Elucidation of the regulation by secreted protein MFG-E8, and the development of new therapy

• Project/Area Number: 26461654
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Gunma University
• Principal Investigator: Motegi Sei-ichiro 群馬大学, 大学院医学系研究科, 講師 (20420185)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 創傷治癒 / 悪性黒色腫 / 強皮症 / 線維化 / 間葉系幹細胞

Outline of Final Research Achievements

We studied the role of secreted protein MFG-E8 in wound healing, tumor angiogenesis, fibrosis. Based on the results of this study, we found that MFG-E8 enhances angiogenesis, regulates macrophages, resulting in the acceleration of wound healing, promoting tumor growth, and inhibition of fibrosis. If we can further elucidate the mechanism by which MFG-E8 regulates wound healing, tumor angiogenesis and fibrosis, it will help to understand the pathogenesis of refractory ulcer, malignant tumor, scleroderm, and it can be expected to contribute greatly to the development of new therapy.

Research Products

• [Int'l Joint Research] National Institutes of Health(米国)
• [Journal Article] MFG-E8 drives melanoma growth by stimulating mesenchymal stromal cell-induced angiogenesis and M2 polarization of tumor-associated macrophages (2016)
  • Author(s): Yamada K, Uchiyama A, Uehara A, Perera B, Ogino S, Yokoyama Y, Takeuchi Y, Udey MC, Ishikawa O, and Motegi S
  • Journal Title: Cancer Research Volume: 76 Issue: 14 Pages: 4283-92
  • DOI: 10.1158/0008-5472.can-15-2812
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Protective Effect of MFG-E8 after Cutaneous Ischemia-Reperfusion Injury. (2015)
  • Author(s): Akihiko Uchiyama, Kazuya Yamada, Buddhini Perera, Sachiko Ogino, Yoko Yokoyama, Yuko Takeuchi, Osamu Ishikawa, and Sei-ichiro Motegi
  • Journal Title: Journal of Investigative Dermatology Volume: 135 Issue: 4 Pages: 1157-65
  • DOI: 10.1038/jid.2014.515
  • Peer Reviewed
• [Presentation] Inhibitory regulation of MFGE-8 on fibrosis in systemic sclerosis. (2016)
  • Author(s): Fujiwara C, Uehara A, Yokoyama Y, Ogino S, Uchiyama A, sekiguchi A, Ogino S, Ishikawa O, Motegi S.
  • Organizer: The 41th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Sendai
  • Year and Date: 2016-12-09
  • Int'l Joint Research
• [Presentation] MFG-E8 promotes mesenchymal stem cells-induced angiogenesis (2015)
  • Author(s): Kazuya Yamada, Akihiko Uchiyama, Sachiko Ogino, Buddhini Perera, Yoko Yokoyama, Yuko Takeuchi, Osamu Ishikawa, and Sei-ichiro Motegi
  • Organizer: The 45th Annual ESDR Meeting
  • Place of Presentation: Rotterdam
  • Year and Date: 2015-09-09
  • Int'l Joint Research
• [Presentation] Protective effect of MFG-E8 after cutaneous ischemia-reperfusion injury (2014)
  • Author(s): Akihiko Uchiyama, Kazuya Yamada, Buddhini Perera, Sachiko Ogino, Yoko Yokoyama, Yuko Takeuchi, Osamu Ishikawa, and Sei-ichiro Motegi
  • Organizer: 39th Japanese Society for Investigative Dermatology
  • Place of Presentation: ホテル阪急エキスポパーク（大阪府吹田市）
  • Year and Date: 2014-12-12 – 2014-12-14
• [Presentation] MFG-E8 promotes mesenchymal stem cells-induced angiogenesis in malignant melanoma (2014)
  • Author(s): Kazuya Yamada, Akihiko Uchiyama, Buddhini Perera, Sachiko Ogino, Yoko Yokoyama, Yuko Takeuchi, Osamu Ishikawa, and Sei-ichiro Motegi
  • Organizer: 39th Japanese Society for Investigative Dermatology
  • Place of Presentation: ホテル阪急エキスポパーク（大阪府吹田市）
  • Year and Date: 2014-12-12 – 2014-12-14
• [Remarks] 悪性黒色腫における間葉系幹細胞の役割を解明
  • URL: http://www.gunma-u.ac.jp/information/16246