| Project/Area Number |
26461921
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
谷水 直樹 札幌医科大学, 医学部, 准教授 (00333386)
平田 公一 札幌医科大学, 医学部, 教授 (50136959)
三高 俊広 札幌医科大学, 医学部, 教授 (50231618)
石井 雅之 札幌医科大学, 医学部, 研究員 (50643201)
市戸 義久 札幌医科大学, 医学部, 研究員 (80452978)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 肝再生 / 幹細胞 / 細胞バンク / 超過冷却 / 肝細胞移植 / 肝細胞 / 手術 / 腹腔鏡 / 肝不全 / 腹腔鏡下肝切除 / 肝細胞癌 |
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| Outline of Final Research Achievements |
We aimed at establishing the cell harvest method for human hepatocyte with safety and technical issues. We reported an effective method for donor cell harvests with video presentation. We also elucidate clinical values of lactate levels during the cell harvesting. We developed a standard for evaluating postoperative complications. We investigate a cell harvest method regarding anatomical location of the donor cells. BCAA plays an important role to maintain nutritional balance. We also aimed at establishing a banking hepatic stem cells and investigate cell preparation and cell nature. In the research period, we success to separate and culture hepatic stem cells. We tried to protect the method by a patent. Finally, we found an antigen to identify hepatic stem cells and select specific cells for regenerative therapy.
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