• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Liver regenerative medicine using an amnion and a revival stimulus

Research Project

Project/Area Number 26461917
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General surgery
Research InstitutionNagasaki University

Principal Investigator

TAKATSUKI Mitsuhisa  長崎大学, 医歯薬学総合研究科(医学系), 准教授 (80380939)

Co-Investigator(Kenkyū-buntansha) 江口 晋  長崎大学, 医歯薬学総合研究科(医学系), 教授 (80404218)
三浦 清徳  長崎大学, 医歯薬学総合研究科(医学系), 准教授 (00363490)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords羊膜 / 再生刺激 / アルブミン / 肝細胞 / 培養 / 接着因子
Outline of Final Research Achievements

Because amnion lacks in immunogenicity and includes a lot of mesenchymal stem cells, application to regenerative medicine is expected. Based on the study which showed the differentiation from mesenchymal stem cell in the amnion into myocardial cell, we tried to develop the differentiation from amnion to hepatocyte, with putting the amnion in the liver under regeneration stimulus with 70% partial hepatectomy. However, we could not achieve any successful results. Accordingly, we absolutely changed the strategy that we used the amnion as the culture substrate of the hepatocyte, and we found that compared to other culture substrate including ordinary dish or gel, hepatocyte could maintain the function to produce the albumin in amnion. According to these results, amnion might be good culture substrate not only for hepatocyte, but other various cells.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (4 results)

All 2018 2017

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results)

  • [Journal Article] A Modified Method for Purifying Gallbladder Epithelial Cells Using Fluorescence-activated Cell Sorting.2017

    • Author(s)
      Imamura H, Adachi T, Kitasato A, Sakai Y, Ono S, Hara T, Natsuda K, Soyama A, Hidaka M, Takatsuki M, Kuroki T, Eguchi S.
    • Journal Title

      In Vivo.

      Volume: 31 Pages: 169-173

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ヒト羊膜を用いたラット肝細胞の長期培養2018

    • Author(s)
      前川恭一郎
    • Organizer
      再生医療学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒト羊膜を用いたラット初代肝細胞の長期培養2017

    • Author(s)
      夏田孔史、高槻光寿、堺 祐輔、曽山明彦、足立智彦、大野慎一郎 、原 貴信、岡田怜美、濵田隆志、日高匡章、江口 晋
    • Organizer
      第16回日本再生医療学会総会
    • Place of Presentation
      宮城県仙台市
    • Year and Date
      2017-03-08
    • Related Report
      2016 Research-status Report
  • [Presentation] ラット初代肝細胞は、ヒト羊膜上の培養で長期的に機能維持する2017

    • Author(s)
      夏田孔史、高槻光寿、堺 祐輔、曽山明彦、足立智彦、大野慎一郎 、原 貴信、岡田怜美、濵田隆志、日高匡章、江口 晋
    • Organizer
      第19回日本異種移植研究会
    • Place of Presentation
      京都府京都市
    • Year and Date
      2017-02-25
    • Related Report
      2016 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi