The role of plasmacutoid dendritic cells in the pathogenesis of alopecia areata

• Project/Area Number: 26461689
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Ito Taisuke 浜松医科大学, 医学部附属病院, 講師 (90293638)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 形質細胞様樹状細胞 / IFN-alpha / SiglecH / 円形脱毛症 / Chemerin / pDCs / インターフェロンアルファ / MHC class I / CXCL10 / 細胞走化性 / ケモカイン / ケメリン / 免疫寛容

Outline of Final Research Achievements

Alopecia areata (AA) model mouse, C3H/HeJ mouse was employed in this study, Lymphonode cells from C3H/HeJ mouse with AA were activated with IL-2, IL-7 and IL-15 for 5 days. Then, these activated LN cells were intracutaneously injected into healthy C3H/HeJ mouse. The frequency of pDCs were significantly higher in non lesional skin in AA mouse. Furthermore, pDCs from spleen cells induced alopecia lesions in healthy C3H/HeJ mouse. PDCs might be important initiator of AA in C3H/HeJ mouse.

Research Products

• [Journal Article] Similarities of dermoscopic findings in alopecia areata between human and C3H/HeJ mouse. (2016)
  • Author(s): Suzuki T, Tokura Y, Ito T
  • Journal Title: J Dermatol Sci Volume: 83 Issue: 2 Pages: 154-157
  • DOI: 10.1016/j.jdermsci.2016.04.007
  • Peer Reviewed
• [Journal Article] h1 Chemokine CXCL10 and Alopecia Areata: The Possible Target for the Treatment of Alopecia Areata. (2016)
  • Author(s): Ito T, Fujiyama T, Tokura Y
  • Journal Title: J Clin Cell Immunol Volume: 7 Issue: 5
  • DOI: 10.4172/2155-9899.1000453
  • Peer Reviewed / Open Access
• [Journal Article] Distinctive downmodulation of plasmacytoid dendritic cell functions by vitamin D3 analogue calcipotriol. (2016)
  • Author(s): Suzuki T, Tatsuno K, Ito T, Sakabe J, Funakoshi A, Tokura Y:
  • Journal Title: J Dermatol Sci Volume: 84 Issue: 1 Pages: 71-79
  • DOI: 10.1016/j.jdermsci.2016.06.003
  • Peer Reviewed
• [Journal Article] Similarities of dermoscopic findings in alopecia areata between human and C3H/HeJ mouse (2016)
  • Author(s): Suzuki T, Tokura Y, Ito T
  • Journal Title: J Dermal Sci Volume: in press
  • Peer Reviewed
• [Journal Article] Topical application of vitamin D3 analogue and corticosteroid to psoriasis plaques synergistically depresses skin infiltration of Th17 cells and their ex vivo expansion (2016)
  • Author(s): Fujiyama T, Ito T, Umayahara T, Ikeya S, Tatsuno K, Funakoshi A, Hashizume H, Tokura Y.
  • Journal Title: J Allergy Clin Immunol Volume: in press
  • Peer Reviewed
• [Journal Article] Emergence of photosensitivity with decreased Treg cells in mycosis fungoides patient treated with anti-CCR4 antibody mogamulizumab. (2016)
  • Author(s): Tatsuno K, Sano T, Fukuchi K, Kuriyama S, Aoshima M, Kasuya A, Fujiyama T, Ito T, Tokura Y
  • Journal Title: Acta Derm Venereol. Volume: 96 Issue: 3 Pages: 420-421
  • DOI: 10.2340/00015555-2257
  • Peer Reviewed
• [Journal Article] RS3PE syndrome with oedematous skin histopathologically characterized by granulomatous change. (2016)
  • Author(s): Nakazawa S, Mori T, Ito T, Tokura Y
  • Journal Title: Br J Dermal Volume: in press Issue: 6 Pages: 1420-1422
  • DOI: 10.1111/bjd.14513
  • Peer Reviewed
• [Journal Article] Antihistaminic drug olopatadine downmodulates T cell chemotaxis toward CCL17 in patients with atopic dermatitis (2015)
  • Author(s): Ito T, Tatsuno K, Fujiyama T, Sakabe J, Tokura Y.
  • Journal Title: Allergology International Volume: 64 Issue: 2 Pages: 200-202
  • DOI: 10.1016/j.alit.2014.10.008
  • NAID: 130005290340
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 新しい円形脱毛症の病態理解とそれに基づく治療展開 (2015)
  • Author(s): 伊藤泰介
  • Journal Title: 皮膚と美容 Volume: 47 Pages: 11-14
• [Journal Article] The role of cytokines and chemokines in the T-cell-mediated autoimmune process in alopecia areata. (2014)
  • Author(s): Ito T, Tokura Y.
  • Journal Title: Exp Dermatol Volume: 23 Issue: 11 Pages: 787-791
  • DOI: 10.1111/exd.12489
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Exacerbation of alopecia areata during pegylated interferon alpha-2b and ribavirin therapy, possibly due to the collapse of hair follicle immune privilege. (2014)
  • Author(s): Ito T, Suzuki T, Funakoshi A, Tokura Y.
  • Journal Title: Eur J Dermatol Volume: 24 Issue: 5 Pages: 631-633
  • DOI: 10.1684/ejd.2014.2421
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 円形脱毛症と男性型脱毛症の治療総論 (2014)
  • Author(s): 伊藤泰介
  • Journal Title: Derma Volume: 225 Pages: 1-8
• [Journal Article] 脱毛症治療 スタンダードと職人芸 新しい脱毛症病態の考え方とそれを活かした診療 (2014)
  • Author(s): 伊藤泰介
  • Journal Title: 日本皮膚科学会雑誌 Volume: 124 Pages: 2870-2873
• [Journal Article] セマフォリン3Aトランスジェニックマウスにおける毛組織や皮膚の免疫環境でのセマフォリン3Aの役割の解明と皮膚炎症に対する新規治療軸の開発 (2014)
  • Author(s): 伊藤泰介
  • Journal Title: コスメトロジー研究報告 Volume: 22 Pages: 68-71
  • NAID: 40020195080
• [Presentation] Chemokine receptor CCR5 is the novel target for the treatment of alopecia areata. (2016)
  • Author(s): Ito T, Suzuki T, Fujiyama T, Tokura Y
  • Organizer: 46th Annual ESDR Meeting
  • Place of Presentation: Munich
  • Year and Date: 2016-09-07
  • Int'l Joint Research
• [Presentation] Similarities of dermoscopic and immunological findings in alopecia areata between human and C3H/HeJ mouse. (2016)
  • Author(s): Ito T, Suzuki T, Tokura Y
  • Organizer: Asia-Pacific Combined Dermatology Research Conference 2016.
  • Place of Presentation: Noosa
  • Year and Date: 2016-08-25
  • Int'l Joint Research
• [Presentation] Chemokine receptor CCR5 is the novel target for the treatment of alopecia areata. (2015)
  • Author(s): Ito T, Suzuki T, Sakabe JI, Fujiyama T, Funakoshi A, Tokura Y
  • Organizer: 9th World Congress of Hair Research.
  • Place of Presentation: Miami
  • Year and Date: 2015-11-18
  • Int'l Joint Research
• [Presentation] Plasmacytoid dendritic cells as a possible key player in the initiation phase of alopecia areata in the C3H/HeJ mouse. (2015)
  • Author(s): Ito T, Suzuki T, Sakabe JI, Fujiyama T, Funakoshi A, Tokura Y
  • Organizer: 45th Annual ESDR Meeting. European Society for Investigative Dermatology.
  • Place of Presentation: Rotterdam, the Netherlands
  • Year and Date: 2015-09-09
  • Int'l Joint Research
• [Presentation] lasmacytoid dendritic cells as a key player in the induction of alopecia areata. (2014)
  • Author(s): Ito T, Suzuki T, Funakoshi A, Tokura Y
  • Organizer: The 39th Annual Meeting of the Japanese Society for lnvestigative Dermatology
  • Place of Presentation: Osaka
  • Year and Date: 2014-12-12 – 2014-12-13
• [Presentation] 円形脱毛症の治療 (2014)
  • Author(s): 伊藤泰介
  • Organizer: 第19回日本臨床毛髪学会
  • Place of Presentation: 岡山
  • Year and Date: 2014-11-23
  • Invited
• [Presentation] 免疫からみた脱毛症の病態 (2014)
  • Author(s): 伊藤泰介
  • Organizer: 第44回日本皮膚アレルギー・接触皮膚炎学会総会学術大会
  • Place of Presentation: 仙台
  • Year and Date: 2014-11-21 – 2014-11-23
  • Invited
• [Presentation] 苦手意識をのりこえる脱毛症診療 (2014)
  • Author(s): 伊藤泰介
  • Organizer: 静岡県皮膚科医会
  • Place of Presentation: 静岡
  • Year and Date: 2014-11-15
  • Invited
• [Presentation] Plasmacytoid dendritic cells as a possible key player in the pathogenesis of alopecia areata (2014)
  • Author(s): Ito T, Suzuki T, Sakabe JI, Funakoshi A, Tokura Y
  • Organizer: 44th Annual Meeting of the European Society for Dermatological Research
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2014-09-10
• [Presentation] 新しい脱毛症病態の考え方とそれを活かした診療 (2014)
  • Author(s): 伊藤泰介
  • Organizer: 第113回日本皮膚科学会総会
  • Place of Presentation: 京都
  • Year and Date: 2014-05-30 – 2014-06-02
  • Invited
• [Presentation] Autoimmune hair loss alopecia areata and chemokines/cytokines for mediator of inflanmmmation. (2014)
  • Author(s): Ito T
  • Organizer: 8th World Congress for Hair Research
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2014-05-16
  • Invited
• [Presentation] 内科医が遭遇しうる脱毛症とその診断ポイント (2014)
  • Author(s): 伊藤泰介
  • Organizer: 第111回日本内科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2014-04-11 – 2014-04-12
  • Invited