New strategy for hypothyroidism with use of regenerative medicine.

Research Project

Project/Area Number	26461950
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	General surgery
Research Institution	Nagasaki University
Principal Investigator	MINAMI Shigeki 長崎大学, 医歯薬学総合研究科(医学系), 客員研究員 (90398165)
Co-Investigator(Kenkyū-buntansha)	崎村千香長崎大学, 病院(医学系), 助教 (00567175) 山之内孝彰長崎大学, 医歯薬学総合研究科(医学系), 講師 (10448508)
Project Period (FY)	2014-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords	甲状腺細胞シート / 再生医療 / 甲状腺 / 細胞シート
Outline of Final Research Achievements	1. We successfully fabricated cell sheets with using thyroid cell of resected human thyroid tissues. 2. These cell sheets expressed thyroid cell-specific markers, i. e., TTF1、TG. Furthermore, cultured thyroid cells produced and secreted thyroid hormone in culture medium. 3. Multilayer thyroid cell sheets with fibroblasts (human TIG118) did not show increase in thyroid hormone production compared to monolayer thyroid cell sheets.