Proteomic explolation of biomarkers for kawasaki disease-associated coronary artery lesions

• Project/Area Number: 26461617
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Kawai Yoko 京都府立医科大学, 医学部附属病院, 専攻医 (60405248)
• Co-Investigator(Kenkyū-buntansha): 池田 和幸 京都府立医科大学, 医学(系)研究科(研究院), 助教 (30507786) ; 中村 明宏 京都府立医科大学, 医学(系)研究科(研究院), 研究員 (50313854) ; 浜岡 建城 京都府立医科大学, 医学(系)研究科(研究院), 教授 (60189602)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 川崎病 / プロテオーム / 血管内皮細胞 / 小胞体ストレス / プロテオミクス / 冠動脈内皮細胞 / 膜型レクチン / iTRAQ / 内皮細胞

Outline of Final Research Achievements

Kawasaki disease is a pdiatric vasculitis. As coronary arteries are preferential targets for the disease, KD frequently involves coronary artery sequela including aneurysms.Thus it is urgent issue to establish reliable and sensitive biomarker for KD-associated coronary artery. In this study, we conducted comparative proteomics of human endothelial cells derived from the coronary arteries and aorta.
As a result, reduced expression was detected in endoplasmic reticulum-associated proteins of the coronary arterial endothelial cells, implying that coronary artery endothelium might be different from that of other vessel in ER-stress sensitivity, glycome of membrane glycoprotens and proteome/glycome of secreted proteins.

Research Products

• [Journal Article] The involvement of the vasa vasorum in the development of vasculitis in animal model of Kawasaki disease. (2014)
  • Author(s): Hamaoka-Okamoto A, Suzuki C, Yahata T, Ikeda K, Nagi-Miura N, Ohno N, Arai Y, Tanaka H, Takamatsu T, Hamaoka K.
  • Journal Title: Pediat Rheumatol Volume: 12 Issue: 1 Pages: 1-12
  • DOI: 10.1186/1546-0096-12-12
  • Peer Reviewed / Open Access
• [Presentation] Possible Implication of Oxidative Stress in Development of Coronary Vascular Lesions in Kawasaki Disease (2014)
  • Author(s): Kenji Hamaoka, Tomoyo Yahata, Chinatsu Suzuki, Akiko Okamoto-Hamaoka, Ayako Yoshioka, Akihiro Nakamura, and Kazuyuki Ikeda
  • Organizer: American Heart Association Scientific Sessions 2014
  • Place of Presentation: Chicago
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] Analysis of the Mechanisms of Intravenous Immunoglobulin-Resistant Kawasaki Disease Using iPS Cell Technology (2014)
  • Author(s): Kazuyuki Iked, Tomonaga Ameku, Yui Nomiya, Masahiro Nakamura, Satoshi Matsui, Tomoyo Yahata, Akiko Okamoto-Hamaoka, Chinatsu Suzuki, Yuki Kuchitsu, Akira Watanabe, Kenji Osafune, Kenji Hamaoka
  • Organizer: American Heart Association Scientific Sessions 2014
  • Place of Presentation: Chicago
  • Year and Date: 2014-11-15 – 2014-11-19