The analysis of regulation of serine protease inhibitors expression in epidermal keratinocytes

• Project/Area Number: 26461658
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Okayama University
• Principal Investigator: Morizane Shin 岡山大学, 大学病院, 助教 (80423333)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: セリンプロテアーゼ / セリンプロテアーゼ阻害因子 / 表皮角化細胞 / SPINK5 / SLPI / エラフィン / アトピー性皮膚炎 / Th2サイトカイン

Outline of Final Research Achievements

High calucium, TNF-alpha, IL-17A, and Toll-like receptor ligands significantly inudced the expression of LEKTI, SLPI, and elafin in normal human epidermal keratinocytes. In addition, high calcium up- and down-regulated trypsin- and chymotrypsin-like serine protease activities in epidermal keratinocytes, respectively.
Immunohistochemical analysis revealed that the expression of LEKTI, SLPI, and elafin were increased in the lesion of psoriasis, rosacea, acne, chronic pyoderma, and herpes zoster.

Research Products

• [Journal Article] Expression of serine protease inhibitors in epidermal keratinocytes is increased by calcium but not 1,25-dihydroxyvitamin D3 or retinoic acid. (2016)
  • Author(s): Kobashi M, Morizane S, Sugimoto S, Sugihara S, Iwatsuki K.
  • Journal Title: British Journal of Dermatology Volume: 印刷中 Issue: 6 Pages: 1525-1532
  • DOI: 10.1111/bjd.15153
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] The expression of serine protease inhibitors are induced by TNF-α and IL-17A in skin inflammatory diseases (2016)
  • Author(s): Satoru Sugihara, Shin Morizane, Saeko Sugimoto, Mina Kobashi, Keiji Iwatsuki
  • Organizer: The 41st Annual Meeting of The Japanese Society for Investigative Dermatology
  • Place of Presentation: 仙台市
  • Year and Date: 2016-12-09
  • Int'l Joint Research
• [Presentation] The expression of serine protease inhibitors in epidermal keratinocytes is increased by calcium, but not 1,25 (OH)2 vitamin D3 or retinoic acid (2016)
  • Author(s): Mina Kobashi, Shin Morizane, Saeko Sugimoto, Satoru Sugihara, Keiji Iwatsuki
  • Organizer: The 41st Annual Meeting of The Japanese Society for Investigative Dermatology
  • Place of Presentation: 仙台市
  • Year and Date: 2016-12-09
  • Int'l Joint Research
• [Presentation] TLR signaling regulates the expression of serine protease inhibitors in epidermal keratinocytes (2016)
  • Author(s): Saeko Sugimoto, Shin Morizane, Satoru Sugihara, Mina Kobashi, Keiji Iwatsuki
  • Organizer: The 41st Annual Meeting of The Japanese Society for Investigative Dermatology
  • Place of Presentation: 仙台市
  • Year and Date: 2016-12-09
  • Int'l Joint Research
• [Presentation] TNF-alpha and IL-17A increase SPINK5 expression in epidermal keratinocytes (2015)
  • Author(s): Satoru Sugihara, Shin Morizane, Saeko Sugimoto, Mina Kobashi, Keiji Iwatsuki
  • Organizer: The 40th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2015-12-11
  • Int'l Joint Research
• [Presentation] TNF-alpha, IL-17A, a Toll-like receptor 3 ligand, and high calcium enhances the expression of SPINK6 in epidermal keratinocytes (2015)
  • Author(s): Saeko Sugimoto, Shin Morizane, Satoru Sugihara, Mina Kobashi, Akira Sunagawa, Keiji Iwatsuki
  • Organizer: The 40th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2015-12-11
  • Int'l Joint Research
• [Presentation] Calcium but not 1,25(OH)2 vitamin D3 or retinoic acid increases SPINK5 expression in epidermal keratinocytes (2015)
  • Author(s): Mina Kobashi, Shin Morizane, Saeko Sugimoto, Satoru Sugihara, Keiji Iwatsuki
  • Organizer: The 40th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2015-12-11
  • Int'l Joint Research
• [Presentation] 慢性炎症性皮膚疾患におけるセリンプロテアーゼ阻害因子の発現とその制御機構 (2014)
  • Author(s): 森実真、岩月啓氏
  • Organizer: 第113回日本皮膚科学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2014-05-30 – 2014-06-01