Development of biomarkers for precision medicine in early gastric cancer

• Project/Area Number: 26461973
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Gifu University
• Principal Investigator: YOSHIDA KAZUHIRO 岐阜大学, 大学院医学系研究科, 教授 (50230727)
• Co-Investigator(Kenkyū-buntansha): 円谷 彰 横浜市立大学, 附属市民総合医療センター, 准教授 (00236936) ; 大島 貴 横浜市立大学, 医学部, 准教授 (10448665)
• Research Collaborator: SAKAMOTO JUNICHI ; TANAKA SHIRO ; MIYAGI YOHEI ; TAN Patrick
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 胃癌 / 5FU / タキサン / 予後因子 / 効果予測因子 / OPRT / TS / NT / TK / 耐性 / 胃癌補助化学療法 / 胃癌個別化療法 / バイオマーカー

Outline of Final Research Achievements

The purpose of this study is to clarify the prognostic and predictive factor for gastric cancer using the samples of the patients enrolled in the SAMIT trial, which investigating the efficacy of the postoperative chemotherapy for gastric cancer. Ohshima et al. extracted RNA from the samples and performed the pattern analysis of mRNA expression and the quantitative RT-PCR for 101 genes. Yoshida et al. analyzed the mechanism of the resistance to 5FU using gastric cancer cell lines, and found that the reduction of F-deoxyuridine monophosphate by nucleotidase was one of the mechanism for the resistance to 5FU in gastric cancer.

Research Products

• [Journal Article] Adjuvant capecitabine plus oxaliplatin after D2 gastrectomy in Japanese patients with gastric cancer: a phase II study (2017)
  • Author(s): Fuse N, Bando H, Chin K, Ito S, Yoshikawa T, Tsuburaya A, Terashima M, Kawashima Y, Fukunaga T, Gotoh M, Emi Y, Yoshida K, Oki E, Takahashi S, Kuriki H, Sato K, Sasako M
  • Journal Title: Gastric Cancer Volume: 20 Pages: 332-340
  • DOI: 10.1007/s10120-016-0606-4
  • Peer Reviewed / Open Access
• [Journal Article] Is conversion therapy possible in stage IV gastric cancer: the proposal of new biological categories of classification (2016)
  • Author(s): Yoshida K,Yamaguchi K, Okumura N, Tanahashi T, Kodera Y
  • Journal Title: Gastric Cancer Volume: 19 Pages: 329-338
  • DOI: 10.1007/s10120-015-0575-z
  • Peer Reviewed / Open Access
• [Journal Article] Clinicopathological factors associated with HER2 status in gastric cancer: results from a prospective multicenter observational cohort study in a Japanese population (JFMC44-1101) (2016)
  • Author(s): Matsusaka S, Nashinmoto A, Nishikawa K, Miki A, Miwa H, Yamaguchi K, Yoshikawa T, Ochiai A, Morita S, Sano T, Kodera Y, Kakeji Y, Sakamoto J, Saji S, Yoshida K
  • Journal Title: Gastric Cancer Volume: 19 Pages: 839-851
  • DOI: 10.1007/s10120-015-0518-8
  • Peer Reviewed / Open Access
• [Journal Article] A Randomized Phase III Trial Comparing S-1 Versus UFT as Adjuvant Chemotherapy for Stage II/III Rectal Cancer. (JFMC35-C1: ACTS-RC) (2016)
  • Author(s): Oki E, Murata A, Yoshida K, Maeda K, Ikejiri K, Munemoto Y, Sasaki K, Matsuda C, Kotake M, Suenaga T, Matsuda M, Emi Y, Kakeji Y, Baba H, Hamada C, Saji S, Maehara Y
  • Journal Title: Annals of Oncology Volume: 27 Pages: 1266-1272
  • DOI: 10.1093/annonc/mdw162
  • Peer Reviewed / Open Access
• [Journal Article] Overexpression of KIF11 in Gastric Cancer with Intestinal Mucin Phenotype (2016)
  • Author(s): Imai T, Oue N, Nishioka M, Mukai S, Oshima T, Sakamoto N, Sentani K, Matsusaki K, Yoshida K, Yasui W
  • Journal Title: Pathobiology Volume: 84 Pages: 16-24
  • DOI: org/10.1159/000447303
  • Peer Reviewed / Open Access
• [Journal Article] Induction of KIFC1 expression in gastric cancer spheroids (2016)
  • Author(s): Oue N, Mukai S, Imai T, Pham TT, Oshima T, Sentani K, Sakamoto N, Yoshida K, Yasui W
  • Journal Title: Oncology Reports Volume: 36 Pages: 349-355
  • DOI: 10.3892/or.2016.4781
  • Peer Reviewed / Open Access
• [Journal Article] Conversion therapy for stage IV gastric cancer -the present and future (2016)
  • Author(s): Yamaguchi K, Yoshida K, Tanaka Y, Matsuhashi N, Tanahashi T, Takahashi T
  • Journal Title: Translational Gastroenterology and Hepatology Volume: 1 Pages: 50
  • DOI: 10.21037/tgh.2016.05.12
  • Peer Reviewed / Open Access
• [Journal Article] The Significant Role of Cyclin D1 in the Synergistic Growth-inhibitory Effect of Combined Therapy of Vandetanib with 5-Fluorouracil for Gastric Cancer (2016)
  • Author(s): Yawata K, Osada S, Tanahashi T, Matsui S, Sasaki Y, Tanaka Y, Okumura N, Matsuhashi N, Takahashi T, Yamaguchi K, Yoshida K
  • Journal Title: Anticancer Research Volume: 36 Pages: 5215-5226
  • DOI: 10.21873/anticanres.11092
  • Peer Reviewed / Open Access
• [Journal Article] MiR-133b inhibits growth of human gastric cancer cells by silencing PKM-splicer PTBP1 (2016)
  • Author(s): Sugiyama T, Taniguchi K, Matsuhashi N, Tajirika T, Futamura M, Takai T, Akao Y, Yoshida K
  • Journal Title: Cancer Science Volume: 107 Pages: 1767-1775
  • DOI: 10.1111/cas.13091
  • Peer Reviewed / Open Access
• [Journal Article] Sequential paclitaxel followed by tegafur and uracil (UFT) or S-1 versus UFT or S-1 monotherapy as adjuvant chemotherapy for T4a/b gastric cancer (SAMIT): a phase 3 factorial randomised controlled trial (2014)
  • Author(s): Tsuburaya A, Yoshida K, Kobayashi M, Yoshino S, Takahashi M, Takiguchi N, Tanabe K,Takahashi N, Imamura H, Tatsumoto N, Hara H, Nishikawa K, Fukushima R, Nozaki I, Kojima H, Miyashita Y, Oba K, Buyse M, Morita M, Sakamoto J
  • Journal Title: Lancet Oncology Volume: 15 Pages: 886-893
  • DOI: http://dx.doi.org/10.1016/S1470-2045(14)70025-7
  • Peer Reviewed / Open Access
• [Presentation] 5FU-resistance caused by reduced FdUMP, and its reversal using deoxyuridine (2017)
  • Author(s): Ryutaro Mori, Toshiyuki Tanahashi, Kazuya Yamaguchi, Kazuhiro Yoshida
  • Organizer: 第89回日本胃癌学会総会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2017-03-08 – 2017-03-10
• [Presentation] FdUMPの解毒による5FU耐性とdeoxyuridineによる耐性解除 (2016)
  • Author(s): 森龍太郎、棚橋利行、山口和也、二村学、吉田和弘
  • Organizer: 第54回日本癌治療学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-20 – 2016-10-22
• [Presentation] FdUMPの解毒による5FU耐性 (2016)
  • Author(s): 森龍太郎、棚橋利行、山口和也、二村学、吉田和弘
  • Organizer: 第27回日本消化器癌発生学会総会
  • Place of Presentation: 城山観光ホテル
  • Year and Date: 2016-09-15 – 2016-09-16
• [Presentation] 5FU耐性胃癌細胞株に対するdUMPの耐性減弱作用 (2016)
  • Author(s): 森龍太郎、棚橋利行、奥村直樹、山口和也、吉田和弘
  • Organizer: 第71回日本消化器外科学会総会
  • Place of Presentation: あわぎんホール、アスティ徳島
  • Year and Date: 2016-07-14 – 2016-07-16
• [Presentation] 5FU耐性胃癌細胞株に対するdUMPの耐性減弱作用 (2016)
  • Author(s): 森龍太郎、土屋博、棚橋利行、奥村直樹、山口和也、吉田和弘
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 大阪国際会議場・リーガロイヤルホテル大阪
  • Year and Date: 2016-04-14 – 2016-04-16
• [Presentation] 5FU耐性胃癌細胞株に認められたFdUMPの解毒による5FU耐性 (2016)
  • Author(s): 森龍太郎、棚橋利行、奥村直樹、山口和也、吉田和弘
  • Organizer: 第49回制癌剤適応研究会
  • Place of Presentation: 福島県会津若松市 会津東山温泉 御宿東鳳
  • Year and Date: 2016-03-25 – 2016-03-25
• [Presentation] Deoxyuridine monophosphate reverses resistance to 5FU in 5FU-resistant gastric cancer (2015)
  • Author(s): Mori R, Tanahashi T, Okumura N, Yamaguchi K, Futamura M, Yoshida K.
  • Organizer: ESMO Asia 2015
  • Place of Presentation: Singapore Suntec Singapore Convention & Exhibition Centre
  • Year and Date: 2015-12-19 – 2015-12-19
  • Int'l Joint Research
• [Presentation] 5FU耐性胃癌細胞株に対するdUMPの耐性減弱作用 (2015)
  • Author(s): 森龍太郎、棚橋利行、奥村直樹、山口和也、二村学、吉田和弘
  • Organizer: 第26回日本消化器癌発生学会
  • Place of Presentation: 鳥取県米子市 米子全日空ホテル
  • Year and Date: 2015-11-20 – 2015-11-20
• [Presentation] 5FU耐性胃癌細胞株に対するdUMPの耐性減弱作用 (2015)
  • Author(s): 森龍太郎、棚橋利行、奥村直樹、山口和也、二村学、吉田和弘
  • Organizer: 第53回日本癌治療学会
  • Place of Presentation: 京都市 国立京都国際会館・グランドプリンスホテル京都
  • Year and Date: 2015-10-30 – 2015-10-30
• [Presentation] 5FU耐性胃癌細胞株に対するdUMPの耐性減弱作用 (2015)
  • Author(s): 森龍太郎、棚橋利行、奥村直樹、山口和也、二村学、吉田和弘
  • Organizer: 第24回日本癌病態治療研究会
  • Place of Presentation: 栃木県日光市 日光千姫物語
  • Year and Date: 2015-06-25 – 2015-06-25