Hybrid MRI contrast agent and basic comparison of performance with other contrast agents using animal experiment

Research Project

Project/Area Number	26461807
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Radiation science
Research Institution	Komazawa University
Principal Investigator	Shimada Morio 駒澤大学, 医療健康科学部, 教授 (20196489)
Co-Investigator(Kenkyū-buntansha)	吉川宏起駒澤大学, 医療健康科学部, 教授 (10272494) 岡田朋子駒澤大学, 医療健康科学部, 講師 (60409795) 森口央基駒澤大学, 医療健康科学部, 教授 (70296705) 桐生茂東京大学, 医科学研究所, 准教授 (20313124)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000) Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywords	MRI / 造影剤 / 蛍光イメージング / コラーゲンペプチド / Gd / マウス / Gd-DOTA / ペプチド / 集積能 / ICG / NASH / 蛍光 / NH2-DTPA / USPIO / SPIO / 間質性MR Lymphography / MR造影剤 / 動物実験
Outline of Final Research Achievements	Evaluation of lymph flow in interstitium was possible with iron preparation. Depending on the selection of the particle diameter and dilution concentration, there are cases in which contrast imaging is not available, and appropriate selection is necessary.We prepared a mouse of NASH model as a material to evaluate liver function and compared it with normal mouse.We administered ICG to both groups of mice. We imaged by IVIS every 5 minutes, And analyzed these images.As a result of examination, the progression of NASH could be detected from the fluorescence by ICG.A novel conjugate, PHG10 dye, was synthesized using a collagen peptide and a near-infrared (NIR)-responsive dye to achieve targeted cytotoxicity.Using biopolymer PPG as a starting material, Gd-PPG was obtained through multi-step synthesis process.