Study of gene amplification and metastasis in melanoma
| Project/Area Number |
26461700
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Saitama Medical University (2016-2017)
Takasaki University of Health and Welfare (2014-2015) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
斎藤 克代 高崎健康福祉大学, 薬学部, 助手 (90455288)
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| Co-Investigator(Renkei-kenkyūsha) |
SATO Atsuko 自治医科大学, 医学部, 助教 (50382916)
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| Research Collaborator |
HORIUCHI Yutaka 埼玉医科大学, 医学部, 講師 (30608906)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | がん / メラノーマ / 遺伝子機能 / 上皮間葉転換 / 細胞運動 / シグナル伝達 / 浸潤 / 転移 / 遺伝子増幅 |
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| Outline of Final Research Achievements |
We developed a luciferase-based luminescent assay system to evaluate cell migration using transformed NIH3T3 cells, and the assay system allowed to screen chemicals to inhibit cell migration. Subsequently, the mevalonate pathway appeared critical for migration in cancerous cells, including melanoma. Furthermore, we examined an amplified gene expression pattern using human melanoma cell lines that xenogeneically metastasized to the brain of NOD/SCID mice. Data suggested that 99 candidate genes were highly expressed in progressed melanoma. Further in silico analysis also suggested that some of candidate genes are associated in the biology of melanoma. Perhaps, the function of these candidate genes may be implicated in melanoma-specific tumor progression and its therapeutic resistance.
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Report
(5 results)
Research Products
(41 results)
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[Journal Article] In vivo imaging xenograft models for the evaluation of anti-brain tumor efficacy of targeted drugs2017
Author(s)
Kita K, Arai S, Nishiyama A, Taniguchi H, Fukuda K, Wang R, Yamada T, Takeuchi S, Tange S, Tajima A, Nakada M, Yasumoto K, Motoo Y, Murakami T, Yano S.
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Journal Title
Cancer Med.
Volume: 6
Pages: 2972-2983
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Establishment of Highly Metastatic KRAS Mutant Lung Cancer Cell Sublines in Long-term Three-dimensional Low Attachment Cultures.2017
Author(s)
Nakano T, Kanai Y, Amano Y, Yoshimoto T, Matsubara D, Shibano T, Tamura T, Oguni S, Katashiba S, Ito T, Murakami Y, Fukayama M, Murakami T, Endo S, Niki T.
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] ZEB1-regulated inflammatory phenotype in breast cancer cells.2017
Author(s)
Katsura A, Tamura Y, Hokari S, Harada M, Morikawa M, Sakurai T, Takahashi K, Mizutani A, Nishida J, Yokoyama Y, Morishita Y, Murakami T, Ehata S, Miyazono K, Koinuma D
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Journal Title
Mol Oncol.
Volume: 11
Pages: 1241-1262
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Maltotriose Conjugation to a Chlorin Derivative Enhances the Antitumor Effects of Photodynamic Therapy in Peritoneal Dissemination of Pancreatic Cancer.2017
Author(s)
Kato A, Kataoka H, Yano S, Hayashi K, Hayashi N, Tanaka M, Naitoh I, Ban T, Miyabe K, Kondo H, Yoshida M, Fujita Y, Hori Y, Natsume M, Murakami T, Narumi A, Nomoto A, Naiki-Ito A, Takahashi S, Joh T
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Journal Title
Mol Cancer Ther.
Volume: 印刷中
Pages: 1124-1132
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] An in vivo screening system to identify tumorigenic genes.2017
Author(s)
Ihara T, Hosokawa Y, Kumazawa K, Ishikawa K, Fujimoto J, Yamamoto M, Murakami T, Goshima N, Ito E, Watanabes S, Semba K.
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Journal Title
Oncogene
Volume: 36
Pages: 2023-2029
DOI
Related Report
Peer Reviewed
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[Journal Article] Gene therapy with SOCS1 for gastric cancer induces G2/M arrest and has an anti-tumor effect on peritoneal carcinomatosis2015
Author(s)
Natatsuka R, Takahashi T, Serada S, Fujimoto M, Ookawara T, Nishida T, Hara H, Nishigaki T, Harada E, Murakami T, Miyazaki Y, Makino T, Kurokawa Y, Yamasaki M, Miyata H, Nakajima K, Takiguchi S, Kishimoto T, Mori M, Doki Y, Naka T.
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Journal Title
Br J Cancer.
Volume: 113(3)
Pages: 433-42
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] In Vitro and In Vivo Characterization of KHK2455, a Highly Potent and Selective Indoleamine 2,3-dioxygenase 1 (IDO1) Inhibitor with a Novel Mechanism of Action.2017
Author(s)
Mie M, Kunieda K, Koshiba S, Murakami T, Horita S, Fukuda Y, Ishii T, Nakai R, Nakamura K.
Organizer
Society for Immunotherapy of Cancer
Related Report
Int'l Joint Research
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