Melanocyte and iPS cell research for treatment of vitiligo and melanoma
Project/Area Number |
26461704
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Dermatology
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Research Institution | St. Marianna University School of Medicine |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | メラノサイト / 皮膚色素細胞 / iPS細胞 / 白斑 / 悪性黒色腫 / 白髪 / ヌードマウス / ヒトiPS細胞 / メラノブラスト / 活性型ビタミンD3 / レチノイド |
Outline of Final Research Achievements |
We established a new in vitro approach to generate induced pluripotent stem (iPS) cell-derived human melanocytes which have higher proliferation rates and increased melanin production compared to melanocytes prepared by previously reported approaches. We designed two original methods, transferring black colonies by pipet and recovering black cell pellets from centrifuged medium, and numerous human iPS cell-derived melanocytes proliferated in gelatinous dishes coated with Matrigel after 12 days. We also succeeded in inducing melanin pigmentation in nude mouse skin in vivo using those human iPS cell-derived melanocytes. We propose that this new method using iPS cells established from T cells in the blood of normal human volunteers could be applied clinically to develop regenerative medicine and cell therapies for various forms of human pigmentation disorders.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Transcription factor LSF (TFCP2) inhibits melanoma growth.2016
Author(s)
Goto Y, Yajima I, Kumasaka M, Ohgami N, Tanaka A, Tsuzuki T, Inoue Y, Fukushima S, Ihn H, Kyoya M, Ohashi H, Kawakami T, Bennett DC, Kato M.
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Journal Title
Oncotarget.
Volume: 19
Issue: 3
Pages: 2739-2790
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A case of hemorrhage due to cutaneous metastatic malignant melanoma controlled with transcatheter arterial embolization and Mohs' paste2015
Author(s)
大橋洋之, 松岡摩耶, 大石佳奈, 岡野達郎, 武藤真悠子, 久保悠, 齋藤千尋, 門野岳史, 川上民裕, 相馬良直, 生野雅也
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Journal Title
Skin Cancer
Volume: 30
Issue: 2
Pages: 108-112
DOI
NAID
ISSN
0915-3535, 1884-3549
Related Report
Peer Reviewed
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