| Project/Area Number |
26461847
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Kanazawa University |
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Principal Investigator |
Taki Junichi 金沢大学, 附属病院, 講師 (10251927)
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| Co-Investigator(Kenkyū-buntansha) |
柴 和弘 金沢大学, 学際科学実験センター, 教授 (40143929)
小川 数馬 金沢大学, 新学術創成研究機構, 准教授 (30347471)
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| Research Collaborator |
WAKABASHI Hiroshi 金沢大学, 附属病院, 助教 (60622818)
INAKI Anri 金沢大学, 医学系, 協力研究員 (40645131)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | myocardial ischemia / remodeling / tenascin C / angiogenesis / RGD / apoptosis / annexin V / ventricular remodeling / myocardial infarction / Tenascin-C / methionine / macrophage / インテグリン / 血管新生 / 左室リモデリング / 心筋梗塞 / 虚血再灌流 / tenascin-C / matricellular protein / postconditioning / apotosis |
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| Outline of Final Research Achievements |
Tenascin-C expression after myocardial ischemia and reperfusion was investigated using I-125-anti-tneascin-c-antibody (TNC). Autoradiography showed that the uptake peaked 3 days after reperfusion, followed by gradual reduction. Postconditioning at reperfusion suppressed TNC uptake at day 3 and thereafter and attenuated the left ventricular remodeling at 2 months later, suggesting that suppression of tenascin-C expression related to the attenuation of ventricular remodeling.
αvβ3 integrin is expressed both by macrophages and angiogenic endothelial cells. We investigated whether I-125-RGD, which showed high affinity and selectivity for αvβ3, reflects inflammatory process or angiogenesis after myocardial infarction. I-125 RGD accumulated in the infarcted area, and its uptake corresponded closely to micro vessel formation rather than macrophage infiltrations until 2 months after infarction. RGD imaging may be useful for the evaluation of angiogenesis after myocardial infarction.
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