation: ビーコンプラザ（別府市）
  • Year and Date: 2015-09-04
• [Presentation] 下痢症患児由来大腸菌おけるESBL産生大腸菌と腸管凝集性大腸菌EAECの動向（2011~2013)－EAEC O111のCTX-M遺伝子獲得－ (2015)
  • Author(s): 藺牟田直子、大岡唯祐、西 順一郎
  • Organizer: 第19回腸管出血性大腸菌感染症研究会
  • Place of Presentation: 国立医薬品食品衛生研究所（東京都世田谷区）
  • Year and Date: 2015-07-09
• [Presentation] Genetic heterogeneity of AggR-positive enteroaggregative Escherichia coli (EAEC); emergence of EAEC/UPEC O25:H4:ST131 producing ESBL (2015)
  • Author(s): Imuta N, Ooka T, Seto K, Yoshiie K, Nishi J
  • Organizer: The 115th General Meeting of the American Society for Microbiology
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2015-06-01
  • Int'l Joint Research
• [Presentation] Genetic heterogeneity of AggR-positive enteroaggregative Escherichia coli (EAEC): emergence of EAEC/UPEC O25:H4:ST131 producing ESBL (2015)
  • Author(s): Imuta N, Ooka T, Seto K, Yoshiie K, Ogura Y, Hayashi T, Nishi J.
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 岐阜市（長良川国際会館）
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] ESBLを産生する腸管凝集性大腸菌（EAEC）の遺伝的多様性． (2014)
  • Author(s): 藺牟田直子、勢戸和子、田口真澄、郡山豊泰、吉家清貴、久保田知洋、西 順一郎
  • Organizer: 第84回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 岡山（ママカリフォーラム）
  • Year and Date: 2014-10-23 – 2014-10-25
• [Presentation] 下痢原性大腸菌の遺伝的多様性 (2014)
  • Author(s): 西 順一郎
  • Organizer: 第84回日本感染症学会西日本地方会学術集会 教育講演
  • Place of Presentation: 岡山市（ママカリフォーラム）
  • Year and Date: 2014-10-23 – 2014-10-25
  • Invited
• [Presentation] 下痢原性大腸菌の薬剤耐性化－ESBL産生腸管凝集性大腸菌（EAEC）－ (2014)
  • Author(s): 藺牟田直子、徳田 浩一、西 順一郎
  • Organizer: 第46回日本小児感染症学会総会・学術集会
  • Place of Presentation: 東京（京王プラザホテル）
  • Year and Date: 2014-10-18 – 2014-10-19
• [Presentation] 腸管凝集性大腸菌(EAEC)の遺伝的多様性 (2014)
  • Author(s): 藺牟田直子、大岡唯祐、勢戸和子、徳田浩一、吉家清貴、小椋義俊、林 哲也、西 順一郎
  • Organizer: 第 67 回日本細菌学会九州支部総会
  • Place of Presentation: 鹿児島市（城山観光ホテル）
  • Year and Date: 2014-09-05 – 2014-09-06
• [Presentation] ESBL産生腸管凝集性大腸菌(EAEC)の遺伝的多様性 (2014)
  • Author(s): 藺牟田直子、大岡唯祐、勢戸和子、田口真澄、小椋義俊、林 哲也、西 順一郎
  • Organizer: 第18回腸管出血性大腸菌感染症研究会
  • Place of Presentation: 京都市（同志社大学寒梅館）
  • Year and Date: 2014-07-15 – 2014-07-16
• [Presentation] Molecular epidemiological analysis of extended-spectrum beta-lactamase (ESBL)-producing enteroaggregative Escherichia coli (EAEC) (2014)
  • Author(s): Nishi J, Imuta N, Tokuda K, Yoshiie K
  • Organizer: The 114th General Meeting of the American Society for Microbiology
  • Place of Presentation: Boston, USA
  • Year and Date: 2014-05-17 – 2014-05-20