Monitoring Mitochondrial Complex-I Activity Using Novel PET Probe 18F-BCPP-EF Allows Early Detection of Radiotherapy Effect in Murine Squamous Cell Carcinoma
| Project/Area Number |
26461871
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
川口 章 東海大学, 医学部, 教授 (30195052)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | キトコンドリアコンプレックス / MC-I / 放射線治療 / 癌治療効果予測 / ワールブルグ効果 / PET / [18F]FDG / [18F]BCPP-EF / ミトコンドリアコンプレックス / 嫌気的解糖系 / 好気的解糖系 |
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| Outline of Final Research Achievements |
Since ionizing radiation is reported to switch aerobic glycolysis to mitochondrial oxidative phosphorylation, radiotherapeutic efficacy was monitored by the activity of mitochondrial complex I (MC-I), using a new PET probe 18F-BCPP-EF, compare with tumor growth suppression in murine squamous cell carcinoma.
In non-irradiated control mice, there was little uptake of 18F-BCPP-EF. In contrast,Tumor uptake of 18F-BCPP-EF increased significantly early (2, 3 or 4 days) after irradiation with effective doses of irradiation, when tumor volume showed no difference irrespective of radiation dose. The results suggest that 18F-BCPP-EF is a promising “positive” MC-I imaging PET probe for early detection of efficacy of tumor radiotherapy.
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Report
(4 results)
Research Products
(9 results)
