Development of a novel therapy for perinatal brain injury with dedifferentiated fat cells
Project/Area Number |
26461630
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Embryonic/Neonatal medicine
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Research Institution | Nagoya University |
Principal Investigator |
Sato Yoshiaki 名古屋大学, 医学部附属病院, 講師 (30435862)
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Co-Investigator(Renkei-kenkyūsha) |
MATSUMOTO TARO 日本大学, 医学部, 教授 (50366580)
TSUHI MASAHIRO 独立行政法人国立循環器病研究センター, 再生医療部, 室長 (80579467)
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Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 低酸素性虚血性脳症 / 幹細胞 / 新生児低酸素性虚血性脳症 / 脱分化脂肪細胞 / 周産期低酸素性虚血性脳症 |
Outline of Final Research Achievements |
In the present study, we aimed to determine whether the outcome of HIE can be improved by DFAT cell treatment. 24 h after HI insult, DFAT cells were injected at 10^5 cells/pup into the right external jugular vein. The number of cells that stained positive for active caspase-3, ED-1, and 4-HNE decreased in the DFAT-treated group. The HI insult led to a motor deficit according to the rotarod treadmill and cylinder test, where it significantly affected the vehicle group, whereas no difference was confirmed between the DFAT and sham groups. According to in vitro experiments, the cell death rates in the DFAT-CM-treated cells were significantly lower than those in the controls. In the DFAT-CM, several neurotrophic factors, IGF-1、NGF、NT3 were detected, and these factors were increased in the ipsilateral brain after injection of DFAT. Our results indicate that intravenous injection with DFAT cells is effective for ameliorating HI brain injury, possibly via paracrine effects.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Dedifferentiated fat cells, as a novel source for cell therapy to target neonatal hypoxic ischemic encephalopathy.2017
Author(s)
Mikrogeorgiou A, Kondo T, Hattori T, Sugiyama Y, Nakanishi K, Tsuji M, Kazama T, Kano K, Matsumoto T, Hayakawa M, Sato Y
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Journal Title
Developmental Neuroscience
Volume: 印刷中
Issue: 1-4
Pages: 273-286
DOI
Related Report
Peer Reviewed
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[Journal Article] Multicenter observational study comparing sedation/analgesia protocols for laser photocoagulation treatment of retinopathy of prematurity.2015
Author(s)
Sato Y, Oshiro M, Takemoto K, Hosono H, Saito A, Kondo T, Aizu K, Matsusawa M, Futamura Y, Asami T, Terasaki H, Hayakawa M.
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Journal Title
J Perinatol.
Volume: 35
Issue: 11
Pages: 965-9
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Maternal molecular hydrogen administration on lipopolysaccharide-induced mouse fetal brain injury2015
Author(s)
Nakano T, Kotani T, Mano Y, Tsuda H, Imai K, Ushida T, Li H, Miki R, Sumigama S, Sato Y, Iwase A, Hirakawa A, Asai M, Toyokuni S, Kikkawa F.
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Journal Title
Journal of Clinical Biochemistry and Nutrition
Volume: 57
Issue: 3
Pages: 178-182
DOI
NAID
ISSN
0912-0009, 1880-5086
Related Report
Peer Reviewed / Open Access
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[Journal Article] Maternal molecular hydrogen treatment attenuates lipopolysaccharide-induced rat fetal lung injury.2015
Author(s)
Hattori Y, Kotani T, Tsuda H, Mano Y, Tu L, Li H, Hirako S, Ushida T, Imai K, Nakano T, Sato Y, Miki R, Sumigama S, Iwase A, Toyokuni S, Kikkawa F.
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Journal Title
Free radical research.
Volume: 49
Issue: 8
Pages: 1026-37
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Administration of umbilical cord blood cells transiently decreased hypoxic-ischemic brain injury in neonatal rats2015
Author(s)
Hattori, T. Sato, Y. Kondo, T. Ichinohashi, Y. Sugiyama, Y. Yamamoto, M. Kotani, T. Hirata, H. Hirakawa, A. Suzuki, S. Tsuji, M. Ikeda, T. Nakanishi, K. Kojima, S. Blomgren, K. Hayakawa, M.
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Journal Title
Dev Neurosci
Volume: 37
Issue: 2
Pages: 95-104
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Effects of intravenous administration of umbilical cord blood CD34+ cells in a mouse model of neonatal stroke.2014
Author(s)
Tsuji M, Taguchi A, Ohshima M, Kasahara Y, Sato Y, Tsuda H, Otani K, Yamahara K, Ihara M, Harada-Shiba M, Ikeda T, Matsuyama T.
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Journal Title
Neuroscience
Volume: 263
Pages: 148-158
DOI
Related Report
Peer Reviewed
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[Presentation] Intravenous injection with dedifferentiated fat cells ameliorated neonatal hypoxic ischemic brain injury, possibly via paracrine effects2017
Author(s)
Yoshiaki Sato, Alkisti Mikrogeorgiou, Taiki Kondo, Tetsuo Hattori, Yuichiro Sugiyama, Keiko Nakanishi, Masahiro Tsuji, Tomohiko Kazama, Koichiro Kano, Taro Matsumoto, Masahiro Hayakawa
Organizer
International Society for Stem Cell Research Annual meeting
Place of Presentation
ボストン(米国)
Year and Date
2017-06-14
Related Report
Int'l Joint Research
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[Presentation] Dedifferentiated fat cells, as a novel source for cell therapy to target neonatal hypoxic ischemic encephalopathy2017
Author(s)
Alkisti Mikrogeorgiou, Yoshiaki Sato, Taiki Kondo, Tetsuo Hattori, Yuichiro Sugiyama, Keiko Nakanishi, Masahiro Tsuji, Tomohiko Kazama, Koichiro Kano, Taro Matsumoto, Masahiro Hayakawa
Organizer
Pediatric Academic Societies Meeting
Place of Presentation
サンフランシスコ(米国)
Year and Date
2017-05-06
Related Report
Int'l Joint Research
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[Presentation] 新生児低酸素性虚血性脳症に対する自己臍帯血幹細胞治療の臨床試験2015
Author(s)
佐藤義朗, 辻雅弘, 鍋谷まこと, 市場博幸, 岡 明, 田村正徳, 濱崎考史, 北島博之, 船戸正久, 田口明彦, 楠田聡, 森臨太郎, 早川昌弘, 渡部晋一, 新宅治夫
Organizer
第14回日本再生医療学会総会
Place of Presentation
パシフィコ横浜(神奈川県横浜市)
Year and Date
2015-03-19 – 2015-03-21
Related Report
-
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[Presentation] 新生児低酸素性虚血性脳症モデルラットにおける骨髄単核球静脈内投与の治療効果2014
Author(s)
近藤大貴, 佐藤義朗, 杉山裕一朗, 鈴木俊彦, 北瀬悠磨, 片岡英里奈, 伊藤美春, 齊藤明子, 孫田みゆき, 村松友佳子, 辻雅弘, 中西圭子, 早川昌弘
Organizer
第59回日本未熟児新生児学会
Place of Presentation
愛媛県民文化会館(愛媛県松山市)
Year and Date
2014-11-10 – 2014-11-12
Related Report
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[Presentation] 早産児と正期産児におけるProton MR spectroscopyの比較検討2014
Author(s)
兵藤玲奈, 佐藤義朗, 伊藤美春, 杉山裕一朗, 鈴木俊彦, 北瀬悠磨, 齊藤明子, 近藤大貴, 孫田みゆき, 村松友佳子, 城所博之, 早川昌弘
Organizer
第59回日本未熟児新生児学会
Place of Presentation
愛媛県民文化会館(愛媛県松山市)
Year and Date
2014-11-10 – 2014-11-12
Related Report
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[Presentation] HIEモデルマウスにおける抗IL-6 レセプター抗体の効果2014
Author(s)
齊藤明子, 佐藤義朗, 鈴木俊彦, 杉山裕一朗, 伊藤美春, 孫田みゆき, 近藤大貴, 藤巻英彦, 中村裕子, 早川昌弘
Organizer
第50回日本周産期・新生児医学会学術集会
Place of Presentation
シェラトン・グランデ・トーキョーベイ・ホテル(千葉県浦安市)
Year and Date
2014-07-13 – 2014-07-15
Related Report
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[Presentation] Administration of umbilical cord blood cells decreases hypoxic-ischemic brain injury in neonatal rats.2014
Author(s)
Sato Y, Hattori T, Kondo T, Ichinohashi Y, Sugiyama Y, Tsuji M, Ikeda T, Nakanishi K, Blomgren K, Hayakawa M
Organizer
9th Hershey Conference on Developmental Brain Injury
Place of Presentation
Saint Michael, USA
Year and Date
2014-06-02 – 2014-06-08
Related Report
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[Presentation] 周産期脳障害に対する幹細胞療法2014
Author(s)
佐藤義朗
Organizer
第117回日本小児科学会学術集会
Place of Presentation
名古屋国際会議場(愛知県名古屋市)
Year and Date
2014-04-11 – 2014-04-13
Related Report
Invited