Functional analysis of tumor suppressor gene BAP1, NPM1 complex

• Project/Area Number: 26461962
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: Nishikawa Hiroyuki 聖マリアンナ医科大学, 医学研究科, 研究技術員 (90387077)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 癌抑制遺伝子 / DNA修復 / 脱ユビキチン化

Outline of Final Research Achievements

As a result of research, the following was found out.
NPM 1 binds to BAP 1 in the region of 1 - 122
BAP１, NPM 1 complex controls HCF 1 pathway, controls chromatin remodeling and involved in apoptosis induction.

Research Products

• [Journal Article] Functional Link between BRCA1 and BAP1 through Histone H2A, Heterochromatin and DNA Damage Response. (2016)
  • Author(s): Fukuda T, Tsuruga T, Kuroda T, Nishikawa H, Ohta T.
  • Journal Title: Curr Cancer Drug Targets. Volume: 16(2) Issue: 2 Pages: 101-9
  • DOI: 10.2174/1568009615666151030102427
  • Peer Reviewed
• [Journal Article] Liganded ERα Stimulates the E3 Ubiquitin Ligase Activity of UBE3C to Facilitate Cell Proliferation. (2015)
  • Author(s): Okada M, Ohtake F, Nishikawa H, Wu W, Saeki Y, Takana K, Ohta T.
  • Journal Title: Mol Endocrinol. Volume: 29(11) Pages: 1646-57
  • Peer Reviewed
• [Journal Article] Interaction of BARD1 and HP1 Is Required for BRCA1 Retention at Sites of DNA Damage. (2015)
  • Author(s): Wu W, Nishikawa H, Fukuda T, Vittal V, Asano M, Miyoshi Y, Klevit RE, Ohta T.
  • Journal Title: Cancer Research Volume: 75(7) Pages: 1311-1321
  • Peer Reviewed
• [Journal Article] Ubiquitin acetylation inhibits polyubiquitin chain elongation. (2015)
  • Author(s): Ohtake F, Saeki Y, Sakamoto K, Ohtake K, Nishikawa H, Tsuchiya H, Ohta T, Tanaka K, Kanno J.
  • Journal Title: EMBO Reports Volume: 16(2) Pages: 192-201
  • Peer Reviewed