| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Wnt5a was positively expressed in 69 cases (39%) among 178 cases of invasive breast cancer. Wnt5a expression was strongly correlated with being estrogen receptor (ER)-positive (P < 0.001), and Wnt5a was barely expressed in ER-negative breast cancers. Analysis of the relationship between Wnt5a expression and malignancy in exclusively 153 cases of ER-positive breast cancer led to detection of significant correlations with lymph node metastasis (P < 0.001), nuclear grade (P = 0.004), and lymphatic invasion (P = 0.002). Relapse-free survival was shorter in cases of Wnt5a-positive breast cancer compared to Wnt5a-negative breast cancer cases (P = 0.024). MCF7 cells forced to constitutively express Wnt5a showed significantly enhanced migratory capacity as compared to control cells. In order to clarify the mechanism, a DNA microarray analysis was carried out. Activated leukocyte cell adhesion molecule (ALCAM) was identified as an Wnt5a-dependent expression molecule.
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