Development of an anti-donor HLA antibody producing humanized mouse model.
Project/Area Number |
26461911
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General surgery
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Research Institution | Hiroshima University |
Principal Investigator |
Ide Kentaro 広島大学, 病院(医), 病院助教 (50511565)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 抗体関連型拒絶反応 / 抗ドナー特異的HLA抗体 / 脱感作療法 / 慢性抗体関連型拒絶反応 / ヒト化マウス |
Outline of Final Research Achievements |
Chronic antibody-mediated rejection due to anti-human leukocyte antigen (HLA) antibodies (Abs) is an important problem in organ transplantation. To investigate anti-HLA Ab-producing cells, we developed an anti-HLA Ab-producing humanized mouse model.Severely immunodeficient (NSG) mice were humanized by injection of human peripheral blood mononuclear cells (PBMCs) derived from healthy volunteer. After the appearance of T, B, and myeloid cells in the peripheral blood of the humanized mice, irradiated PBMCs derived from the other healthy volunteer were injected in the mice. We measured anti-HLA antibody levels in sera by using a multiple antigen beads-based assay. Non-DSA anti-HLA-Abs were detected; however, no DSA were detected. We are now developing humanized mouse model that can produce anti-HLA-Abs and believe that the success will enable further development of in vivo patient-specific therapies, including DSA desensitization.
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Report
(4 results)
Research Products
(13 results)
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[Presentation] Current status of desensitization protocol for highly HLA-sensitized kidney transplant candidates2017
Author(s)
Kentaro Ide, Hiroyuki Tahara, Asuka Tanaka, Shuji Akimoto, Sapana Verma, Lalit Kumar Das, Seiichi Shimizu, Masahiro Ohira, Kohei Ishiyama, Yuka Tanaka, Hideki Ohdan
Organizer
第50回日本臨床腎移植学会
Place of Presentation
神戸市
Year and Date
2017-02-15
Related Report
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[Presentation] 広島大学における肝および腎移植既存抗体陽性症例に対する基本戦略2015
Author(s)
井手健太郎, 田原裕之, 田中友加, 石山宏平, 大平真裕, 谷峰直樹, 佐伯吉弘, 清水誠一, 坂井寛, 広瀬貴行, 矢野琢也, 石田伸樹, 柳川泉一郎, 田中飛鳥, 大段秀樹
Organizer
第51回日本移植学会総会
Place of Presentation
熊本市
Year and Date
2015-10-01
Related Report
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[Presentation] 広島大学における前感作肝移植症例に対する脱感作療法2015
Author(s)
井手健太郎, 田原裕之, 田中友加, 石山宏平, 大平真裕, 森本博司, 佐々木由布, 谷峰直樹, 佐伯吉弘, 清水誠一, 坂井寛, 矢野琢也, 石田伸樹, 柳川泉一郎, 広瀬貴行, Das Lalit Kumar, Sapana Verma, 田代裕尊, 大段秀樹
Organizer
第33回日本肝移植研究会
Place of Presentation
神戸市
Year and Date
2015-05-28
Related Report
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