| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Outline of Final Research Achievements |
Herpes simplex virus type 2 (HSV-2) is a sexually transmitted pathogen that is widely prevalent around the world. To date, it is not fully understood whether the time of infection is a variable influencing the outcome of HSV-2 pathology. To address the issues, wild-type mice housed under 12-hour light/12-hour dark conditions (the light was turned on at Zeitgeber time [ZT] 0, and the light was turned off at ZT12) were intradermally infected with HSV-2 either at ZT6 (rest phase) or at ZT18 (active phase). We found that the clinical severity and mortality were higher in mice infected at active phase than at rest phase, depending on circadian expression of HSV-2 receptor Nectin1 in the skin that is likely controlled by the molecular clock. In addition, the degree of the host innate immune response was also less severe in the skin of mice infected at ZT6 than at ZT18. These findings provide new insight into previously unknown aspects of HSV-2 infection and prevention of the disease.
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