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Label-free imaging identification of cells using quantitative phase microscopy for negative selection of Circulating Tumor Cells

Research Project

Project/Area Number 26461974
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

Kawabata Toshiki  浜松医科大学, 医学部附属病院, 診療助教 (90402289)

Co-Investigator(Kenkyū-buntansha) 今野 弘之  浜松医科大学, 医学部, 学長 (00138033)
平松 良浩  浜松医科大学, 医学部, 助教 (00397390)
菊池 寛利  浜松医科大学, 医学部, 助教 (70397389)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords血中循環腫瘍細胞 / 定量位相顕微鏡 / 癌 / 診断
Outline of Final Research Achievements

We observed WBCs and five cell line cells flowing in the chamber by quantitative phase microscopy (QPM) that provides quantitative morphological information with high contrast without staining. Then, we extracted certain features from the obtained images as training data for pattern recognition, and created an algorithm to differentiate WBCs from cell lines. The obtained algorithm successfully differentiated WBCs from cell lines (AUC=0.99). Our label-free, non-cytotoxic cell recognition method based on the features of QPM images has the potential to serve as a novel method for cell recognition and negative selection of circulating tumor cells (CTCs).

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2016 2015 2014

All Presentation (11 results) (of which Int'l Joint Research: 4 results)

  • [Presentation] Label-free imaging identification of white blood cells using quantitative phase microscope for negative selection of circulating tumor cells2016

    • Author(s)
      尾﨑裕介
    • Organizer
      10th ISMRC International Symposium on Minimal Residual Cancer (ISMRC): Liquid Biopsy in Cancer Diagnostics and Treatment
    • Place of Presentation
      Hamburg, Germany
    • Year and Date
      2016-03-19
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 定量位相顕微鏡と画像認証システムを用いた新たな血中循環腫瘍細胞(CTC)検出手法の試み2016

    • Author(s)
      尾﨑裕介
    • Organizer
      第116回 日本外科学会定期学術集会
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2016 Annual Research Report
  • [Presentation] Label-free imaging identification of WBCs on the features of Quantitative Phase Microscope Images for negative selection of CTCs2016

    • Author(s)
      尾﨑裕介
    • Organizer
      American Association for Cancer Research Annual Meeting 2016
    • Place of Presentation
      New Orleans, USA
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 定量位相顕微鏡と画像認証システムを用いた新たな血中循環腫瘍細胞検出の試み2016

    • Author(s)
      尾﨑裕介
    • Organizer
      第20回 日本がん分子標的治療学会学術集会
    • Place of Presentation
      別府国際コンベンションセンター
    • Related Report
      2016 Annual Research Report
  • [Presentation] Label free identification of cells based on quantitative phase microscopy and machine learning2016

    • Author(s)
      尾﨑裕介
    • Organizer
      第71回 日本消化器外科学会総会
    • Place of Presentation
      徳島 あわぎんホール
    • Related Report
      2016 Annual Research Report
  • [Presentation] Label-free imaging identification of cells using quantitative phase microscopy for negative selection of CTCs2016

    • Author(s)
      尾﨑裕介
    • Organizer
      第75回 日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2016 Annual Research Report
  • [Presentation] Label-free imaging identification of white cells using quantitative phase microscopy for negative selection of CTCs2016

    • Author(s)
      尾﨑裕介
    • Organizer
      40th World Congress of the International College of Surgeons
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Label-free identification of cells using quantitative phase microscope for negative selection of CTCs2015

    • Author(s)
      尾﨑裕介
    • Organizer
      第74回 日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-10-10
    • Related Report
      2015 Research-status Report
  • [Presentation] Label free identification of cells based on quantitative phase microscopy and machine learning2015

    • Author(s)
      尾﨑裕介
    • Organizer
      第70回 日本消化器外科学会総会
    • Place of Presentation
      浜松
    • Year and Date
      2015-07-15
    • Related Report
      2015 Research-status Report
  • [Presentation] Label-free imaging identification of white blood cells using quantitative phase microscope for negative selection of circulating tumor cells2015

    • Author(s)
      尾﨑裕介
    • Organizer
      American Association for Cancer Research Annual Meeting 2015
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2015-04-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Label free imaging identification of white blood cells in cell-line cells based on object recognition using quantitative phase microscopy and machine learning: non-cytotoxic, marker-free isolation of circulating tumor cells2014

    • Author(s)
      Yusuke Ozaki
    • Organizer
      2nd International Symposium on Advances in Circulating Tumor Cells (ACTC)
    • Place of Presentation
      Crete, Greece
    • Year and Date
      2014-10-10
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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