| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The islets are known to vulnerable to oxidative stress because of containing less quantity of anti-oxidant enzymes. Oxidative stress will be given to islets during the process of freeze and thaw. Nrf2 is a key transcriptional factor to regulate the anti-oxidant genes expression. Dimethyl fumarate (DMF) is known as one of Nrf2 activators. The aim of this work is to investigate the cryoprotective effect of dimethyl fumarate (DMF) on rat and human islets.
On rat and human thawed islets treated with DMF, anti-oxidant enzyme mRNA expressions were significantly higher, and the percentage of 7-aminoactinomycin D positive cells were lower than those without DMF. On rat islet transplant to nude mouse model, the levels of blood glucose were significantly lower in DMF group(n=4 in each). On human islets transplanted to nude mice model, the average of the blood glucose in DMF group was low (n=2 in each).
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