| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We analyzed pathological tau in cases with Alzheimer's disease, Pick's disease, corticobasal degeneration and progressive supranuclear palsy. We found that immunoblot analysis of trypsin-resistant tau showed the different band patterns of the fragments among these diseases, reflecting different conformations of tau molecular species. Protein sequence and mass spectrometric analyses revealed the carboxyl-terminal region of tau comprises the protease-resistant core units of the tau aggregates. These unique assembled tau cores may be used to classify and diagnose disease strains. Furthermore, to investigate the influence of a decline in progranulin on neurodegenerative diseases, brains of granulin mutation cases were analyzed. Results showed a neuronal and glial accumulation of tau andα-synuclein in addition to that of TDP-43. These results suggest that progranulin reduction might be the cause of multiple proteinopathies due to the accelerating accumulation of abnormal proteins.
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