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Novel biomarker for tumor anti-angiogenic therapy based on molecular biology

Research Project

Project/Area Number 26461938
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General surgery
Research InstitutionGunma University

Principal Investigator

Fujii Takaaki  群馬大学, 医学部附属病院, 助教 (40507331)

Co-Investigator(Kenkyū-buntansha) 茂木 晃  群馬大学, 大学院医学系研究科, 准教授 (10323362)
堤 荘一  群馬大学, 医学部附属病院, 講師 (30323356)
森田 廣樹  群馬大学, 医学部附属病院, 医員 (70646794)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords腫瘍血管新生 / リンパ節転移 / VEGF / リンパ節外浸潤 / エリブリン / TGF-β / PDGF-AA / mTOR / FDG-PET / 血管新生因子 / バイオマーカー / 脈管襲侵 / 抗腫瘍効果
Outline of Final Research Achievements

We hypothesized that the mTOR inhibitor-induced anticancer effect is affected by expression of a key angiogenic factor, vascular endothelial growth factor (VEGF). The key observations made in this study can be summarized as follows: 1) administration of rapamycin dose-dependently reduced cell viability of MCF-7, but did not reduce cell viability of HT-29; 2) rapamycin reduced VEGF expression in the culture medium of MCF-7, while rapamycin did not contribute VEGF expression to the culture medium of HT-29; 3) VEGF stimulated cell viability and VEGF inhibition reduced cell viability of MCF-7; and 4) rapamycin dose-dependently restored the cell viability of MCF-7 reduced by rapamycin. These results demonstrate the critical role of VEGF in the action of rapamycin as an anticancer agent on MCF-7. We also revealed that lymph node metastasis and ECI suggest poor prognosis and, especially ECI represent lymphatic aggressive disease and also biological aggressiveness in cases with breast cancer.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (16 results)

All 2016 2015 2014

All Journal Article (13 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 12 results,  Acknowledgement Compliant: 13 results,  Open Access: 4 results) Presentation (3 results)

  • [Journal Article] Clinical Significance of 18F-FDG-PET in Invasive Lobular Carcinoma2016

    • Author(s)
      Fujii T, Yajima R, Kurozumi S, Higuchi T, Obayashi S, Tokiniwa H, Nagaoka R, Takata D, Horiguchi J, Kuwano H
    • Journal Title

      Anticancer Research

      Volume: 6 Pages: 5481-5485

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Implication of 18F-fluorodeoxyglucose uptake by affected lymph nodes in cases with differentiated thyroid cancer2016

    • Author(s)
      Fujii T, Yajima R, Tatsuki H, Kuwano H
    • Journal Title

      Mol Clin Oncol

      Volume: 5 Pages: 247-251

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] mplications of Long-term Indwelling of Tissue Expander in Breast Reconstruction: Risk of Expander Rupturing2016

    • Author(s)
      Fujii T, Yajima R, Kuwano H
    • Journal Title

      Anticancer Res

      Volume: 36 Pages: 4337-4340

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Clinicopathological Features of Cases with Primary Breast Cancer not Identified by 18F-FDG-PET.2016

    • Author(s)
      Fujii T, Yajima R, Tsuboi M, Higuchi T, Obayashi S, Tokiniwa H, Nagaoka R, Takata D, Horiguchi J, Kuwano H
    • Journal Title

      Anticancer Res

      Volume: 36 Pages: 3019-3022

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Prediction of Extracapsular Invasion at Metastatic Sentinel Nodes and Non-sentinel Lymph Nodal Metastases by FDG-PET in Cases with Breast Cancer.2016

    • Author(s)
      Fujii T, Yajima R, Tatsuki H, Kuwano H.
    • Journal Title

      Anticancer Res

      Volume: 36 Pages: 1785-1789

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Implication of 18F-Fluorodeoxyglucose Uptake of Affected Axillary Lymph Nodes in Cases with Breast Cancer.2016

    • Author(s)
      Fujii T, Yajima R, Tatsuki H, Oosone K, Kuwano H
    • Journal Title

      Anticancer Res

      Volume: 36 Pages: 393-397

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Anticancer effect of rapamycin on MCF-7 via downregulation of VEGF expression.2016

    • Author(s)
      Fujii T, Yajima R, Tatsuki H, Oosone K, Kuwano H.
    • Journal Title

      In Vitro Cell Dev Biol Anim.

      Volume: 52 Issue: 1 Pages: 45-48

    • DOI

      10.1007/s11626-015-9944-5

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Significance of lymphatic invasion combined with size of primary tumor for predicting sentinel lymph node metastasis in patients with breast cancer.2015

    • Author(s)
      Fujii T, Yajima R, Tatsuki H, Suto T, Morita H, Tsutsumi S, Kuwano H.
    • Journal Title

      Anticancer Reseach

      Volume: 35 Pages: 3581-3584

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Impact of vascular invasion of a primary tumor as a strong risk factor for disease recurrence in patients with node-positive breast cancer.2015

    • Author(s)
      Fujii T, Yajima R, Morita H, Hirakata T, Miyamoto T, Fujisawa T, Tsutsumi S, Ynagita Y, Iijima M, Kuwano H.
    • Journal Title

      Am Surg

      Volume: 81 Pages: 523-526

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Implication of duration of clinical presentation on tumor progression and short-term recurrence in patients with early breast cancer.2015

    • Author(s)
      Fujii T, Yajima R, Morita H, Suto T, Tatsuki H, Tsutsumi S, Kuwano H.
    • Journal Title

      Mol Clin Oncol

      Volume: 3 Pages: 785-788

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Immediate Tissue-expander Breast Reconstruction Using a Skin Flap with Thick Subcutaneous Tissue: A Preliminary Study on Selective Patients.2015

    • Author(s)
      Fujii T, Yajima R, Tatsuki H, Morita H, Suto T, Tsutsumi S, Kuwano H.
    • Journal Title

      Am Surg.

      Volume: 81

    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Impact of the prognostic value of vascular invasion, but not lymphatic invasion, of the primary tumor in patients with breast cancer.2014

    • Author(s)
      Fujii T, et al.
    • Journal Title

      Anticancer Research

      Volume: 34 Pages: 1255-1259

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Vascular invasion, but not lymphatic invasion, of the primary tumor is a strong prognostic factor in patients with colorectal cancer.2014

    • Author(s)
      Fujii T, et al.
    • Journal Title

      Anticancer Research

      Volume: 34 Pages: 3147-3151

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] FDG-PET偽陰性例の検討:FDG非集積乳癌の臨床病理学的特徴2016

    • Author(s)
      藤井孝明、矢島玲奈、黒住献、樋口徹、尾林紗弥香、時庭英彰、佐藤亜矢子、長岡りん、高他大輔、堀口淳、桑野博行
    • Organizer
      第53回日本癌治療学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2016-10-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] 乳癌腋窩リンパ節転移例における腋窩FDG集積の意義2016

    • Author(s)
      藤井孝明、矢島玲奈、坪井美樹、樋口徹、内田紗弥香、時庭英彰、佐藤亜矢子、長岡りん、高他大輔、堀口淳、桑野博行
    • Organizer
      第24回日本乳癌学会学術総会
    • Place of Presentation
      東京
    • Year and Date
      2016-06-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 乳癌センチネルリンパ節生検におけるFDG-PETによるリンパ節転移、節外浸潤評価2016

    • Author(s)
      藤井孝明、矢島玲奈、大曽根勝也、龍城宏典、桑野博行
    • Organizer
      第116回日本外科学会定期学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2016-04-14
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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