| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We investigated the effects of PPAR-γ agonist on visceral adipose macrophage population and polarisation in cecal ligation and puncture (CLP) mice. Mice were divided to CLP (C)-group, PPARγ agonist-treated CLP (P)-group, or sham (S)-group. mRNA expressions (iNOS for M1, arginase1 and IL-10 for M2, CD163 and F4/80 for mature macrophages) were quantified by real-time RT-PCR. Tissue sections were subjected to immunohistochemical and TUNEL assay. C-group significantly enhanced arginase1, IL-10 and iNOS mRNA expressions as compared with S-group. P-group significantly increased mRNA level of CD163 and F4/80 in C-group. Increased numbers of CD11b/c- and CD163-positive cells as well as apoptotic cells were observed in C-group. These cells were more abundant in P-group. M1/M2 macrophage activation of adipose tissues is induced in C-group. PPAR-γ agonist may accelerate this activation and recruitment of macrophages, and may restrict adipose tissue inflammation by altering macrophage dynamics.
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