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Serum exosomal CEACAM1 as a novel tumor marker for colorectal cancer

Research Project

Project/Area Number 26461958
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General surgery
Research InstitutionWakayama Medical University

Principal Investigator

Yokoyama Shozo  和歌山県立医科大学, 医学部, 講師 (90398462)

Co-Investigator(Kenkyū-buntansha) 堀田 司  和歌山県立医科大学, 医学部, 教授 (50244744)
瀧藤 克也  和歌山県立医科大学, 医学部, 准教授 (00254540)
松田 健司  和歌山県立医科大学, 医学部, 講師 (30398458)
山上 裕機  和歌山県立医科大学, 医学部, 教授 (20191190)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsexosome / CEACAM1 / CEA / 大腸癌
Outline of Final Research Achievements

Enzyme-linked immunosorbent assay (ELISA) for serum exosomal CEACAM1, CEA and CEACAM6 is not common. We established a method to measure serum exosomal CEACAM1, CEA and CEACAM6. In this study, we optimized a method of measuring serum exosomal molecules by ELISA and volume-excluding polymers for exosome isolation. The sample concentration and a solvent for ELISA were important to measure serum exosomal CEACAM1, CEA, and CEACAM6. The thawing temperature of frozen serum before extraction modulates the value of serum exosomal CEA. The samples thawed at 25℃ exhibited a better AUC value, sensitivity, and specificity as well as more correct classification than serum CEA. Serum exosomal CEACAM1 and CEACAM6 are also elevated in patients with distant metastasis. An appropriate concentration of the exosome sample, the solvent for the exosomes, and the thawing temperature of frozen serum before extraction enable accurate measurement of serum exosomal CEA family.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report

Research Products

(12 results)

All 2016 2015 2014

All Journal Article Presentation Patent(Industrial Property Rights)

  • [Journal Article] CEACAM1 and hollow spheroid formation modulate the chemosensitivity of colorectal cancer to 5-fluorouracil.2015

    • Author(s)
      Yamamoto N, Yokoyama S, Ieda J, Mitani Y, Yamaguchi S, Takifuji K, Hotta T, Matsuda K, Watanabe T, Shively JE, Yamaue H.
    • Journal Title

      Cancer Chemotherapy Pharmacology

      Volume: 75 Pages: 421-430

    • DOI

      10.1007/s00280-014-2662-y

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 新たな浸潤形式としての大腸癌浸潤先進部Hollow spheroid2016

    • Author(s)
      横山省三
    • Organizer
      第27回日本消化器癌発生学会
    • Place of Presentation
      鹿児島
    • Year and Date
      2016-09-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] P4H9 detecting molecule in respnse to CEACAM1 in colorectal cancer2016

    • Author(s)
      Shozo Yokoyama
    • Organizer
      International CEA Symposium
    • Place of Presentation
      コーク、アイルランド
    • Year and Date
      2016-08-15
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cancer cell cluster with Hollow spheroid formation modulates chemosensitivity of colorectal cancer cells -Three-Dimension culture for evaluation of chemosensitivity-.2016

    • Author(s)
      Shozo Yokoyama
    • Organizer
      ASCO Annual meeting
    • Place of Presentation
      シカゴ、USA
    • Year and Date
      2016-06-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CEACAM1細胞内ドメインisoform balanceと大腸癌の抗癌剤感受性と腫瘍形成能2016

    • Author(s)
      横山省三
    • Organizer
      第116回日本外科学会
    • Place of Presentation
      大阪
    • Year and Date
      2016-04-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Expression of P4H9 detecting molecule on spindle shaped fibroblasts indicates malignant phenotype of colorectal cancer2016

    • Author(s)
      横山省三
    • Organizer
      ASCO-GI
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2016-01-23
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] CEACAM1細胞内ドメインisoform balanceとHollow spheroid formatonによる大腸癌細胞の5FUに対する感受性変化2015

    • Author(s)
      横山省三
    • Organizer
      日本癌学会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-10-09
    • Related Report
      2015 Research-status Report
  • [Presentation] 大腸癌の抗癌剤感受性と腫瘍形成能におけるCEACAM1の役割2015

    • Author(s)
      横山省三
    • Organizer
      日本がん転移学会
    • Place of Presentation
      大阪
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
  • [Presentation] CEACAM1 cytoplasmic domain isoform変化による肝細胞癌浸潤能の増強2014

    • Author(s)
      横山省三
    • Organizer
      日本肝胆膵外科学会
    • Place of Presentation
      和歌山
    • Year and Date
      2014-06-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 大腸癌浸潤先進部間質における間葉上皮転換2014

    • Author(s)
      横山省三
    • Organizer
      癌免疫外科研究会
    • Place of Presentation
      大阪
    • Year and Date
      2014-05-23
    • Related Report
      2014 Research-status Report
  • [Presentation] 肝細胞癌浸潤におけるCEACAM1 cytoplasmic domain isoformバランスの意義2014

    • Author(s)
      横山省三
    • Organizer
      日本外科学会
    • Place of Presentation
      京都
    • Year and Date
      2014-04-05
    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] エクソソームの膜に存在するタンパク質の検出方法2016

    • Inventor(s)
      横山省三
    • Industrial Property Rights Holder
      横山省三
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-158985
    • Filing Date
      2016-08-12
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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