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Development of an anti-donor HLA antibody producing humanized mouse model.

Research Project

Project/Area Number 26461911
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General surgery
Research InstitutionHiroshima University

Principal Investigator

Ide Kentaro  広島大学, 病院(医), 病院助教 (50511565)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords抗体関連型拒絶反応 / 抗ドナー特異的HLA抗体 / 脱感作療法 / 慢性抗体関連型拒絶反応 / ヒト化マウス
Outline of Final Research Achievements

Chronic antibody-mediated rejection due to anti-human leukocyte antigen (HLA) antibodies (Abs) is an important problem in organ transplantation. To investigate anti-HLA Ab-producing cells, we developed an anti-HLA Ab-producing humanized mouse model.Severely immunodeficient (NSG) mice were humanized by injection of human peripheral blood mononuclear cells (PBMCs) derived from healthy volunteer. After the appearance of T, B, and myeloid cells in the peripheral blood of the humanized mice, irradiated PBMCs derived from the other healthy volunteer were injected in the mice. We measured anti-HLA antibody levels in sera by using a multiple antigen beads-based assay. Non-DSA anti-HLA-Abs were detected; however, no DSA were detected.
We are now developing humanized mouse model that can produce anti-HLA-Abs and believe that the success will enable further development of in vivo patient-specific therapies, including DSA desensitization.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2017 2016 2015 2014

All Journal Article (5 results) (of which Peer Reviewed: 3 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Book (1 results)

  • [Journal Article] Fc-gamma receptor 3A polymorphism predicts the incidence of urinary tract infection in kidney-transplant recipients.2017

    • Author(s)
      Lalit Kumar Das, Kentaro Ide, Asuka Tanaka, Hiroshi Morimoto, Seiichi Shimizu, Naoki Tanimine, Yuka Tanaka, Hideki Ohdan
    • Journal Title

      Human Immunology

      Volume: 78 Issue: 4 Pages: 357-362

    • DOI

      10.1016/j.humimm.2017.03.006

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Different sensitivity of rituximab-treatment to B-cells between ABO-incompatible kidney and liver transplantation2016

    • Author(s)
      Hiroshi Morimoto, Kentaro Ide, Yuka Tanaka, Kohei Ishiyama, Masahiro Ohira, Hiroyuki Tahara, Tomonori Akita, Junko Tanaka, Hideki Ohdan
    • Journal Title

      Human Immunology

      Volume: 8859 Issue: 6 Pages: 30063-5

    • DOI

      10.1016/j.humimm.2016.04.013

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 腎移植後拒絶反応のメカニズムと未来2016

    • Author(s)
      井手健太郎、大段秀樹
    • Journal Title

      腎臓内科・泌尿器科

      Volume: 3 Pages: 101-105

    • Related Report
      2015 Research-status Report
  • [Journal Article] A phased desensitization protocol with rituximab and bortezomib for highly sensitized kidney transplant candidates2015

    • Author(s)
      Kentaro Ide, Yuka Tanaka, Yu Sasaki, Hiroyuki Tahara, Masahiro Ohira, Kohei Ishiyama, Hirotaka Tashiro, Hideki Ohdan
    • Journal Title

      Transplantation Direct

      Volume: 1 Issue: 5 Pages: 1-6

    • DOI

      10.1097/txd.0000000000000526

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 抗体関連型拒絶反応のメカニズムと制御法2014

    • Author(s)
      井手健太郎、大段秀樹
    • Journal Title

      日本臨床腎移植学会雑誌

      Volume: 2 Pages: 8-14

    • NAID

      40020445587

    • Related Report
      2014 Research-status Report
    • Open Access
  • [Presentation] Current status of desensitization protocol for highly HLA-sensitized kidney transplant candidates2017

    • Author(s)
      Kentaro Ide, Hiroyuki Tahara, Asuka Tanaka, Shuji Akimoto, Sapana Verma, Lalit Kumar Das, Seiichi Shimizu, Masahiro Ohira, Kohei Ishiyama, Yuka Tanaka, Hideki Ohdan
    • Organizer
      第50回日本臨床腎移植学会
    • Place of Presentation
      神戸市
    • Year and Date
      2017-02-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] TLR-MyD88シグナル阻害による異種糖鎖抗原反応性B細胞の制御2016

    • Author(s)
      井手健太郎, 坂井寛,田中友加,大段秀樹
    • Organizer
      第43回日本臓器保存医学会学術集会
    • Place of Presentation
      東京都
    • Year and Date
      2016-11-26
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ミコフェノール酸とMLRによる免疫モニタリング2016

    • Author(s)
      井手健太郎, 田中友加, 田中飛鳥, 大段秀樹
    • Organizer
      第32回腎移植・血管外科研究会
    • Place of Presentation
      姫路市
    • Year and Date
      2016-05-26
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 広島大学における肝および腎移植既存抗体陽性症例に対する基本戦略2015

    • Author(s)
      井手健太郎, 田原裕之, 田中友加, 石山宏平, 大平真裕, 谷峰直樹, 佐伯吉弘, 清水誠一, 坂井寛, 広瀬貴行, 矢野琢也, 石田伸樹, 柳川泉一郎, 田中飛鳥, 大段秀樹
    • Organizer
      第51回日本移植学会総会
    • Place of Presentation
      熊本市
    • Year and Date
      2015-10-01
    • Related Report
      2015 Research-status Report
  • [Presentation] A phased desensitization protocol with rituximab and bortezomib for highly HLA-sensitized kidney transplant candidates.2015

    • Author(s)
      Ide K, Tanaka Y, Sasaki Y, Tahara H, Ohira M, Ishiyama K, Tashiro H, Ohdan H.
    • Organizer
      CAST 2015
    • Place of Presentation
      Singapore
    • Year and Date
      2015-08-23
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 広島大学における前感作肝移植症例に対する脱感作療法2015

    • Author(s)
      井手健太郎, 田原裕之, 田中友加, 石山宏平, 大平真裕, 森本博司, 佐々木由布, 谷峰直樹, 佐伯吉弘, 清水誠一, 坂井寛, 矢野琢也, 石田伸樹, 柳川泉一郎, 広瀬貴行, Das Lalit Kumar, Sapana Verma, 田代裕尊, 大段秀樹
    • Organizer
      第33回日本肝移植研究会
    • Place of Presentation
      神戸市
    • Year and Date
      2015-05-28
    • Related Report
      2015 Research-status Report
  • [Presentation] 前感作腎移植症例に対する脱感作療法の実際2014

    • Author(s)
      井手健太郎 田原裕之 大平真裕 石山宏平 田中友加 森本博司 佐伯吉弘 清水誠一 谷峰直樹 坂井寛 矢野琢也 広瀬貴行 佐々木由布 大段秀樹
    • Organizer
      第50回 日本移植学会
    • Place of Presentation
      東京都
    • Year and Date
      2014-09-10 – 2014-09-12
    • Related Report
      2014 Research-status Report
    • Invited
  • [Book] 腎と透析2014年76巻増刊号 透析・腎移植のすべて2014

    • Author(s)
      井手健太郎、大段秀樹
    • Total Pages
      728
    • Publisher
      東京医学社
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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