Development of prevention method using liver sinusoidal endothelial cells for food allergy

• Project/Area Number: 26461693
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Hiroshima University
• Principal Investigator: Tanaka Akio 広島大学, 医歯薬保健学研究院(医), 助教 (70714088)
• Co-Investigator(Kenkyū-buntansha): 柳瀬 雄輝 広島大学, 医歯薬保健学研究院(医), 助教 (40452586)
• Co-Investigator(Renkei-kenkyūsha): HIDE MICHIHIRO 広島大学, 大学院医歯薬保健学研究院, 教授 (50284188) ; OHDAN HIDEKI 広島大学, 大学院医歯薬保健学研究院, 教授 (10363061)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 免疫寛容 / 経口免疫寛容

Outline of Final Research Achievements

We hypothesized that liver sinusoidal endothelial cells (LSECs) induces immune tolerance to food antigens, and investigated whether LSECs endocytosed ova albumin (OVA) are able to induce immune tolerance to OVA by using mouse model. However, we found that some mice after transplantation of OVA-endocytosed-LSECs failed to induce immune tolerance and showed increase of serum OVA specific IgE. Although LSECs is described as one of antigen presenting cells that tolerize CD8 T cells, it is not entirely fitted with humoral immunity in our experimental settings. Our findings suggest that LSECs can contribute to exacerbation of food allergy on certain conditions.