Studies on intracellular parasitization of Mycobacterium leprae for the development of laboratory examination of leprosy
| Project/Area Number |
26461708
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Ishii Norihisa 国立感染症研究所, ハンセン病研究センター, センター長 (50159670)
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| Co-Investigator(Kenkyū-buntansha) |
鈴木 幸一 帝京大学, 医療技術学部, 教授 (20206478)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ハンセン病 / らい菌 / 細胞内寄生 / 脂質代謝 / 脂質 / 皮膚抗酸菌症 |
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| Outline of Final Research Achievements |
We performed DNA microarray analysis of Mycobacterium lepra (M. leprae)-infected cultured macrophage cells. We found that mRNA expression of transcription factors, peroxisome proliferator-activated receptors (PPAR-γ and PPAR-δ) and their downstream genes related to lipid metabolism were significantly induced following M. leprae infection. We then purified mRNA from skin smear samples taken from multibacillary patients and analyzed mRNA expressions of those genes essential for lipid accumulation by RT-PCR. The expression of those genes was significantly induced in the patients, but returned to normal level after multi-drug therapy. These results suggest that RT-PCR analysis of these genes would be an useful tool for the laboratory examination to evaluate disease states of leprosy patients and also to evaluate efficacy of treatment.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Integrated control and management of neglected tropical skin diseases2017
Author(s)
Mitja O, Marks M, Bertran L, Kollie K, Argaw D, Fahal AH, Fitzpatrick C, Fuller LC, Garcia Izquierdo B, Hay R, Ishii N, Johnson C, Lazarus JV, Meka A, Murdoch M, Ohene SA, Small P, Steer A, Tabah EN, Tiendrebeogo A, Waller L, Yotsu R, Walker SL, Asiedu K
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Journal Title
PLos Negl Trop Dis
Volume: 11
Issue: 1
Pages: e0005136-e0005136
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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