Therapeutic effects on cancer stem cells using dendritic-cell vaccine pulsed by cancer cells derived from malignant pleural or peritoneal fluid
| Project/Area Number |
26461934
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | がん幹細胞 / 樹状細胞 / 免疫回避機構 / 癌性腹膜炎 / ワクチン治療 |
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| Outline of Final Research Achievements |
Peritoneal dissemination of gastric cancer stem cells and overcoming immune escape were investigated. In 16 patients with malignant ascites, intraperitoneal free cancer cells were obtained by using cell-free and concentrated ascites rein fusion therapy (CART). CD45-EpCAM+ cancer cells were separated from cancer cells obtained by washing the filtration filters. More than 2.4 x 105 cancer cells were obtained in all of the cases. These data suggest that dendritic cell vaccine can be introduced by using cancer cells in malignant ascites. Cultured gastric cancer cell lines MKN45 and MKN74 were sorted by FACS into CD44+ and CD44- cells. Cytotoxic T cells (CTL) from healthy donor co-cultured with CD44+ gastric cancer cells could kill CD44+ gastric cancer cells as well as that CTL co-cultured with CD44- gastric cancer cells could kill CD44- gastric cancer cells. These results indicate that cancer stem cells may be killed by cancer specific immunotherapy using dendritic cell vaccine.
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Report
(4 results)
Research Products
(5 results)
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[Journal Article] Gastric cancer treated by endoscopic submucosal dissection or endoscopic mucosal resection in Japan from 2004 through 2006: JGCA nationwide registry conducted in 20132017
Author(s)
Tanabe S, Hirabayashi S, Oda I, Ono H, Nashimoto A, Isobe Y, Miyashiro I, Tsujitani S, Seto Y, Fukagawa T, Nunobe S, Furukawa H, Kodera Y, Kaminishi M, Katai H
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Journal Title
Gastric Cancer
Volume: 15
Issue: 5
Pages: 834-842
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Five-year survival analysis of surgically resected gastric cancer cases in Japan: a retrospective analysis of more than 100,000 patients from the nationwide registry of the Japanese Gastric Cancer Association (2001-2007)2017
Author(s)
Katai H, Ishikawa T, Akazawa K, Isobe Y, Miyashiro I, Oda I, Tsujitani S, Ono H, Tanabe S, Fukagawa T, Nunobe S, Kakeji Y, Nashimoto A; Registration Committee of the Japanese Gastric Cancer Association
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Journal Title
Gastric Cancer
Volume: 17
Issue: 1
Pages: 144-154
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Prognostic factors related to add-on dendritic cell vaccines on patients with inoperable pancreatic cancer receiving chemotherapy: a multicenter analysis.2014
Author(s)
Kobayashi M, Shimodaira S, Nagai K, Ogasawara M, Takahashi H, Abe H, Tanii M, Okamoto M, Tsujitani S, Yusa S, Ishidao T, Kishimoto J, Shibamoto Y, Nagaya M, Yonemitsu Y; DC Vaccine Study Group at the Japan Society of Innovative Cell Therapy (J-SICT).
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Journal Title
Cancer Immunol Immunother
Volume: 63
Issue: 8
Pages: 797-806
DOI
Related Report
Peer Reviewed
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