| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
DNA mismatch repair is an important life system, which is conserved from bacteria to human, and starts by mismatch recognition where seven kinds of enzymes are involved in human. We reported that patients with collagen diseases including inflammatory myopathy carry autoantibodies against four kinds of these enzymes. Further studies clarified that major targets of these antibodies are MLH1 and PMS1 and that some patients who had these antibodies also simultaneously carried other myositis-marker autoantibodies and other patients did not. We have found that commercially available substrates for indirect immunofluorescence tests were not suitable for the screening of autoantibodies against these enzymes and the suitable fixed cultured cells for the screening. Moreover, we have found the possibility that ultraviolet irradiation to cultured keratinocytes might influence the expression of these enzymes (autoantigens).
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