| Project/Area Number |
26461925
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
三木 厚 自治医科大学, 医学部, 講師 (20570378)
安田 是和 自治医科大学, 医学部, 客員教授 (40158001)
上本 伸二 京都大学, 医学研究科, 教授 (40252449)
笠原 尚哉 自治医科大学, 医学部, 助教 (50382891)
寺谷 工 自治医科大学, 医学部, 講師 (70373404)
藤本 康弘 京都大学, 医学研究科, 客員研究員 (80335281)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 褐色脂肪細胞 / 表面マーカー / 細胞移植 / 褐色細胞腫 / 脂肪幹細胞 / 肥満治療 / 非アルコール性脂肪肝炎 / 褐色脂肪 / 脂肪肝 / 肥満 / NASH |
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| Outline of Final Research Achievements |
Brown adipocytes play a central role in regulating metabolism and generating metabolic signals. They are also important to maintain metabolic homeostasis by adjusting energy expenditure. However, these cells are not yet well-understood and the tissue-specific presence of uncoupling protein 1 (UCP-1) is a promising lead to gain further understanding. Pheocromocytomas are known to have a tendency to grow into surrounding adipose tissue and become brown adipose tissue. We have successfully collected and cultured these cells from a patient with a pheocromocytoma. There is very little knowledge about Brown adipocytes except for the presence of UCP-1. We performed fluorescence-activated cell sorting combined with a UCP-1 specific aptamer. Cell surface makers were detected using gene-tip analysis and showed CD9, CD177, CD200：positive、and CD40, CD44, CD109: negative.
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