Development of anti-metastasis therapy in the inflammation-related gastric cancer progression

• Project/Area Number: 26461978
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Okayama University
• Principal Investigator: NISHIZAKI MASAHIKO 岡山大学, 大学病院, 講師 (30379789)
• Co-Investigator(Kenkyū-buntansha): 田澤 大 岡山大学, 大学病院, 准教授 (90415513)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 胃癌 / 慢性炎症 / 微小環境 / 悪性化進展 / p53

Outline of Final Research Achievements

Chronic inflammation-related inflammatory microenvironment is a risk factor in gastric cancer development. In this study, we showed the promoting effect of inflammatory microenvironment, such as stromal fibroblast and macrophage, in gastric cancer progression. Moreover, we confirmed that a tumor-specific replication-competent oncolytic adenovirus OBP-702 that induces tumor suppressor p53 gene had a therapeutic potential to induce profound anti-tumor effect against intraperitoneal metastasis of gastric cancer.

Research Products

• [Journal Article] Double-flap technique as an antireflux procedure in esophagogastrostomy after proximal gastrectomy (2016)
  • Author(s): Kuroda S, Nishizaki M, Kikuchi S, Noma K, Tanabe S, Kagawa S, Shirakawa Y, Fujiwara T
  • Journal Title: J Am Coll Surg Volume: 223 Issue: 2 Pages: e7-e13
  • DOI: 10.1016/j.jamcollsurg.2016.04.041
  • Peer Reviewed
• [Journal Article] Recurrence after endoscopic curative resection of mucosal gastric cancer associated with an adjacent neoplastic precursor lesion (2016)
  • Author(s): Kikuchi S, Kagawa S, Ohara T, Kubota T, Kuwada K, Kagawa T, Kuroda S, Shirakawa Y, Nishizaki M, Fujiwara T
  • Journal Title: Acta Med Okayama Volume: 70 Pages: 213-216
  • NAID: 120005770690
  • Peer Reviewed
• [Journal Article] Nonexposure laparoscopic and endoscopic cooperative surgery (closed laparoscopic and endoscopic cooperative surgery) for gastric submucosal tumor (2016)
  • Author(s): Kikuchi S, Nishizaki M, Kuroda S, Tanabe S, Noma K, Kagawa S, Shirakawa Y, Kato H, Okada H, Fujiwara T
  • Journal Title: Gastric Cancer Volume: 20 Issue: 3 Pages: 553-557
  • DOI: 10.1007/s10120-016-0641-1
  • NAID: 40021185849
  • Peer Reviewed
• [Journal Article] Biological ablation of sentinel lymph node metastasis in submucosally invaded early gastrointestinal cancer. (2015)
  • Author(s): Kikuchi S, et al.
  • Journal Title: Molecular Therapy Volume: 23 Issue: 3 Pages: 501-509
  • DOI: 10.1038/mt.2014.244
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Biological ablation of sentinel lymph node metastasis in submucosally invaded early gastrointestinal cancer. (2015)
  • Author(s): Kikuchi S, Kishimoto H, Tazawa H, Hashimoto Y, Kuroda S, Nishizaki M, Nagasaka T, Shirakawa Y, Kagawa S, Urata Y, Hoffman RM, Fujiwara T.
  • Journal Title: Mol Ther. Volume: 23(3) Issue: 4 Pages: 501-509
  • DOI: 10.1136/gutjnl-2014-306957
  • NAID: 120005537574
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Feasibility of oral administration of S-1 as adjuvant chemotherapy in gastric cancer: 4-week S-1 administration followed by 2-week rest vs. 2-week administration followed by 1-week rest (2015)
  • Author(s): Yamatsuji T, Fujiwara Y, Matsumoto H, Hato S, Namikawa T, Hanazaki K, Takaoka M, Hayashi J, Shigemitsu K, Yoshida K, Urakami A, Uno F, Nishizaki M, Kagawa S, Ninomiya M, Fujiwara T, Hirai T, Nakamura M, Haisa M, Naomoto Y
  • Journal Title: Mol Clin Oncol Volume: 3 Pages: 527-532
  • Peer Reviewed
• [Journal Article] Strategic approach to concurrent aberrant left gastric vein and aberrant left hepatic artery in laparoscopic distal gastrectomy for early gastric cancer: A case report (2015)
  • Author(s): Kuwada K, Kuroda S, Kikuchi S, Hori N, Kubota T, Nishizaki M, Kagawa S, Fujiwara T
  • Journal Title: Asian J Endosc Surg Volume: 8 Issue: 4 Pages: 454-456
  • DOI: 10.1111/ases.12203
  • Peer Reviewed
• [Journal Article] Pathological complete response in a case of advanced gastric cancer after neoadjuvant chemotherapy (2014)
  • Author(s): Kikuchi S, Kagawa S, Kubota T, Kuwada K, Kuroda S, Nishizaki M, Tanaka T, Fujiwara T
  • Journal Title: Gan To Kagaku Ryoho Volume: 41 Pages: 2282-2284
  • Peer Reviewed
• [Journal Article] Two cases of stage IV gastric cancer responding to chemotherapy and leading to pathological complete response (2014)
  • Author(s): Kubota T, Kagawa S, Kikuchi S, Kuroda S, Nishizaki M, Fujiwara T
  • Journal Title: Gan To Kagaku Ryoho Volume: 41 Pages: 2308-2310
  • Peer Reviewed
• [Journal Article] Establishment of a non-invasive semi-quantitative bioluminescent imaging method for monitoring of an orthotopic esophageal cancer mouse model. (2014)
  • Author(s): Kuroda S, et al.
  • Journal Title: PLoS One Volume: 9 Issue: 12 Pages: e114562-e114562
  • DOI: 10.1371/journal.pone.0114562
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] p53活性化能を有するテロメラーゼ標的型ウイルス療法による腹腔内胃癌微小環境の浄化作用 (2016)
  • Author(s): 田澤 大、堀 直人、國府島 健、谷本光隆、家田偉史、渡邉めぐみ、黒田新士、西崎正彦、浦田泰生、香川俊輔、藤原俊義
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2016-10-06
• [Presentation] 炎症性微小環境によって誘導されるEMTの蛍光生細胞イメージングシステム (2016)
  • Author(s): 家田偉史、田澤 大、菊地覚次、黒田新士、大原利章、野間和広、岸本浩行、永坂岳司、西崎正彦、香川俊輔、今村健志、藤原俊義
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2016-10-06
• [Presentation] Inhibition of peritoneal dissemination of scirrhous gastric cancer by oncolytic adenovirus-mediated activation of tumor suppressor p53 and suppression of oncogenic receptor tyrosine kinase (2016)
  • Author(s): Tazawa H, Hori N, Koujima T, Kuwada K, Tanimoto T, Kuroda S, Nishizaki M, Urata Y, Kagawa S, Fujiwara T
  • Organizer: 第22回日本遺伝子細胞治療学会
  • Place of Presentation: 東京
  • Year and Date: 2016-07-28
• [Presentation] A novel imaging system for inflammation-induced epithelial-mesenchymal transition in colorectal cancers (2016)
  • Author(s): Ieda T, Tazawa H, Kikuchi S, Kuroda S, Ohara T, Noma K, Kishimoto H, Nagasaka T, Nishizaki M, Kagawa S, Fujiwara T
  • Organizer: 107th Annual Meeting of the American Association for Cancer Research
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] スキルス胃癌細胞に対する腫瘍特異的p53遺伝子治療の前臨床評価 (2015)
  • Author(s): 堀 直人、田澤 大、西崎正彦、渡邉めぐみ、田村周太、國府島 健、黒田新士、浦田泰生、香川俊輔、藤原俊義
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-10
• [Presentation] 分化型胃癌細胞による癌関連線維芽細胞の活性化機構 (2015)
  • Author(s): 李 云成、田澤 大、西崎正彦、橋本悠里、堀 直人、勝部亮一、黒田新士、野間和広、香川俊輔、藤原俊義
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-10
• [Presentation] Preclinical evaluation of telomerase-specific p53 tumor suppressor gene overexpression in human scirrhous gastric cancer cells with different p53 status (2015)
  • Author(s): Hori N, Tazawa H, Nishizaki M, Watanabe M, Tamura S, Koujima T, Kuroda S, Urata Y, Kagawa S, Fujiwara T
  • Organizer: 第21回日本遺伝子治療学会
  • Place of Presentation: 大阪
  • Year and Date: 2015-07-25
• [Presentation] Preclinical study of telomerase-specific p53 tumor suppressor overexpression in human scirrhous gastric cancer cells with different p53 status (2015)
  • Author(s): Hori N, Tazawa H, Nishizaki M, Kikuchi S, Yano S, Ishida M, Watanabe M, Urata Y, Kagawa S, Fujiwara T
  • Organizer: 106th Annual Meeting of the American Association for Cancer Research
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2015-04-22
  • Int'l Joint Research
• [Presentation] Differentiated gastric cancer cells have a potential to induce cancer-associated fibroblasts (2015)
  • Author(s): Li Y, Tazawa H, Nishizaki M, Hashimoto Y, Hori N, Katsube R, Kuroda S, Noma K, Kagawa S, Fujiwara T
  • Organizer: 106th Annual Meeting of the American Association for Cancer Research
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2015-04-20
  • Int'l Joint Research
• [Presentation] 蛍光発現ウイルスを用いた腹腔内がん細胞の検出技術の開発 (2014)
  • Author(s): 渡邉めぐみ、香川俊輔、石田道拡、橋本悠里、堀 直人、菊地覚次、黒田新士、岸本浩行、西崎正彦、田澤 大、浦田泰生、藤原俊義
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-26
• [Presentation] ヒトスキルス胃癌細胞に対する腫瘍融解アデノウイルスの抗腫瘍効果 (2014)
  • Author(s): 堀 直人、田澤 大、西崎正彦、菊地覚次、矢野修也、石田道拡、渡邉めぐみ、浦田泰生、香川俊輔、藤原俊義
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-26
• [Presentation] Virus-guided fluorescence imaging of intraperitoneal gastric cancer cells as an alternative to cytology (2014)
  • Author(s): Watanabe M, Kagawa S, Ishida M, Hori N, Kikuchi S, Kuroda S, Kishimoto H, Nishizaki M, Tazawa H, Urata Y, Fujiwara T
  • Organizer: 第20回日本遺伝子治療学会
  • Place of Presentation: 東京
  • Year and Date: 2014-08-08
• [Presentation] Virus-guided fluorescence imaging of intraperitoneal free gastric cancer cells as a potential clinical biomarker (2014)
  • Author(s): Watanabe M, Kagawa S, Ishida M, Hori N, Kikuchi S, Kuroda S, Kishimoto H, Nishizaki M, Tazawa H, Fujiwara T
  • Organizer: 105th Annual Meeting of the American Association for Cancer Research
  • Place of Presentation: San Diego, USA
  • Year and Date: 2014-04-08
• [Book] Advances in Medicine and Biology. Volume 110 (2017)
  • Author(s): Tazawa H, Kagawa S, Shigeyasu K, Fujiwara T
  • Total Pages: 226
  • Publisher: Nova Science Publishers
• [Book] Recent Results in Cancer Research (Current Strategies in Cancer Gene Therapy) (2016)
  • Author(s): Tazawa H, Kagawa S, Fujiwara T
  • Total Pages: 121
  • Publisher: Springer International Publishing