Construction of strategy to develop new brain tumor imaging agents targeting cancer stem cells
| Project/Area Number |
26461801
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Ibaraki Prefectural University of Health Science |
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Principal Investigator |
Shimamoto Naoto 茨城県立医療大学, 保健医療学部, 准教授 (80295435)
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| Co-Investigator(Kenkyū-buntansha) |
大西 健 茨城県立医療大学, 保健医療学部, 教授 (50152195)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | がん幹細胞 / 脳腫瘍 / 分子イメージング / CD133 / 抗体イメージング / リポソーム / アミノ酸トランスポーター / 薬物動態制御 / 放射性人工アミノ酸 / 膜蛋白 / 機能イメージング / フローサイトメトリー / グリオーマ細胞株 / 核医学 |
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| Outline of Final Research Achievements |
It is said that there are cells capable of reconstructing it in the cancer tissue (cancer stem cells), and a cancer tissue as a hierarchical cell group is constructed. In order to develop a reagent for cancer stem cell imaging, fluorescent dye-encapsulated liposome coated with marker of brain tumor and colon cancer stem cell CD133AC133 antibody was prepared and evaluated by CD133 overexpression human glioblastoma cell T98G cell. The prepared liposomes had distribution on the cell surface but could not be taken into the inside of the cell due to low activity of CD133 internalization. Cancer stem cells are said to be difficult to image, but the possibility of antibody imaging was suggested.
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Report
(4 results)
Research Products
(11 results)
