| Project/Area Number |
26461893
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中村 和正 九州大学, 大学病院, 准教授 (20284507)
三森 功士 九州大学, 大学病院, 教授 (50322748)
寺嶋 広太郎 九州大学, 先端医療イノベーションセンター, 助教 (40627676)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 食道癌 / 放射線治療 / マイクロRNA / バイオマーカー / 放射線治療生物学 |
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| Outline of Final Research Achievements |
We previously detected microRNA (miR)-203a as miR involved in radioresistance of esophageal cancer (ESC) cells (Grants-in-Aid for Scientific Research, 20380454).
Purpose of this study is to identify new radiosensitive biomarker and treatment target relating chemoradiation for ESC. The expression of miR-203a was significantly lower in TE1 (Radioresistant ESC cell line) and higher in TE9 (Radiosensitive ESC cell line). On colony formation assay, overexpression of miR-203 impaired resistance to radiation in TE1. MiR-203 was identified as the downregulated miR in radioresistant cell line compared to radiosensitive cell line. Overexpression of miR-203 increased radiosensitivity of ESC cell lines. Our findings indicate that miR-203 can be a target for overcoming the radioresistance of ESC. We also evaluated expression of LASP1 and BMl1 which are target gene of miR-203a, however, we could not obtain the clear result that LASP1 and BMl1 might be radiosensitive gene in ESC cell lines.
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