Development of risk stratification kit and small molecular anticancer drug by biomarker of gastric cancer

• Project/Area Number: 26461984
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Yokohama City University
• Principal Investigator: OSHIMA Takashi 横浜市立大学, 医学部, 准教授 (10448665)
• Research Collaborator: NEAMATI Nouri
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 胃癌 / バイオマーカー / 新規ターゲット / リスク層別化キット / 低分子抗癌剤

Outline of Final Research Achievements

We searched for the candidates of recurrence risk stratification markers of Stage II/III gastric cancer by quantitative PCR method and exploratory proteomics. 13 genes were identified by the quantitative PCR method, and 4 proteins were identified by exploratory proteomics. Then we examined the expression of these marker candidates by immunostaining using tissue microarray, it was possible to stratify the risk of recurrence with the combination of SPARC, EZH2, ERBB2, p53.
In development of small molecular anticancer drugs targeting KIAA 1199, it was narrowed down to 12 kinds of drugs due to the antitumor effect on gastric cancer cells. We then examinied the effect of combination with 5-FU on gastric cancer by Chou and Talaly method, synergistic effect was confirmed in one drug.We are planning to conduct animal experiments using this drug.

Research Products

• [Journal Article] Clinical significance of platelet-derived growth factor receptor-β gene expression in stage II/III gastric cancer with S-1 adjuvant chemotherapy. (2017)
  • Author(s): Higuchi A, Oshima T, Yoshihara K, Sakamaki K, Aoyama T, Suganuma N, Yamamoto N, Sato T, Cho H, Shiozawa M, Yoshikawa T, Rino Y, Kunisaki C, Imada T, Masuda M.
  • Journal Title: Oncology letters Volume: 13 Issue: 2 Pages: 905-911
  • DOI: 10.3892/ol.2016.5494
  • Peer Reviewed / Open Access
• [Journal Article] Clinical significance of SPARC gene expression in patients with stage II/III gastric cancer after curative resection and adjuvant chemotherapy with S-1. (2017)
  • Author(s): Suzuki Y, Oshima T, Yoshihara K, Sakamaki K, Aoyama T, Suganuma N, Yamamoto N, Sato T, Cho H, Shiozawa M, Yoshikawa T, Rino Y, Kunisaki C, Imada T, Masuda M.
  • Journal Title: Oncology Letters Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Clinical significance of INHBA gene expression in patients with gastric cancer who underwent curative resection followed by adjuvant chemotherapy with S-1 (2017)
  • Author(s): Katayama Y, Oshima T, Sakamaki K, Aoyama T, Hayashi T, Yamada T, Sato T, Yamamoto Y, Kunisaki C, Shiozawa M, Yoshikawa T, Akaike M, Rino Y, Imada T, Masuda M
  • Journal Title: IN VIVO Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Clinical significance of IGF1R gene expression in patients with Stage II/III gastric cancer who receive curative surgery and adjuvant chemotherapy with S-1. (2016)
  • Author(s): Numata K, Oshima T, Sakamaki K, Yoshihara K, Aoyama T, Hayashi T, Yamada T, Sato T, Cho H, Shiozawa M, Yoshikawa T, Rino Y, Kunisaki C, Akaike M, Imada T, Masuda M.
  • Journal Title: Journal of Cancer Research and Clinical Oncology Volume: 142 Issue: 2 Pages: 415-422
  • DOI: 10.1007/s00432-015-2039-6
  • Peer Reviewed
• [Journal Article] Relation of INHBA Gene Expression to Outcomes in Gastric Cancer after Curative Surgery (2014)
  • Author(s): Oshima T, Yoshihara K, Aoyama T, Hasegawa S, Sato T, Yamamoto N, Akito N, Shiozawa M, Yoshikawa T, Numata K, Rino Y, Kunisaki C, Tanaka K, Akaike M, Imada T, Masuda M:
  • Journal Title: Anticancer Research Volume: 34 Pages: 2303-2309
  • Peer Reviewed
• [Journal Article] Impact of overexpression of Sushi repeat-containing protein X-linked 2 gene on outcomes of gastric cancer (2014)
  • Author(s): Yamada T, Oshima T, Yoshihara K, Sato T, Nozaki A, Shiozawa M, Ota M, Yoshikawa T, Akaike M, Numata K, Rino Y, Kunisaki C, Tanaka K, Imada T, Masuda M
  • Journal Title: Journal of Surgical Oncology Volume: 109 Issue: 8 Pages: 836-840
  • DOI: 10.1002/jso.23602
  • Peer Reviewed / Open Access
• [Presentation] Search for a combination of recurrence risk stratification markers after curative resection of gastric cancer (2017)
  • Author(s): Oshima T, Kimura Y, Sakamaki K, Miyagi Y, Yamanaka S, Sato T, Shiozawa M, Yoshikawa T, Rino Y, Oue N, Yasui W, Imada T, Masuda M
  • Organizer: American Association of cancer Research Annual Meeting 2017
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2017-03-31
  • Int'l Joint Research
• [Presentation] Search for biomarkers of Stage II/III gastric cancer using clinical specimens with the goal of personalized therapy (2017)
  • Author(s): 大島貴，木村弥生，坂巻顕太郎，宮城洋平，吉川貴己，森田智視，山中正二，長晴彦，塩澤学，利野靖，円谷彰，大上直秀，安井弥，今田敏夫，益田宗孝
  • Organizer: 第89回日本胃癌学会
  • Place of Presentation: 広島国際会議場，広島県広島市
  • Year and Date: 2017-03-08
  • Invited
• [Presentation] StageII/III胃癌根治切除後の再発リスク層別化マーカー検索 (2016)
  • Author(s): 大島貴，木村弥生，宮城洋平，坂巻顕太郎，山中正二，長晴彦，塩澤学，吉川貴己，利野靖，大上直秀，安井弥，今田敏夫，益田宗孝
  • Organizer: 第54回日本癌治療学会
  • Place of Presentation: パシフィコ横浜，神奈川県横浜市
  • Year and Date: 2016-10-20
• [Presentation] Statification Markers for the Risk of Recurrence after Curative Resection of Stage II or III Gastric Cancer (2016)
  • Author(s): 大島貴，木村弥生，坂巻顕太郎，宮城洋平，山中正二，吉川貴己，塩澤学，大上直秀，利野靖，安井弥，今田敏夫，益田宗孝
  • Organizer: 第75回日本癌学会
  • Place of Presentation: パシフィコ横浜，神奈川県横浜市
  • Year and Date: 2016-10-06
• [Presentation] Stratification Markers for the Risk of Recurrence after Curative Resection of Stage II/III Gastric Cancer. (2016)
  • Author(s): Oshima T, Sakamaki K, Miyagi Y, Cho H, Shiozawa M, Yoshikawa T, Rino Y, Kunisaki C, Tsuburaya A, Imada T, Masuda M
  • Organizer: American Association of cancer Research Annual Meeting 2016
  • Place of Presentation: New Orleans，USA
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] Stratification Markers for the Risk of Recurrence after Curative Resection of (2016)
  • Author(s): Oshima T, Sakamaki K, Miyagi Y, Cho H, Shiozawa M, Yoshikawa T, Rino Y, Kunisaki C, Tuburaya A, Imada T, Masuda M
  • Organizer: American Association of Cancer Research Annual Meeting
  • Place of Presentation: Ernest N. Morial Convention Center New Orleans, Louisiana, USA
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] StageII/III胃癌根治切除後のリスク層別化マーカー検索と臨床応用 (2015)
  • Author(s): 大島 貴，木村弥生，中居佑介，坂巻顕太郎，宮城洋平，山中正二，長晴彦，塩澤学，吉川貴己，利野靖，安井弥，今田敏夫，益田宗孝
  • Organizer: 第53回日本癌治療学会
  • Place of Presentation: 国立京都国際会館，京都
  • Year and Date: 2015-10-29 – 2015-10-31
  • Invited
• [Presentation] StageII/III胃癌根治切除後のリスク層別化マーカー検索と臨床応用 (2015)
  • Author(s): 大島貴，木村弥生，中居祐介，坂巻顕太郎，宮城洋平，山中正二，長晴彦，塩沢学，吉川貴己，利野靖，安井弥，今田敏夫，益田宗孝
  • Organizer: 日本癌治療学会
  • Place of Presentation: 国立京都国際会館（京都）
  • Year and Date: 2015-10-29
  • Invited
• [Presentation] Stratification Markers for the Risk of Recurrence after Curative Resection of Stage II or III Gastric Cancer and Potential Clinical Applications (2015)
  • Author(s): Oshima T, Sakamaki K, Miyagi Y, Cho H, Shiozawa M, Yoshikawa T, Rino Y, Kunisaki C, Tuburaya A Imada T, Masuda M
  • Organizer: American Society of Clinical Oncology Annual Meeting
  • Place of Presentation: McCormick Place, Chicago, Illinois, United States of America.
  • Year and Date: 2015-05-29 – 2015-06-29
• [Presentation] Stratification Markers for Risk of Recarrence after curative Resection of Stage II or III Gastric Cancer and Potential Clinical Applications. (2015)
  • Author(s): Oshima T, Sakamaki K, Miyagi Y, Cho H, Shiozawa M, Yoshikawa T, Rino Y, Kunisaki C, Tuburaya A, Imada T, Masuda M
  • Organizer: American Society of Clinical Oncology Annual Meeting
  • Place of Presentation: McCormick Place, Chicago, Illinois, USA
  • Year and Date: 2015-05-29
  • Int'l Joint Research
• [Presentation] Assessment of Recurrence Risk Stratification Markers after Resection of Stage II or III Gastric Cancer and Potential Clinical Applications. (2014)
  • Author(s): Oshima T, Sakamaki K, Miyagi Y, Shiozawa M, Kunisaki C, Yoshikawa T, Rino Y, Imada T, Masuda M
  • Organizer: American Association of Cancer Research Annual Meeting
  • Place of Presentation: Pennsylvania Convention Center, Philadelphia, Pennsylvania, United States of America.
  • Year and Date: 2014-04-18 – 2014-04-22
• [Book] 腫瘍内科 (2015)
  • Author(s): 大島 貴，吉川貴己
  • Total Pages: 144
  • Publisher: 科学評論社