Basic research on biological visualization of particle track structure using DNA damage repair related factor
| Project/Area Number |
26461902
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | National Institutes for Quantum and Radiological Science and Technology |
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Principal Investigator |
HIRAYAMA Ryoichi 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 放射線障害治療研究部, 主任研究員(定常) (90435701)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 粒子線 / 飛跡構造 / OHラジカル / DNA損傷 / トラック構造 / DNA二本鎖切断 / H2AXのリン酸化 / 放射線の初期過程 |
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| Outline of Final Research Achievements |
I tried that we clarified the relationship between the track structure of the particle and the biological effects. I visualized the truck structure of the particle using a biomarker.
After 30 minutes of irradiation, track size (area) did not change even when LET values increased, but it was found that the track size due to the high-LET particle beam was clearly larger than the other low-LET particles 24 hours after the irradiation. Furthermore, when the OH radical scavenger (DMSO) was treated one hour before irradiation and the track size was quantified after irradiation, the track size tended to decrease due to 1M DMSO addition. These results showed that the increase of the biological effect accompanying the increase of LET does not depend on the size of the particle track. In addition, the involvement of OH radicals was revealed in DSB formation on a surrounding a particle track.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Determination of the relative biological effectiveness and oxygen enhancement ratio for micronuclei formation using high-LET radiation in solid tumor cells: An in vitro and in vivo study2015
Author(s)
Ryoichi Hirayama, Akiko Uzawa, Maki Obara, Nobuhiro Takase, Kana Koda, Masakuni Ozaki, Miho Noguchi, Yoshitaka Matsumoto, Huizi Li, Kei Yamashita, Sachiko Koike, Koichi Ando, Toshiyuki Shirai, Naruhiro Matsufuji and Yoshiya Furusawa
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Journal Title
Mutation Research
Volume: 793
Pages: 41-47
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] THE HIGH BIOLOGICAL EFFECTS OF CARBON-ION BEAMS FOR CELL KILLING AND MICRONUCLEUS FORMATION2014
Author(s)
Ryoichi Hirayama, Akiko Uzawa, Maki Obara, Nobuhiro Takase, Kana Koda, Masakuni Ozaki, Yoshitaka Matsumoto, Toshiyuki Shirai, Yoshiya Furusawa
Organizer
International Conference on Radiation Biology (ICRB2014)
Place of Presentation
NASC Complex, Delhi, India
Year and Date
2014-11-11 – 2014-11-13
Related Report
Invited
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[Presentation] The relative biological effectiveness and oxygen enhancement ratio of low-and high-LET radiations for micronucleus formation in solid tumors2014
Author(s)
Ryoichi Hirayama, Akiko Uzawa, Maki Obara, Nobuhiro Takase, Kana Koda, Ozaki Masakuni, Yoshitaka Matsumoto, Miho Noguchi, Koichi Ando, Toshiyuki Shirai, Yoshiya Furusawa
Organizer
11th International Symposium on Chromosomal Aberrations
Place of Presentation
Aldemar Paradise Mare, Rhodes, Greece
Year and Date
2014-09-12 – 2014-09-14
Related Report
Invited
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