New treatment of epidermolysis bullosa by the dermal rearrangement with fibroblasts and mesenchymal stem cells

• Project/Area Number: 26461675
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Hirosaki University
• Principal Investigator: Matsuzaki Yasushi 弘前大学, 医学部附属病院, 講師 (50322946)
• Co-Investigator(Kenkyū-buntansha): 澤村 大輔 弘前大学, 医学研究科, 教授 (60196334)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 表皮水疱症 / 間葉系幹細胞 / 線維芽細胞

Outline of Final Research Achievements

In this study, we analyzed the therapeutic possibilities of the dermal fibroblast and mesenchymal stem cells derived from patients of epidermolysis bullosa. We cultured fibroblasts and mesenchymal stem cells from the skin and fat tissue from COLVII-KO mice and injected the cells intradermally in the dorsum of COLVII-KO mice. We revealed that injected cells produced the rearrangement of the dermal condition and removed the irritated skin changes in the COLVII-KO mice.

Research Products

• [Journal Article] Effcts of halothane anesthesia on electrocardiogram parameters. (2017)
  • Author(s): Nozaki S, Ogata Y, Yonekura M, Han C, Niwa H, Kushikata T, Hirota K, Matsuzaki Y, Tomita H, Imaizumi T, Itagaki S, Sawamura D, Murakami M
  • Journal Title: Hirosaki Med J Volume: 67（2-4） Pages: 129-135
  • Peer Reviewed / Open Access
• [Journal Article] Hyper-IgE syndrome with a novel mutation of the STAT3 gene. (2016)
  • Author(s): Minakawa S, Tanaka H, Kaneko T, Matsuzaki Y, Kono M, Akiyama M, Minegishi Y, Sawamura D.
  • Journal Title: Clin Exp Dermatol Volume: 41 Issue: 6 Pages: 687-689
  • DOI: 10.1111/ced.12865
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Annular leukocytoclastic vasculitis associated with essential mixed cryoglobulinemia. (2016)
  • Author(s): Matsuzaki Y, Jin K, Rokunohe A, Minakawa S, Nakano H, Sawamura D.
  • Journal Title: Eur J Dermatol. Volume: 26（2） Issue: 2 Pages: 186-187
  • DOI: 10.1684/ejd.2015.2704
  • Peer Reviewed
• [Journal Article] A randomized double-blind trial of intravenous (2016)
  • Author(s): Amagai M, Ikeda S, Hashimoto T, Mizuashi M, Fujisawa A, Ihn H, Matsuzaki Y, Ohtsuka M, Fujiwara H, Furuta J, Tago O, Yamagami J, Tanikawa A, Uhara H, Morita A, Nakanishi G, Tani M, Aoyama Y, Makino E, Muto M, Manabe M, et al.
  • Journal Title: J Dermatol Sci Volume: 85（2） Issue: 2 Pages: 77-84
  • DOI: 10.1016/j.jdermsci.2016.11.003
  • Peer Reviewed / Open Access
• [Journal Article] Immunosuppressive effect of adipose-derived stromal cells on imiquimod-induced. (2016)
  • Author(s): Rokunohe A, Matsuzaki Y, Rokunohe D, Sakuraba Y, Fukui T, Nakano H, Sawamura D.
  • Journal Title: J Dermatol Sci Volume: 82 Issue: 1 Pages: 50-53
  • DOI: 10.1016/j.jdermsci.2015.12.007
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Modified sympatheticsympathetic nerve regulation in AKAP5-null mice. (2016)
  • Author(s): Han C, Tomita H, Ohba T, Nishizaki K, Ogata Y, Matsuzaki Y, Sawamura D, Yanagisawa T, Osanai T, Imaizumi T, Matsubara A, Adachi T, Ono K, Okumura K, Murakami M.
  • Journal Title: Biochem Biophys Res Commun. Volume: 469（4） Issue: 4 Pages: 897-902
  • DOI: 10.1016/j.bbrc.2015.12.057
  • Peer Reviewed / Open Access
• [Journal Article] Superficial epidermolytic ichthyosis caused by a novel KRT2 mutation. (2015)
  • Author(s): Akasaka E, Minakawa S, Rokunohe D, Toyomaki Y, Matsuzaki Y, Sawamura D, Nakano H
  • Journal Title: J Dermatol Sci. Volume: 79 Issue: 1 Pages: 182-184
  • DOI: 10.1016/j.jdermsci.2015.04.006
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of the histamine H1 receptor in the regulation of sympathetic nerve activity. (2015)
  • Author(s): Murakami M, Yoshikawa T, Nakamura T, Ohba T, Matsuzaki Y, Sawamura D, Kuwasako K, Yanagisawa T, Ono K, Nakaji S, Yanai K.
  • Journal Title: Biochem Biophys Res Commun. Volume: 458 Issue: 3 Pages: 584-589
  • DOI: 10.1016/j.bbrc.2015.02.009
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Oral lichen planus with antibodies to desmogleins 1 and 3. (2015)
  • Author(s): Kinjyo C, Kaneko T, Korekawa A, Rokunohe A, Aizu T, Matsuzaki Y, Nakano H, Sawamura D.
  • Journal Title: J Dermatol Volume: 42 Issue: 1 Pages: 40-41
  • DOI: 10.1111/1346-8138.12661
  • Peer Reviewed / Open Access
• [Journal Article] Oral lichen planus with antibodies to desmogleins 1 and 3. (2015)
  • Author(s): Kinjyo C, Kaneko T, Korekawa A, Rokunohe A, Aizu T, Matsuzaki Y, Nakano H, Sawamura D.
  • Journal Title: J Dermatol. Volume: 42(1) Pages: 40-1
  • Peer Reviewed / Open Access
• [Journal Article] Functional analysis of LEMD3 mutation in Buschke-Ollendorff syndrome. (2014)
  • Author(s): Chihiro Kinjo, Hajime Nakano, Ayumi Korekawa, Yuka Toyomaki, Yasushi Matsuzaki, Sawamura Daisuke.
  • Journal Title: Hirosaki Med J. Volume: 65 Pages: 21-6
  • NAID: 110009794397
  • Peer Reviewed
• [Journal Article] Pemphigoid gestationis with prepartum flare. (2014)
  • Author(s): Minakawa S, Kaneko T, Rokunohe D, Nakajima K, Matsuzaki Y, Nakano H, Hashimoto T, Sawamura D
  • Journal Title: J Dermatol Volume: 41 Issue: 9 Pages: 580-851
  • DOI: 10.1111/1346-8138.12576
  • Peer Reviewed / Open Access
• [Journal Article] Case of oculocutaneous albinism complicated with squamous cell carcinoma, Bowen's disease and actinic keratosis. (2014)
  • Author(s): Minakawa S, Kaneko T, Matsuzaki Y, Akasaka E, Mizukami H, Abe Y, Hozumi Y,
  • Journal Title: J Dermatol． Volume: 41 Issue: 9 Pages: 863-864
  • DOI: 10.1111/1346-8138.12597
  • Peer Reviewed / Open Access
• [Journal Article] Effects of Propofol on Electrocardiogram Measures in Mice (2014)
  • Author(s): Shintaku T, Ohba T, Niwa H, Kushikata T, Hirota K, Ono K, Matsuzaki Y, Imaizumi T, Kuwasako K, Sawamura D, Murakami M.
  • Journal Title: Journal of Pharmacological Sciences Volume: 126 Issue: 4 Pages: 351-358
  • DOI: 10.1254/jphs.14181FP
  • NAID: 130004704287
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ultraviolet B radiation-stimulated urocortin 1 is involved in tyrosinase-related protein 1 production in human melanoma HMV-II cells. (2014)
  • Author(s): Watanuki Y, Kageyama K, Takayasu S, Matsuzaki Y, Iwasaki Y, Daimon M.
  • Journal Title: Peptides. Volume: 61 Pages: 93-7
  • DOI: 10.1016/j.peptides.2014.09.009
  • Peer Reviewed / Open Access
• [Presentation] Therapeutic and immunomodulatory effects of adipose-derived stromal cells on psoriasis animal model (2016)
  • Author(s): Yasushi Matsuzaki, Akiko Rokunohe, Daiki Rokunohe, Eiko Makita, Hajime Nakano, Daisuke Sawamura
  • Organizer: 第41回日本研究皮膚科学会
  • Place of Presentation: 仙台国際センター（宮城県・仙台市）
  • Year and Date: 2016-12-09
  • Int'l Joint Research
• [Presentation] Functional analysis of LEMD3 mutation in Buschke-Ollendorff Syndrome (2016)
  • Author(s): Hagiwara C, Nakano H, Korekawa A, Toyomaki Y, Matsuzaki Y, Sawamura D
  • Organizer: 13th European Society for Pediatric Dermatology
  • Place of Presentation: Paris (France)
  • Year and Date: 2016-05-26
  • Int'l Joint Research
• [Presentation] Acral pseudolymphomatous angiokeratoma of children ( APACHE ) : a case report with immunohistochemical study (2016)
  • Author(s): Takiyoshi N, Kaneko Y, Matsuzaki Y, Nakano H, Sawamura D
  • Organizer: 22nd Regional Conference of Dermatology
  • Place of Presentation: Singapore (Singapore)
  • Year and Date: 2016-04-21
  • Int'l Joint Research
• [Presentation] アトピー性皮膚炎（AD）と好酸球性胃腸炎患者におけるフィラグリン遺伝子（FLG）変異の検討 (2016)
  • Author(s): 皆川智子、金子高英、松﨑康司、中野 創、澤村大輔、平賀寛人、櫻庭裕丈、河野通浩、秋山真志
  • Organizer: 第79回日本皮膚科学会東京・東部支部合同学術大会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2016-02-20
• [Presentation] Richner-Hanhart症候群（高チロシン血症Ⅱ型）の1例 (2016)
  • Author(s): 赤坂英二郎、滝吉典子、松﨑康司、櫻庭裕佑、是川あゆ美、会津隆幸、豊巻由香、中野 創、澤村大輔
  • Organizer: 日本皮膚科学会岩手地方会第373回例会
  • Place of Presentation: マリオス（岩手県盛岡市）
  • Year and Date: 2016-02-06
• [Presentation] 手指のチアノーゼを呈しdigital subtraction angiography (DSA) により動脈閉塞性疾患を鑑別した全身性強皮症の一例 (2016)
  • Author(s): 神 可代、松﨑康司、中野 創、澤村大輔、櫻庭裕丈、対馬史泰
  • Organizer: 日本皮膚科学会青森地方会第375回例会
  • Place of Presentation: ホテルニューキャッスル（青森県・弘前市）
• [Presentation] 当科通院中の乾癬患者における臨床検査的検討 (2016)
  • Author(s): 皆川智子、金子高英、松﨑康司、中野 創、澤村大輔、山田真博、齋藤紀先、萱場広之
  • Organizer: 日本皮膚科学会青森地方会第376回例会
  • Place of Presentation: ホテル青森（青森県・青森市）
• [Presentation] 当科における乾癬治療の現状とご紹介頂いた患者の治療経過 (2015)
  • Author(s): 松﨑康司
  • Organizer: 第122回弘前皮膚科専門医会
  • Place of Presentation: ホテルニューキャッスル（青森県弘前市）
  • Year and Date: 2015-11-25
• [Presentation] 新規ケラチン2遺伝子変異により生じた表在性表皮融解性魚鱗癬 (2015)
  • Author(s): 赤坂英二郎、中野 創、皆川智子、六戸大樹、豊巻由香、松﨑康司、澤村大輔
  • Organizer: 第30回角化症研究会
  • Place of Presentation: 経団連会館（東京都千代田区）
  • Year and Date: 2015-08-01
• [Presentation] 環状紅斑、紫斑を呈したクリオグロブリン血症性血管炎の1例 (2015)
  • Author(s): 松﨑康司、神 可代、中野 創、澤村大輔、山口公平、中村典雄
  • Organizer: 第38回皮膚脈管・膠原病研究会
  • Place of Presentation: 東京
  • Year and Date: 2015-01-23 – 2015-01-24
• [Presentation] 色素性乾皮症バリアント群の1例 (2014)
  • Author(s): 六戸亜希子、松﨑康司、中野 創、澤村大輔、横山祥平、森脇真一
  • Organizer: 青森地方会第368回例会
  • Place of Presentation: 弘前市
  • Year and Date: 2014-11-16
• [Presentation] CHARACTERIZATION OF STIMULATOR OF INTERFERON GENES (STING) EXPRESSION IN HUMAN EPIDERMAL KERATINOCYTES (2014)
  • Author(s): Yasushi Matsuzaki, Yohei Nishikawa, Akiko Rokunohe, Hajime Nakano, Daisuke Sawamura
  • Organizer: 弘前国際移植シンポジウム2014
  • Place of Presentation: 弘前市
  • Year and Date: 2014-10-31
• [Presentation] 複数の神経ブロックを併用して腋窩郭清を施行した重症型表皮水疱症の1例 (2014)
  • Author(s): 金子高英、会津隆幸、松井彰伸、滝吉典子、六戸大樹、松﨑康司、中野 創、澤村大輔、北山眞任、橋本 浩、廣田和美
  • Organizer: 第29回日本皮膚外科学会
  • Place of Presentation: 和歌山市
  • Year and Date: 2014-09-13 – 2014-09-14