Dynamics of cellular components of bullae in bullous pemphigoid

• Project/Area Number: 26461664
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Osaka City University
• Principal Investigator: Tsuruta Daisuke 大阪市立大学, 大学院医学研究科, 教授 (90382043)
• Co-Investigator(Kenkyū-buntansha): 小澤 俊幸 大阪市立大学, 大学院医学研究科, 講師 (50570602)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: focal adhesion / BP-IgG / 水疱性類天疱瘡 / ヘミデスモゾーム / フォーカルコンタクト

Outline of Final Research Achievements

We analyzed the compensation of focal adhesion (FA) adhesion after disruption of hemidesmosomes (HD) by bullous pemphigoid (BP-IgG) onto keratinocyte cell culture. The results suggested that expression of FA was inversely up-regulated after down-regulation of HD by BP-IgG, chronologically. The size of FA was increased and the dynamics of FA was decreased after BP-IgG treatment onto keratinocyte by several experiments. This phenomenon maybe explained by compensatory up-regulation of "strength of attachment" by FA from weakend "strength of attachment" by HD. On the other hand, we cannot directly see "strength of attachment" by FAs in itself. We consider that the "strength of attachment" by FA is too weak to be detected by conventional analytical method by us. We have a plan to see it after development of new equipments in the future.

Research Products

• [Journal Article] Characterization of two cases of bullous pemphigoid reactive only with BP230 on Japanese enzyme-linked immunosorbent assays. (2014)
  • Author(s): Sasai S, Nishikawa R, Ohzono A, Hayakawa T, Tsuruta D, Kudoh K, Kikuchi T, Hashiguchi M, Ohata C, Furumura M, Koga H, Ishii N, Hashimoto T
  • Journal Title: Acta Derm Venereol Volume: 94 Issue: 6 Pages: 734-736
  • DOI: 10.2340/00015555-1848
  • Peer Reviewed
• [Book] 今日の診断指針ポケット版 (2015)
  • Author(s): 鶴田大輔
  • Total Pages: 2103
  • Publisher: 医学書院
• [Remarks] 自己免疫性水疱症の病態解明と治療法の開発
  • URL: http://www.med.osaka-cu.ac.jp/Derma/report/reports-01.shtml