| Project/Area Number |
26461643
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大塚 宜一 順天堂大学, 医学部, 客員准教授 (90338335)
東海林 宏道 順天堂大学, 医学(系)研究科(研究院), 准教授 (30365621)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | プロバイオティクス / 消化管粘膜 / 免疫応答 / 低出生体重児 / 母乳 / 腸内細菌 |
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| Outline of Final Research Achievements |
To evaluate effects of Lactobacillus reuteri, Lactobacillus brevis and Bifidobacterium breve on the tight junction function of Caco-2 monolayer culture we established the measurement of TEER (transeptithelial electrical resistance) using volt-ohm meter. Newborn BALB/c mice were divided into dam-rearing pups and pups reared with milk substitute from 3-day-old using a hand-feeding system. In milk substitute-fed group, immune cells, such as the innate immune cells and CD8+T cells, were systemically more activated compared to those in dam-rearing group. We observed a colonization of Bifidobacterium. breve (M-16V) in low birth weight infants by administration of M-16V from soon after their birth detecting M16-V from stools of those infants ages at 1 month and 1 year. This finding shows the importance of early administration of probiotics in low birth weight infants.
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