The study of the prognostic factor search in pediatric acute myeloid leukemia using the next generation sequencing and MLPA method

• Project/Area Number: 26461599
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: National Center for Child Health and Development (2015-2016); Gunma Institute of Public Health and Environmental Sciences (2014)
• Principal Investigator: Ohki Kentaro 国立研究開発法人国立成育医療研究センター, 小児血液・腫瘍研究部, 室長 (50400966)
• Co-Investigator(Kenkyū-buntansha): 林 泰秀 群馬県衛生環境研究所, その他部局等, 研究員 (30238133) ; 朴 明子 群馬県衛生環境研究所, その他部局等, 研究員 (50450375) ; 外松 学 群馬県衛生環境研究所, その他部局等, 研究員 (70251113)
• Research Collaborator: HARA YUSUKE 群馬大学, 小児科 ; YOSHIDA KENICHI 京都大学
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 遺伝子 / 小児がん / 急性骨髄性白血病 / 次世代シーケンス解析 / MLPA解析 / 次世代シークエンス解析 / 臨床 / 急性骨髄性白血病（AML） / ゲノム / マイクロアレイ / 癌

Outline of Final Research Achievements

Acute myeloid leukemia (AML) is a molecularly and clinically heterogeneous disease. In this study, whole-exome sequencing (WES) of 63 pediatric AML patients revealed mutations in components of the cohesin complex (RAD21 and SMC3), BCORL1 and ASXL2 in addition to previously known gene mutations. We also revealed intratumoural heterogeneities in many patients, implicating multiple clonal evolution events in the development of AML.
Whole-transcriptome sequencing (WTS) and real-time polymerase chain reaction of pediatric de novo AML patients revealed PRDM16 gene overexpression. The overall survival (OS) and event-free survival (EFS) among PRDM16- overexpressing patients were significantly worse than in patients with low PRDM16 expression (3-year OS: 51% vs. 81%, p<0.001, 3-year EFS: 32% vs. 64%, p <0.001) irrespective of other cytogenetic alterations except for NPM1.

Research Products

• [Journal Article] ZNF384-related fusion genes consist of a subgroup with a characteristic immunophenotype in childhood B-cell precursor acute lymphoblastic leukemia. (2017)
  • Author(s): Hirabayashi S, Ohki K, Nakabayashi K, Ichikawa H, Momozawa Y, Okamura K, Yaguchi A, Terada K, Saito Y, Yoshimi A, Ogata-Kawata H, Sakamoto H, Kato M, Fujimura J, Hino M, Kinoshita A, Kakuda H, Kurosawa H, Kato K, Kajiwara R, Moriwaki K, Morimoto T, Nakamura K, Noguchi Y, Osumi T, Sakashita K, Takita J, et al.
  • Journal Title: Haematologica. Volume: 102 Issue: 1 Pages: 118-129
  • DOI: 10.3324/haematol.2016.151035
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prognostic impact of specific molecular profiles in pediatric acute megakaryoblastic leukemia in non-Down syndrome. (2017)
  • Author(s): Hara Y, Shiba N, Ohki K, Tabuchi K, Yamato G, Park MJ, Tomizawa D, Kinoshita K, Shimada A, Arakawa H, Saito AM, Kiyokawa N, Tawa A, Horibe K, Taga T, Adachi S, Taki T, and Hayashi Y.
  • Journal Title: Genes, Chromosomes & Cancer Volume: 14 February 2017 Issue: 5 Pages: 394-404
  • DOI: 10.1002/gcc.22444
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] ASXL2 Mutations are Frequently Found in Pediatric AML Patients with t(8;21)/RUNX1-RUNX1T1 and Associated with a Better Prognosis. (2017)
  • Author(s): Yamato G, Shiba N, Yoshida K, Shiraishi Y, Hara Y, Ohki K, Okubo J, Okuno H, Chiba K, Tanaka H, Kinoshita A, Moritake H, Kiyokawa N, Tomizawa D, Park MJ, Sotomatsu M, Taga T, Adachi S, Tawa A, Horibe K, Arakawa H, Miyano S, Ogawa S, Hayashi Y.
  • Journal Title: Genes Chromosomes Cancer. Volume: - Issue: 5 Pages: 382-393
  • DOI: 10.1002/gcc.22443
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Whole-exome sequencing reveals the spectrum of gene mutations and the clonal evolution patterns in paediatric acute myeloid leukaemia. (2016)
  • Author(s): Shiba N, Yoshida K, Shiraishi Y, Okuno Y, Yamato G, Hara Y, Nagata Y, Chiba K, Tanaka H, Terui K, Kato M, Park MJ, Ohki K, Shimada A, Takita J, Tomizawa D, Kudo K, Arakawa H, Adachi S, Taga T, Tawa A, Ito E, Horibe K, Sanada M, Miyano S, Ogawa S, Hayashi Y.
  • Journal Title: Br J Haematol. Volume: 175 Issue: 3 Pages: 476-489
  • DOI: 10.1111/bjh.14247
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ph-like ALL-related novel fusion kinase ATF7IP&#8211;PDGFRB exhibits high sensitivity to tyrosine kinase inhibitors in murine cells. (2016)
  • Author(s): Takeshi Ishibashi, Akinori Yaguchi, Kazuki Terada, Hitomi Ueno-Yokohata, Osamu Tomita, Kazutoshi Iijima, Kenichiro Kobayashi, Hajime Okita, Junya Fujimura, Kentaro Ohki, Toshiaki Shimizu, Nobutaka Kiyokawa.
  • Journal Title: Experimental Hematology. Volume: 44 Issue: 3 Pages: 177-188
  • DOI: 10.1016/j.exphem.2015.11.009
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] High PRDM16 expression identifies a prognostic subgroup of pediatric acute myeloid leukaemia correlated to FLT3-ITD, KMT2A-PTD, and NUP98-NSD1: the results of the Japanese Paediatric Leukaemia/Lymphoma Study Group AML-05 trial. (2015)
  • Author(s): Shiba N, Ohki K, Kobayashi T, Hara Y, Yamato G, Tanoshima R, Ichikawa H, Tomizawa D, Park MJ, Shimada A, Sotomatsu M, Arakawa H, Horibe K, Adachi S, Taga T, Tawa A, Hayashi Y.
  • Journal Title: Br J Haematol. Volume: 172 Issue: 4 Pages: 581-91
  • DOI: 10.1111/bjh.13869
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 小児急性骨髄性白血病における全エクソン解析による新規原因遺伝子の同定 (2015)
  • Author(s): 柴 徳生
  • Journal Title: 臨床血液 Volume: 52 Pages: 2419-2425
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] High expression of EVI1 and MEL1 is a compelling poor prognostic marker of pediatric AML. (2015)
  • Author(s): Jo A, Mitani S, Shiba N, Hayashi Y, Hara Y, Takahashi H, Tsukimoto I, Tawa A, Horibe K, Tomizawa D, Taga T, Adachi S, Yoshida T, Ichikawa H
  • Journal Title: Leukemia Volume: 29 Issue: 5 Pages: 1067-1083
  • DOI: 10.1038/leu.2015.5
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] CSF3R and CALR mutations in paediatric myeloid disorders and the association of CSF3R mutations with translocations, including t(8; 21). (2015)
  • Author(s): Sano H, Ohki K, Park MJ, Shiba N, et al.
  • Journal Title: Br J Haematol. Volume: Epub ahead of print Issue: 3 Pages: 319-397
  • DOI: 10.1111/bjh.13439
  • Peer Reviewed / Open Access
• [Journal Article] Adverse prognostic impact of KIT mutations in childhood CBF-AML: the results of the Japanese Pediatric Leukemia/Lymphoma Study Group AML-05 trial. (2015)
  • Author(s): Tokumasu M, Murata C, Shimada A, Ohki K, Hayashi Y, Saito AM, Fujimoto J, Horibe K, Nagao M, Itoh H, Kamikubo Y, Nakayama H, Kinoshita A, Tomizawa D, Taga T, Tawa A, Tanaka S, Heike T, Adachi S
  • Journal Title: Leukemia Volume: 29 Issue: 12 Pages: 2438-2441
  • DOI: 10.1038/leu.2015.121
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Early use of allogeneic hematopoietic stem cell transplantation for infants with MLL gene-rearrangement-positive acute lymphoblastic leukemia. (2015)
  • Author(s): Koh K, Tomizawa D, Moriya Saito A, Watanabe T, Miyamura T, Hirayama M, Takahashi Y, Ogawa A, Kato K, Sugita K, Sato T, Deguchi T, Hayashi Y, Takita J, Takeshita Y, Tsurusawa M, Horibe K, Mizutani S, Ishii E.
  • Journal Title: Leukemia Volume: 29(2) Issue: 2 Pages: 290-6
  • DOI: 10.1038/leu.2014.172
  • Peer Reviewed / Open Access
• [Journal Article] 小児Ph-like ALL症例の表面マーカー、遺伝子発現解析. (2014)
  • Author(s): 飯島 一智, 清河 信敬, 吉原 宏樹, 富田 理, 小林 健一郎, 福島 敬, 林 泰秀, 菊地 陽, 康 勝好, 真部 淳, 小原 明.
  • Journal Title: 日本小児血液・がん学会雑誌 Volume: 51 Pages: 200-205
  • Peer Reviewed
• [Journal Article] Gene alterations involving the CRLF2-JAK pathway and recurrent gene deletions in Down syndrome-associated acute lymphoblastic leukemia in Japan. (2014)
  • Author(s): Hanada I, Terui K, Ikeda F, Toki T, Kanezaki R, Sato T, Kamio T, Kudo K, Sasaki S, Takahashi Y, Hayashi Y, Inukai T, Kojima S, Koike K, Kosaka Y, Kobayashi M, Imaizumi M, Mitsui T, Hori H, Hara J, Horibe K, Nagai J, Goto H, Ito E.
  • Journal Title: Genes Chromosomes Cancer Volume: 53 Issue: 11 Pages: 902-910
  • DOI: 10.1002/gcc.22201
  • Peer Reviewed / Open Access
• [Journal Article] Prognostic impact of gained chromosomes in high-hyperdiploid childhood acute lymphoblastic leukaemia (2014)
  • Author(s): Kato M, Imamura T, Manabe A, Hashii Y, Koh K, Sato A, Takahashi H, Hori H, Taki T, Inoue M, Hayashi Y, Horibe K, Tsuchida M, Kojima S, Oda M, Ohara A .
  • Journal Title: Br J Haematol Volume: 166 Issue: 2 Pages: 295-298
  • DOI: 10.1111/bjh.12836
  • Peer Reviewed
• [Journal Article] Acute myeloid leukaemia with myelodysplastic features in children (2014)
  • Author(s): Kinoshita A, Miyachi H, Matsushita H, Yabe M, Taki T, Watanabe T, Saito AM, Tomizawa D, Taga T, Takahashi H, Matsuo H, Kodama K, Ohki K, Hayashi Y, Tawa A, Horibe K, Adachi S.
  • Journal Title: Br J Haematol Volume: 167 Issue: 1 Pages: 80-86
  • DOI: 10.1111/bjh.12993
  • Peer Reviewed
• [Journal Article] Significance of CD66c expression in childhood acute lymphoblastic leukemia. (2014)
  • Author(s): Kiyokawa N, Iijima K, Tomita O, Miharu M, Hasegawa D, Kobayashi K, Okita H, Kajiwara M, Shimada H, Inukai T, Makimoto A, Fukushima T, Nanmoku T, Koh K, Manabe A, Kikuchi A, Sugita K, Fujimoto J, Hayashi Y, Ohara A.
  • Journal Title: Leuk Res. Volume: 38 Issue: 1 Pages: 42-8
  • DOI: 10.1016/j.leukres.2013.10.008
  • Peer Reviewed
• [Journal Article] EVI1 overexpression is a poor prognostic factor in pediatric patients with mixed lineage leukemia-AF9 rearranged acute myeloid leukemia. (2014)
  • Author(s): Matsuo H, Kajihara M, Tomizawa D, Watanabe T, Saito AM, Fujimoto J, Horibe K, Kodama K, Tokumasu M, Itoh H, Nakayama H, Kinoshita A, Taga T, Tawa A, Taki T, Shiba N, Ohki K, Hayashi Y, Yamashita Y, Shimada A, Tanaka S, Adachi S.
  • Journal Title: Haematologica Volume: 99 Issue: 11 Pages: 225-227
  • DOI: 10.3324/haematol.2014.107128
  • Peer Reviewed / Open Access
• [Journal Article] Liver disease is frequently observed in Down syndrome patients with transient abnormal myelopoiesis. (2014)
  • Author(s): Park MJ, Sotomatsu M, Ohki K, Arai K, Maruyama K, Kobayashi T, Nishi A, Sameshima K, Takagi T, Hayashi Y.
  • Journal Title: Int J Hematol Volume: 99 Issue: 2 Pages: 154-161
  • DOI: 10.1007/s12185-013-1487-5
  • Peer Reviewed
• [Journal Article] Mutations of the GATA2 and CEBPA genes in paediatric acute myeloid leukaemia. (2014)
  • Author(s): Shiba N, Funato M, Ohki K, Park MJ, Mizushima Y, Adachi S, Kobayashi M, Kinoshita A, Sotomatsu M, Arakawa H, Tawa A, Horibe K, Tsukimoto I, Hayashi Y.
  • Journal Title: Brit J Haematol Volume: 印刷中 Issue: 1 Pages: 142-159
  • DOI: 10.1111/bjh.12559
  • Peer Reviewed
• [Journal Article] Prognostic significance of leukopenia in childhood acute lymphoblastic leukemia. (2014)
  • Author(s): Shiozawa Y, Takita J, Kato M, Sotomatsu M, Koh K, Ida K, Hayashi Y.
  • Journal Title: Oncol Lett. Volume: 7 Pages: 1169-1174
  • Peer Reviewed
• [Journal Article] Genome-wide approach to identify second gene targets for malignant rhabdoid tumors using high-density oligonucleotide microarrays (2014)
  • Author(s): Takita J, Chen Y, Kato M, Ohki K, Sato Y, Ohta S, Sugita K, Nishimura R, Hoshino N, Seki M, Sanada M, Oka A, Hayashi Y, Ogawa S
  • Journal Title: Cancer Sci Volume: 105 Issue: 3 Pages: 258-64
  • DOI: 10.1111/cas.12352
  • Peer Reviewed / Open Access
• [Journal Article] SETBP1 mutations in juvenile myelomonocytic leukaemia and myelodysplastic syndrome but not in paediatric acute myeloid leukaemia (2013)
  • Author(s): Shiba N, Ohki K, Park MJ, Sotomatsu M, Kudo K, Ito E, Sako M, Arakawa H, Hayashi Y
  • Journal Title: Br J Haematol Volume: Epub ahead of print Issue: 1 Pages: 156-159
  • DOI: 10.1111/bjh.12595
  • Peer Reviewed
• [Presentation] Incidence and clinical relevance of MEF2D- and ZNF384-relattsuyoshied fusion genes in childhood B-cell precursor acute lymphoblastic leukemia: TCCSG L0416/0616 study (2016)
  • Author(s): 大木健太郎, 平林真介, 加藤元博, 中林一彦, 秦健一郎, 梶原良介, 高橋浩之, 福島敬, 康勝好, 真部淳, 小原明, 清河信敬.
  • Organizer: 第58回日本小児血液・がん学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2016-12-15
• [Presentation] Frequency and clinical features of RUNX1 mutations in pediatric acute myeloid leukemia patients. (2016)
  • Author(s): 大和玄季, 柴徳生, 吉田健一, 原勇介, 大木健太郎, 大久保淳, 朴明子, 外松学, 清河信敬, 富澤大輔, 多賀崇, 足立壮一, 多和昭雄, 堀部敬三, 荒川浩一, 小川誠司, 林泰秀
  • Organizer: 第58回日本小児血液・がん学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2016-12-15
• [Presentation] Novel risk stratification of infant acute myeloid leukemia using MLL-rearrangements and CBFA2T3-GLIS2. (2016)
  • Author(s): 原勇介, 柴徳生, 大木健太郎, 大和玄季, 朴明子, 富澤大輔, 滝智彦, 木下明俊, 清河信敬, 荒川浩一, 多和昭雄, 堀部敬三, 多賀崇, 足立壮一, 林泰秀
  • Organizer: 第58回日本小児血液・がん学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2016-12-15
• [Presentation] Genetic Abnormalities and Prognosis in Pediatric B-precursor Acute Lymphoblastic Leukemia without conventional genetic abnormalities; Tokyo Children’s Cancer Study Group. (2016)
  • Author(s): Ohki K, Hirabayashi S, Yaguchi A, Terada K, Ohkubo J, Shiba N, Kato M, Fukushima T, Koh K, Hayashi Y, Manabe A, Ohara A, Kiyokawa N.
  • Organizer: 58th ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] Clusters of Childhood Mixed Phenotype Acute Leukemia Diagnosed By Nation-Wide Immunophenotyping in Japan. (2016)
  • Author(s): Deguchi T, Kiyokawa N, Ohki K, Hashii Y, Manabe A, Adachi S, Saito AM, Horibe K, Komada Y.
  • Organizer: 58th ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] Retrospective Evaluation of Correlations Between Genetic Backgrounds and Stem Cell Transplantation for De Novo Pediatric Acute Myeloid Leukemia: A Study from the Japan Pediatric Leukemia/Lymphoma Study Group (JPLSG) AML-05 Clinical Trial. (2016)
  • Author(s): Ueno T, Yamato G, Shiba N, Hara Y, Ohki K, Ichikawa H, Park MJ, Shimada A, Sotomatsu M, Arakawa H, Horibe K, Adachi S, Taga T, Tawa A, Hayashi Y.
  • Organizer: 58th ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] Identification of Two Distinct Poor Prognostic Subgroups Related to High Expression of BMP2 or PRDM16 in Pediatric AML. (2016)
  • Author(s): Hara Y, Shiba N, Yamato G, Okubo J, Ohki K, Park MJ, Sotomatsu M, Tomizawa D, Taki T, Kinoshita A, Kiyokawa N, Taga T, Arakawa H, Tawa A, Horibe K, Adachi S, Hayashi Y.
  • Organizer: 58th ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] The Clinical Features and Prognostic Impact of PRDM16 gene Expression in Adult Acute Myeloid Leukemia. (2016)
  • Author(s): Yamato G, Yamaguchi H, Handa H, Shiba N, Wakita S, Inokuchi K, Hara Y, Ohki K, Okubo J, Sotomatsu M, Arakawa H, Hayashi Y.
  • Organizer: 58th ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] Transcriptome Analysis Revealed the Entire Genetic Understanding of Pediatric Acute Myeloid Leukemia with a Normal Karyotype. (2016)
  • Author(s): Shiba N, Yashida K, Shiraishi Y, Hara Y, Yamato G, Kaburagi T, Sanada M, Ohki K, Park MJ, Tomizawa D, Chiba K, Tanaka H, Sotomatsu M, Arakawa H, Horibe K, Taga T, Adachi S, Tawa A, Miyano S, Ogawa S, Hayashi Y.
  • Organizer: 58th ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] Transcriptome analysis of 92 pediatric acute myeloid leukemia patients. (2016)
  • Author(s): Norio Shiba, Kenichi Yoshida, Genki Yamato, Yusuke Hara, Masashi Sanada, Yuichi Shiraishi, Kentaro Ohki, Myoug-Ja Park, Taeko Kaburaki, Daisuke Tomizawa, Manabu Sotomatsu, Hirokazu Arakawa, Keizo Horibe, Takashi Taga, Souichi Adachi, Akio Tawa, Satoru Miyano, Seishi Ogawa, Yasuhida Hayashi
  • Organizer: The 78th Annual Meeting of the Japanese Society of Hematology
  • Place of Presentation: 横浜
  • Year and Date: 2016-10-13
• [Presentation] Molecular features and their clinical impact in infant acute myeloid leukemia (2016)
  • Author(s): Yusuke Hara, Norio Shiba, Kentaro Ohki, Genki Yamato, Myoug-Ja Park, Daisuke Tomizawa, Tomohiko Taki, Akitoshi Kinoshita, Hirokazu Arakawa, Akio Tawa, Keizo Horibe, Takashi Taga, Souichi Adachi, Yasuhide Hayashi
  • Organizer: The 78th Annual Meeting of the Japanese Society of Hematology
  • Place of Presentation: 横浜
  • Year and Date: 2016-10-13
• [Presentation] Clinical features of patients with ASXL1 and ASXL2 mutations in pediatric acute myeloid leukemia (2016)
  • Author(s): Genki Yamato, Norio Shiba, Kenichi Yoshida, Kentaro Ohki, Jun Okubo, Yusuke Hara, Akitoshi Kinoshita, Daisuke Tomizawa, Manabu Sotomatsu, Takashi Taga, Souichi Adachi, Akio Tawa, Keizo Horibe, Hirokazu Arakawa, Seishi Ogawa, Yasuhida Hayashi
  • Organizer: The 78th Annual Meeting of the Japanese Society of Hematology
  • Place of Presentation: 横浜
  • Year and Date: 2016-10-13
• [Presentation] A Combination of EVI1 and PRDM16 Expression Clarified the Clinical Features of Intermediate/High Risk Patients in Pediatric Acute Myeloid Leukemia. (2015)
  • Author(s): Yamato G, Shiba N, Hara Y, Park M, Sotomatsu M, Ohki K, Ichikawa H, Tomizawa D, Taga T, Horibe K, Tawa A, Arakawa H, Adachi S, Hayashi Y.
  • Organizer: 57th ASH Annual Meeting and Exposition
  • Place of Presentation: Orland
  • Year and Date: 2015-12-05
  • Int'l Joint Research
• [Presentation] Detection of Novel Pathogenic Gene Rearrangements in Pediatric Acute Myeloid Leukemia By RNA Sequencing. (2015)
  • Author(s): Shiba N, Yoshida K, Shiraishi Y, Hara Y, Yamato G, Kaburaki T, Sanada M, Oki K, Tomizawa D, Sotomatsu M, Arakawa H, Horibe K, Park M, Taga T, Adachi S, Tawa A, Miyano S, Ogawa S, Hayashi Y
  • Organizer: 57th ASH Annual Meeting and Exposition
  • Place of Presentation: Orland
  • Year and Date: 2015-12-05
  • Int'l Joint Research
• [Presentation] 小児T-ALLにおける6qLOHの頻度と予後解析 TCCSG ALL L0416/L0616. (2015)
  • Author(s): 大木 健太郎, 清河 信敬, 朴 明子, 小林 健一郎, 福島 敬, 康 勝好, 土田 昌宏, 林 泰秀, 真部 淳, 小原 明.
  • Organizer: 第57回日本小児血液・がん学会学術総会
  • Place of Presentation: 甲府
  • Year and Date: 2015-11-27
• [Presentation] RNA seqにより見いだされた新規遺伝子の解析と臨床像. (2015)
  • Author(s): 原 勇介, 柴 徳生, 大和 玄季, 大木 健太郎, 朴 明子, 外松 学, 滝 智彦, 吉田 健一, 眞田 昌, 富澤 大輔, 荒川 浩一, 堀部 敬三, 多賀 崇, 足立 壮一, 多和 昭雄, 宮野 悟, 小川 誠司, 林 泰秀
  • Organizer: 第57回日本小児血液・がん学会学術総会
  • Place of Presentation: 甲府
  • Year and Date: 2015-11-27
• [Presentation] 小児急性骨髄性白血病におけるPRDM16遺伝子とEVI1遺伝子発現の臨床的意義. (2015)
  • Author(s): 大和 玄季, 柴 徳生, 原 勇介, 朴 明子, 外松 学, 大木 健太郎, 市川 仁, 富澤 大輔, 多賀 崇, 堀部 敬三, 多和 昭雄, 荒川 浩一, 足立 壮一, 林 泰秀.
  • Organizer: 第57回日本小児血液・がん学会学術総会
  • Place of Presentation: 甲府
  • Year and Date: 2015-11-27
• [Presentation] Analysis of genes in pediatric B-precursor ALL treated at single facility. (2015)
  • Author(s): Ohki K, Arai K, Park M, Sotomatsu M, Hayashi Y.
  • Organizer: 第77回日本血液学会学術総会
  • Place of Presentation: 金沢
  • Year and Date: 2015-10-16
• [Presentation] The prognostic impact of PRDM16 gene expression in both adult and pediatric acute myeloid leukemia. (2015)
  • Author(s): Yamato G, Yamaguchi H, Handa H, Shiba N, Wakita S, Inokuchi K, Hara Y, Ohki K, Park M, Sotomatsu M, Ichikawa H, Tomizawa D, Taga T, Adachi S, Horibe K, Tawa A, Arakawa H, Hayashi Y.
  • Organizer: 第77回日本血液学会学術総会
  • Place of Presentation: 金沢
  • Year and Date: 2015-10-16
• [Presentation] RNA sequencing reveals the comprehensive gene alterations of pediatric acute myeloid leukemia. (2015)
  • Author(s): Shiba N, Yoshida K, Sanada M, Shiraishi Y, Hara Y, Yamato G, Ohki K, Park M, Tomizawa D, Sotomatsu M, Arakawa H, Horibe K, Taga T, Adachi S, Tawa A, Miyano S, Ogawa S, Hayashi Y.
  • Organizer: 第77回日本血液学会学術総会
  • Place of Presentation: 金沢
  • Year and Date: 2015-10-16
• [Presentation] 中間リスク、高リスク小児急性骨髄性白血病における新たな治療層別化の試み (2015)
  • Author(s): 柴 徳生, 原 勇介, 大木 健太郎, 大和 玄季, 富澤 大輔, 多賀 崇, 多和 昭雄, 荒川 浩一, 足立 壮一, 林 泰秀, JPLSG AML委員会
  • Organizer: 第118回日本小児科学会
  • Place of Presentation: 大阪
  • Year and Date: 2015-04-17
• [Presentation] Genetic Abnormalities and Prognosis in Pediatric B–precursor Acute Lymphoblastic Leukemia Treated on Tokyo Children′s Cancer Study Group Protocol. (2014)
  • Author(s): Ohki K, Park M, Hara Y, Shiba N, Fukushima T, Koh K, Manabe A, Ohara A, Kiyokawa N, Hayashi Y.
  • Organizer: 56th ASH Annual Meeting and Exposition
  • Place of Presentation: San Francisco
  • Year and Date: 2014-12-06 – 2014-12-09
• [Presentation] Differentiating Burkitt Lymphoma from Burkitt-like Lymphoma in Pediatrics: Report from the Japanese Pediatric Leukemia/Lymphoma Study Group B-NHL03 Study. (2014)
  • Author(s): Osumi T, Sekimizu M, Mori T, Fukano R, Koga Y, Ueyama J, Tanaka F, Ohki K, Mitsui T, Mori T, Sunami S, Kada A, Moriya Saito A, Horibe K, Koh K, Komada Y, Kosaka Y, Ohshima K, Nakazawa A, Tsurusawa M, Kobayashi R, Adachi S.
  • Organizer: 56th ASH Annual Meeting and Exposition
  • Place of Presentation: San Francisco
  • Year and Date: 2014-12-06 – 2014-12-09
• [Presentation] The Prognostic Impact of High MEL1 Gene Expression in Pediatric Acute Myeloid Leukemia. (2014)
  • Author(s): Shiba N, Ohki K, Hara Y, Yamato G, Park M, Ichikawa H, Kobayashi T, Tomizawa D, Sotomatsu M, Arakawa H, Horibe K, Taga T, Adachi S, Tawa A, Hayashi Y.
  • Organizer: 56th ASH Annual Meeting and Exposition
  • Place of Presentation: San Francisco
  • Year and Date: 2014-12-06 – 2014-12-09
• [Presentation] Poor Prognosis Associated with FAB Subtypes M4 and M5 in Japanese Pediatric Acute Myeloid Leukemia Patients with FLT3-ITD. (2014)
  • Author(s): Hara Y, Shiba N, Ohki K, Park M, Shimada A, Tomizawa D, Moriya Saito M, Fujimoto J, Taki T, Kinoshita A, Taga T, Arakawa H, Tawa A, Horibe K, Adachi S, Hayashi Y.
  • Organizer: 56th ASH Annual Meeting and Exposition
  • Place of Presentation: San Francisco
  • Year and Date: 2014-12-06 – 2014-12-09
• [Presentation] Clinical Features of Patients with ASXL1 and ASXL2 Mutations in Pediatric Acute Myeloid Leukemia. (2014)
  • Author(s): Yamato G, Shiba N, Yoshida K, Ohki K, Park M, Hara Y, Tomizawa D, Sotomatsu M, Taga T, Adachi S, Tawa A, Horibe K, Arakawa H, Ogawa S, Hayashi Y.
  • Organizer: 56th ASH Annual Meeting and Exposition
  • Place of Presentation: San Francisco
  • Year and Date: 2014-12-06 – 2014-12-09
• [Presentation] Whole Exome and Transcriptome Analyses in Pediatric T-Cell Acute Lymphoblastic Leukemia. (2014)
  • Author(s): Seki M, Yoshida K, Shiraishi Y, Chiba K, Tanaka H, Kato M, Hanada R, Miyano S, Hayashi Y, Ogawa S, Takita J.
  • Organizer: 56th ASH Annual Meeting and Exposition
  • Place of Presentation: San Francisco
  • Year and Date: 2014-12-06 – 2014-12-09
• [Presentation] 小児AMLにおけるIKZF1欠失の頻度と予後解析 JPLSG AML-05 (2014)
  • Author(s): 大木 健太郎, 朴 明子, 原 勇介, 柴 徳生, 外松 学, 富澤 大輔, 多賀 崇, 齋藤 明子, 藤本 純一郎, 多和 昭雄, 堀部 敬三, 足立 壮一, 林 泰秀.
  • Organizer: 第56回日本小児血液・がん学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2014-11-28 – 2014-11-30
• [Presentation] 小児急性骨髄性白血病における寛解導入療法後非寛解例の遺伝子解析による予後不良因子の同定 (2014)
  • Author(s): 原 勇介, 大木 健太郎, 柴 徳生, 朴 明子, 富澤 大輔, 多賀 崇, 荒川 浩一, 足立 壮一, 多和 昭雄, 林 泰秀.
  • Organizer: 第56回日本小児血液・がん学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2014-11-28 – 2014-11-30
• [Presentation] 小児急性骨髄性白血病におけるASXL1、ASXL2遺伝子変異と臨床像 (2014)
  • Author(s): 大和 玄季, 柴 徳生, 吉田 健一, 大木 健太郎, 朴 明子, 原 勇介, 外松 学, 多賀 崇, 富澤 大輔, 足立 壮一, 多和 昭雄, 堀部 敬三, 荒川 浩一, 小川 誠司, 林 泰秀.
  • Organizer: 第56回日本小児血液・がん学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2014-11-28 – 2014-11-30
• [Presentation] t(9;17)(p24;q21)をもつ思春期急性リンパ性白血病における新規JAK2融合遺伝子の同定 (2014)
  • Author(s): 川村 眞智子, 賀来 秀文, 滝 智彦, 大木 健太郎, 林 泰秀.
  • Organizer: 第56回日本小児血液・がん学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2014-11-28 – 2014-11-30
• [Presentation] Gene expression and DNA methylation related to prognosis of childhood ALL without fusion genes. (2014)
  • Author(s): Iijima K, Kiyokawa N, Ueno H, Yoshihara H, Hasegawa D, Ohki K, Kato M, Fukushima T, Koh K, Hayashi Y, Manabe A, Ohara A.
  • Organizer: 第76回日本血液学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] Analysis of iAMP21 and ERG genes in pediatric B-precursor ALL trated on TCCSG protocol.. (2014)
  • Author(s): Ohki K, Ohkita H, Kobayashi K, Shiba N, Park M, Sotomatsu M, Fukushima T, Koh K, Hanada R, Manabe A, Kikuchi A, Tsutida M, Ohara A, Kiyokawa N, Hayashi Y.
  • Organizer: 第76回日本血液学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] CSF3R and CALR mutations and cytogenetic findings in pediatric myeloid malignancies. (2014)
  • Author(s): Sano H, Ohki K, Park M, Shiba N, Hara Y, Sotomatsu M, Tomizawa D, Taga T, Kiyokawa N, Tawa A, Horibe K, Adachi A, Hayashi Y.
  • Organizer: 第76回日本血液学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] Genetic analysis of patients who did not achieve complete remission after induction therapy. (2014)
  • Author(s): Hara Y, Ohki K, Shiba N, Shimada A, Tomizawa A, Taga T, Adachi S, Arakawa H, Tawa A, Hayashi Y.
  • Organizer: 第76回日本血液学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] The prognostic impact of high EV1-related genes expression in pediatric acute myeloid leukemia. (2014)
  • Author(s): Shiba N, Hara Y, Ohki K, Yamato G, Park M, Kobayashi T, Ichikawa H, Tomizawa D, Taki T, Shimada A, Sotomatsu M, Arakawa H, Horibe H, Adachi S, Tawa A, Hayashi Y. The prognostic impact of high EV1-related genes expression in pediatric acute myeloid leukemia.
  • Organizer: 第76回日本血液学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] 小児急性骨髄性白血病における寛解導入療法終了後非寛解例の分子生物学的異常の同定と臨床像の検討. (2014)
  • Author(s): 原 勇介, 大木 健太郎, 柴 徳生, 朴 明子, 富澤 大輔, 多賀 崇, 足立 壮一, 荒川 浩一,多和 昭雄, 林 泰秀.
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] trisomy8とMLL-AF9を有する急性骨髄性白血病のclonal architectureの解析 (2014)
  • Author(s): 大木 健太郎, 鎌 裕一, 原 勇介, 佐野 仁志, 柴 徳生, 新井 心, 朴 明子, 外松 学, 林 泰秀.
  • Organizer: 第117回日本小児科学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-04-11 – 2014-04-13
• [Presentation] 小児AMLにおけるG-CSF receptor(CSF3R)遺伝子異常の解析. (2014)
  • Author(s): 佐野 仁志, 大木 健太郎, 朴 明子, 柴 徳生, 足立 壮一, 堀部 敬三, 多和 昭雄, 花田 良二, 月本 一郎, 林 泰秀.
  • Organizer: 第117回日本小児科学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-04-11 – 2014-04-13
• [Presentation] 小児急性骨髄性白血病におけるGATA2変異の解析. (2014)
  • Author(s): 原 勇介, 柴 徳生, 大木 健太郎, 朴 明子, 足立 壮一, 多賀 崇, 荒川 浩一, 多和 昭雄, 堀部 敬三, 林 泰秀.
  • Organizer: 第117回日本小児科学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-04-11 – 2014-04-13
• [Presentation] 次世代シークエンサーを用いた神経芽腫における11q領域の責任遺伝子探索. (2014)
  • Author(s): 吉田 美沙, 関 正史, 星野 論子, 樋渡 光輝, 加藤 元博, 吉田 健一, 小川 誠司, 林 泰秀, 滝田 順子, 岡 明.
  • Organizer: 第117回日本小児科学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-04-11 – 2014-04-13
• [Presentation] 小児急性骨髄性白血病における分子生物学的背景を用いた新たな治療層別化への試み. (2014)
  • Author(s): 柴 徳生, 吉田 健一, 大木 健太郎, 金澤 崇, 足立 壮一, 多和 昭雄, 伊藤 悦朗, 荒川 浩一, 小川 誠司, 林 泰秀, JPLSG AML委員会.
  • Organizer: 第117回日本小児科学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-04-11 – 2014-04-13
• [Remarks] 国立研究開発法人 国立成育医療研究センターホームページ
  • URL: https://www.ncchd.go.jp