Project/Area Number |
26461958
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General surgery
|
Research Institution | Wakayama Medical University |
Principal Investigator |
Yokoyama Shozo 和歌山県立医科大学, 医学部, 講師 (90398462)
|
Co-Investigator(Kenkyū-buntansha) |
堀田 司 和歌山県立医科大学, 医学部, 教授 (50244744)
瀧藤 克也 和歌山県立医科大学, 医学部, 准教授 (00254540)
松田 健司 和歌山県立医科大学, 医学部, 講師 (30398458)
山上 裕機 和歌山県立医科大学, 医学部, 教授 (20191190)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | exosome / CEACAM1 / CEA / 大腸癌 |
Outline of Final Research Achievements |
Enzyme-linked immunosorbent assay (ELISA) for serum exosomal CEACAM1, CEA and CEACAM6 is not common. We established a method to measure serum exosomal CEACAM1, CEA and CEACAM6. In this study, we optimized a method of measuring serum exosomal molecules by ELISA and volume-excluding polymers for exosome isolation. The sample concentration and a solvent for ELISA were important to measure serum exosomal CEACAM1, CEA, and CEACAM6. The thawing temperature of frozen serum before extraction modulates the value of serum exosomal CEA. The samples thawed at 25℃ exhibited a better AUC value, sensitivity, and specificity as well as more correct classification than serum CEA. Serum exosomal CEACAM1 and CEACAM6 are also elevated in patients with distant metastasis. An appropriate concentration of the exosome sample, the solvent for the exosomes, and the thawing temperature of frozen serum before extraction enable accurate measurement of serum exosomal CEA family.
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